褪黑素对大鼠应激性溃疡的保护及胃粘膜核因子-κB 活性的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (2): 211-214.

褪黑素对大鼠应激性溃疡的保护及胃粘膜核因子-κB 活性的影响

吴建胜, 吴金明, 王旦, 黄庆科, 陈向荣, 黄智铭, 林向飞, 陈民新, 韩清锡

温州医学院附属第一医院消化内科, 温州325000, 浙江

收稿日期:2004-11-23 修回日期:2005-01-06 出版日期:2005-02-06 发布日期:2020-11-18
通讯作者: 吴金明, 男, 博士, 副教授, 主要从事乙型肝炎的基础和临床研究。Tel:(0) 13587694167 E-mail:Phdwu0003@yahoo.com.cn
作者简介:吴建胜, 男, 主任医师, 主要从事激素与消化道疾病;内镜下小肠疾病诊治;胆胰疾病介入治疗。Tel:(0)13705880087
基金资助:
温州市科学技术局资助(NoY2003A023)

Effects of melatonin on gastric lesions and mucosal nuclear factor-κB (NF-κB) activation in rats with stress ulcer

WU Jian-sheng, WU Jin-ming, WANG Dan, HUANG Qing-ke, CHEG Xiang-rong, HUANG Zhi-min, LIN Xiang-fei, CHEN Min-xin, HAN Qing-xi

Department of Gastroenterology, the First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2004-11-23 Revised:2005-01-06 Online:2005-02-06 Published:2020-11-18

摘要/Abstract

摘要： 目的:探讨褪黑素(melatonin,MT) 对大鼠应激性溃疡的保护作用及对胃粘膜核因子-κB(NF-κB) 活化的影响, 阐明MT 的作用机制。方法:采用浸水-束缚(WIR) 应激实验复制大鼠应激性溃疡模型。应激前30 min, MT 5、20 mg°kg^-1组和应激组大鼠分别腹腔注射MT 5、20 mg°kg^-1 和等体积生理盐水。应激6 h 后, 观察各组大鼠胃粘膜病变清况, 对溃疡指数(UI) 进行评分, 同时检测各组大鼠胃粘膜内丙二醛(MDA) 含量和NF-κB 活化清况。结果:WIR 应激6 h 后, 与应激组比较, MT 5、20 mg°kg^-1 组大鼠胃粘膜病变明显减轻, UI 显著降低(P<0.01), 胃粘膜MDA 水平和NF-κB 活性也显著下降(P<0.01) 。MT 20 mg°kg^-1组大鼠UI(P<0.05) 和胃粘膜MDA水平(P<0.01) 均显著低于MT 5 mg°kg^-1 组, 但两组间胃粘膜NF-κB 活性无显著性差异(P>0.05) 。结论:MT 可有效预防应激性溃疡的发生, 其机制可能与MT 抑制氧化应激诱导的NF-κB 过度活化有关。

关键词: 褪黑素, 应激性溃疡, 核因子-κB,胃黏膜

Abstract: AIM: To investigate the effects of melatonin on gastric lesions and mucosal nuclear factor-κB (NF-κB) activation in rats with stress ulcer for elucidating the molecular mechanisms of melatonin intervention in vivo. METHODS: Stress ulcer was induced by water-immersion restraint (WIR) stress for 6 h.Melatonin (5 and 20 mg°kg^-1, ip) was administrated 30 min before WIR stress.After 6 h of WIR, rats were killed and the stomachs were removed.Ulcer index (UI) was used for evaluated macroscopic injury and gastric mucosal MDA contents and NF-κB activation were determined.RESULTS: Severe gastric lesions were induced after WIR stress for 6 h. There was a significantly increased in MDA contents (P<0.01) and NF-κB activation (P<0.01) in gastric mucosal in stress group.The gastric lesions in MT 5 mg°kg^-1 group and 20 mg°kg^-1 group were significantly relieved than that in the stress group after 30 min administration of melatonin (P<0.01).The UI (P<0.05) and mucosal MDA contents (P<0.01) in MT 20 mg°kg^-1 group were significantly lower than that in MT 5 mg°kg^-1 group.But there was no significantly difference in mucosal NF-κB activity between two groups.CONCLUSION: Melatonin can prevent the development of stress ulcer via a mechanism involving in reducing oxidative stress-induced NF-κB activation in gastric mucosa.

Key words: melatonin, stress ulcer, nuclear factor-κB, gastric mucosal

中图分类号:

R966

吴建胜, 吴金明, 王旦, 黄庆科, 陈向荣, 黄智铭, 林向飞, 陈民新, 韩清锡. 褪黑素对大鼠应激性溃疡的保护及胃粘膜核因子-κB 活性的影响[J]. 中国临床药理学与治疗学, 2005, 10(2): 211-214.

WU Jian-sheng, WU Jin-ming, WANG Dan, HUANG Qing-ke, CHEG Xiang-rong, HUANG Zhi-min, LIN Xiang-fei, CHEN Min-xin, HAN Qing-xi. Effects of melatonin on gastric lesions and mucosal nuclear factor-κB (NF-κB) activation in rats with stress ulcer[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(2): 211-214.

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