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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (2): 222-225.

• 研究原著 • 上一篇    下一篇

黄芪与绿豆对砒石染毒大鼠的影响

黎东明, 易震南, 梁标   

  1. 广东医学院呼吸疾病研究所, 湛江524023, 广东
  • 收稿日期:2004-12-01 修回日期:2005-01-30 出版日期:2005-02-06 发布日期:2020-11-18
  • 通讯作者: 黎东明, 女, 硕士, 副主任医师, 研究方向:慢性阻塞性肺疾病发病机制。Tel:0759-2387413 E-mail:golmclidongming@21cn.com

Influence of Astragalus membranceus and mung bean on arsenolite-induced rats

LI Dong-ming, YI Zhen-nan, LIANG Biao   

  1. Department of Respiratory Disease, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, Guangdong, China
  • Received:2004-12-01 Revised:2005-01-30 Online:2005-02-06 Published:2020-11-18

摘要: 目的:为探讨中药黄芪与绿豆对砒石染毒大鼠毒副作用的拮抗情况及其保护作用与金属硫蛋白(MT) 的关系。方法:用黄芪、绿豆及阳性对照药物氯化镉(CdCl2) 相比较, 运用镉饱和法及RT-PCR 测定肝细胞MT 的量及mRNA 的表达, 并通过检测丙氨酸氨基转移酶(ALT)、血尿素氮(BUN)、血清肌酐(SCR) 观察其对砒石引起的肾脏、肝脏的保护作用。结果:测的的MT 的量与其mRNA 表达的量具有一致性, 砒石、黄芪、绿豆均能诱导MT 的合成, 单纯砒石诱导MT 的量很少, 增加黄芪、绿豆后表达量明显增加, 黄芪与绿豆共同作用增加更明显(P<0.05) 。结论:黄芪与绿豆对砒石染毒大鼠金属硫蛋白均有影响, 对砒石毒性有一定的拮抗作用。

关键词: 黄芪, 绿豆, 砒石, 金属硫蛋白

Abstract: AIM: To investigate the effects of oral administration of Astragalus membranceus, mung bean and arsenolite on the toxic of the arsenolite-induced rats and the possible mechanisms with metallothionein (MT). METHODS: All the rats were oral administration with arsenolite.The Astragalus membranceus and mung bean were compared with the cadmium chloride which induced MT synthesis.The contents of MT were determined by cadmium saturation method, the liver mRNA levels for MT1, MT2 were detected by RT-PCR.The protective effects of renal and liver were observed by testing alanine aminotransferase (ALT), blood urea nitrogen (BUN) and serum creatinine (SCR).RESULTS: The contents of MT were accorded with the mRNA expression of MT1, MT2.Arsenolite, Astragalus membranceus and mung bean could induce the synthesis of MT, but the contents of MT which arsenolite induced were trace.The contents of MT significantly increased after oral administration of Astragalus membranceus and mung bean, especially in the Astragalus membranceus group (P<0.05).CONCLUSIONS: Astragalus membranccus and mung bean has a significantly inference in the MT and an antagonistic action on arsenolite-induced rats.

Key words: Astragalus membranceus, mung bean, arsenolite, metallothionein

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