参附注射液对大鼠急性缺血/再灌注损伤心肌核转录因子-κB 的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (3): 306-309.

参附注射液对大鼠急性缺血/再灌注损伤心肌核转录因子-κB 的影响

张本静, 王焱林, 王成夭

武汉大学中南医院麻醉科, 武汉430071, 湖北

收稿日期:2004-11-17 修回日期:2005-02-03 出版日期:2005-03-26 发布日期:2020-11-18
通讯作者: 王焱林, 男, 教授, 博士生导师, 主要从事麻醉与循环基础和临床研究。Tel:027-62402493 E-mail:WYL0342@sina.com
作者简介:张本静, 女, 博士研究生, 主要从事麻醉与循环基础和临床研究。Tel:027-62974512 E-mail:zhangbenjing@tom.com
基金资助:
湖北省卫生厅资助项目(NoJXIB090)

Effects of shenfu injection on nuclear factor-κB during myocardial ischemia/reperfusion injury in rats

ZHANG Ben-jing, WANG Yan-lin, WANG Cheng-yao

Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China

Received:2004-11-17 Revised:2005-02-03 Online:2005-03-26 Published:2020-11-18

摘要/Abstract

摘要： 目的:探讨参附注射液保护大鼠心肌缺血/再灌注损伤作用与机理。方法:采用大鼠心脏左冠状动脉前降支缺血/再灌注模型, 缺血30 min, 再灌注60 min。24 只SD 大鼠随机分为3 组:假手术组;缺血/再灌注组;参附注射液组。酶联免疫吸附实验法检测血浆中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6) 浓度, 免疫印记法测心肌核转录因子-κB(NF-κB) 活性水平, 电镜观察心肌超微结构。结果:缺血再灌注组心肌NF-κB 的活性, 血浆TNF-a、IL-6 浓度较假手术组明显上升(P<0.01)。参附注射液组中上述指标与缺血/再灌注组比较均显著下降(P<0.01), 电镜下参附注射液组中心肌超微结构较缺血再灌注组明显减轻并接近正常。结论:参附注射液通过抑制缺血心肌中NF-κB 的活性, 降低促炎因子水平, 对心肌起保护作用。

关键词: 参附注射液, 缺血再灌注损伤, 核转录因子-κB, 肿瘤坏死因子-α, 白细胞介素-6

Abstract: AIM: To study the protective effects of shenfu injection on myocardial ischemia/reperfusion (I/R) injury in rats and its potential mechanisms.METHODS: Myocardial ischemia/reperfusion was produced by tying and untying of left anterior descending coronary artery. Ischemia lasted for 30 min and reperfusion for 60 min. Twenty-four healthy male SD rats weighing 230-280 g were randomly divided into three groups (n=8 in each): sham-operation group, I/R group, and shenfu group which the shenfu injection (10 ml°kg^-1) were injected intraperitoneally 30 min before ischemia.The plasma concentration of tumor necrosis factor-α(TNF-α), interleukin- 6 (IL-6) were measured by ELISA.The heart was harvested and levels of the nuclear factor kappaB (NF- κB) activity were determined by Ecl-western blot analysis and ultrastructures were observed by electron microscopy. RESULTS: NF-κB binding activity in myocardial nuclear and the plasma concentration of IL-6, TNF-αwere significantly increased in I/R group than that in the sham-operation group (P<0.01), and they were markedly reduced in shenfu group compared with I/R group (P<0.01).In addition, electron microscopic examination showed more serious injury of the myocardium ultrastructure in I/R group, while in shenfu group the myocardial ultrastructure could improve close to normal.CONCLUSION: Shenfu injection can inhibit NF-κB activity in postischemic myocardium and lead to down-regulation of proinflammtory cytokine expression, which may be one of molecular mechanisms of shenfu injection in cardioprotection.

Key words: shenfu injection, ischemia, reperfusion, nuclear factor-kappa B, tumor necrosis factor-alpha, interleukin-6

中图分类号:

R966

张本静, 王焱林, 王成夭. 参附注射液对大鼠急性缺血/再灌注损伤心肌核转录因子-κB 的影响[J]. 中国临床药理学与治疗学, 2005, 10(3): 306-309.

ZHANG Ben-jing, WANG Yan-lin, WANG Cheng-yao. Effects of shenfu injection on nuclear factor-κB during myocardial ischemia/reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(3): 306-309.

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