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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (2): 226-229.

• 研究原著 • 上一篇    下一篇

乙醇对体外培养的2.2.15 细胞中HBV 复制和表达的影响

吴金明, 吴建胜, 陈民新, 韩清锡   

  1. 温州医学院第一附属医院消化内科, 温州325000, 浙江
  • 收稿日期:2004-11-21 修回日期:2004-12-30 出版日期:2005-02-06 发布日期:2020-11-18
  • 通讯作者: 吴金明, 男, 博士, 副教授, 副主任医师, 主要从事乙型肝炎的基础和临床研究。Tel:0577-88134169 E-mail:phdwu0003@yahoo.com.cn

Effects of ethanol on replication and expression of HBV in vitro in 2.2.15 cells

WU Jin-ming, WU Jian-sheng, CHEN Min-xin   

  1. Department of Gastroenterology, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, Zhejiang, China
  • Received:2004-11-21 Revised:2004-12-30 Online:2005-02-06 Published:2020-11-18

摘要: 目的:探讨乙醇对乙型肝炎病毒复制和表达的影响。方法:采用人HBV DNA 转染的细胞株2.2.15 细胞作为研究对象, 以不同浓度的乙醇进行干预, 同时设立阴性对照、3TC 阳性对照, 观察乙醇对HBV 表达和复制影响, 并检测细胞内抗病毒基因MxA 的表达情况。结果:乙醇能够加强培养上清液中乙肝表面抗原、e 抗原以及细胞内核心抗原的表达, 而3TC 组三种抗原均无明显影响;Southern 印迹显示, 乙醇于200 mmol°L-1时明显加强HBV DNA 的复制, 3TC 于2 μmol°L-1 浓度时则明显抑制HBVDNA 的复制;乙醇于40 mmol°L-1 时, MxAmRNA 表达显著加强, 3TC 于2 μmol°L-1 时MxAmRNA 亦明显表达增加。结论:乙醇能刺激乙肝表面抗原、e 抗原以及核心抗原的表达, 同时细胞内抗病毒基因MxA表达亦增加, 可能与乙醇非特异性增强细胞内酶的活性, 加强细胞内合成代谢有关;乙醇可以促进HBV 复制, 但机制有待研究。

关键词: 乙型肝炎病毒, 乙醇, 复制, 表达

Abstract: AIM: To explore the effects of ethanol on replication and expression of HBV, so as to effectively guide the anti-HBV treatment in clinic.METHODS: 2. 2.15 cells were cultured in vitro.Ethanol of different concentrations was added into the supernatant and 3TC was used as the positive control.And there was no drug added in the negative control group.Effect of ethanol on HBV expression and replication was examined and the expression of antiviral gene MxA was also tested.RESULTS: Ethanol promoted the expression of intracellular HBsAg, HBeAg and intracellular HBcAg.But 3TC revealed no significant effect on all of them.Southern blotting showed that ethanol at 200 mmol°L-1 enhance the replication of HBV DNA, but 3TC inhibited the replication of HBV DNA at 2 μmol°L-1.Ethanol at 40 mmol°L-1 promoted the expression of MxA, while 3TC at 2 μmol°L-1 also stimulated the expression of MxA. CONCLUSION: Ethanol can induce the overexpression of HBsAg, HBeAg and HBcAg, and enhance the expression of antiviral gene MxA which may increase the activity of enzymes of synthetic metabolism.Ethanol can promote replication of HBV DNA, but the mechanism is unclear and need to be studied.

Key words: Hepatitis B virus, ethanol, replication, expression

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