罗红霉素对小鼠接触性皮炎及 T 细胞功能的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (4): 382-386.

罗红霉素对小鼠接触性皮炎及 T 细胞功能的影响

刘康, 徐强¹

中国药科大学中药药理教研室, 南京 210038, 江苏;
¹南京大学生命科学学院医药生物技术国家重点实验室, 南京 210093, 江苏

收稿日期:2005-02-23 修回日期:2005-03-19 出版日期:2005-04-26 发布日期:2020-11-19
通讯作者: 刘康,女, 讲师, 博士生, 研究方向:免疫药理学。Tel:025-85322594 E-mail:liukangcpu@yahoo.com.cn
作者简介:徐强, 教授, 博士生导师, 研究方向:免疫病理学及免疫药理学。Tel:025-83597620

Effects of roxithromycin on contact sensitivity and functions of T Lympho-cytes

LIU Kang, XU Qiang¹

Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, Jiangsu,China;
¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093, Jiangsu, China

Received:2005-02-23 Revised:2005-03-19 Online:2005-04-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 研究罗红霉素对接触性皮炎及 T 细胞功能的影响。方法: 使用 2, 4, 6-三硝基氯苯诱导小鼠接触性皮炎, 以双向混合淋巴细胞反应及刀豆蛋白诱导脾 T 细胞增殖, MTT 法检测活细胞数量。采用 RT-PCR 方法检测细胞因子 mRNA 表达。结果: 20mg°kg^-1 罗红霉素效应相灌胃给药明显抑制 2, 4,6-三硝基氯苯诱导的小鼠接触性皮炎(P<0.01)。25μg°ml^-1 罗红霉素显著降低双向混合淋巴细胞反应(P<0.05), 5、25μg°ml^-1 罗红霉素明显抑制刀豆蛋白诱导的脾细胞存活、增殖(P<0.05, P <0.01)及T-bet 、IL-2 及 IFN-γ的mRNA 表达。结论: 罗红霉素能抑制 2, 4, 6-三硝基氯苯诱导的接触性皮炎, 可能与其抑制T 淋巴细胞存活、活化及增殖有关。

关键词: 罗红霉素, 接触性皮炎, 混合淋巴细胞反应, T 淋巴细胞增殖, mRNA 表达

Abstract: AIM: To observe effects of roxithromycin on contact sensitivity induced by picryl chloride (PCl) and on the functions of T cells.METHODS: Contact sensitivity was induced with picryl chloride, two-way mixed lymphacyte reaction and Concanavalin A were used to induce T cell proliferation. The viability and cell pro-liferation were examined by MTT method, mRNA expres-sion was analyzed by RT-PCR and densitometry. RESULTS: Roxithromycin 20 mg°kg^-1 significantly sup-pressed contact sensitivity induced by picryl chloride in mice. In vitro assay, roxithromycin dose-dependently in-hibited splenic T lymphocyte proliferation induced by con-canavalin A, 5 and 25μg°ml^-1 of roxithromycin sup-pressed mRNA expressions of T-bet, IL-2 and IFN-γ.25 μg°ml^-1 of roxithromycin also decreased two-way mixed lymphocyte reaction.CONCLUSION: Roxithromycin suppresses picryl chloride-induced contact sensitivity. The inhibitory effect may be related to the suppression on the viability, activation and proliferation of T lympho-cytes.

Key words: roxithromycin, contact sensitivity, two-way mixed lymphacyte reaction, T lymphocyte prolifera-tion, mRNA expression

中图分类号:

R978.1

刘康, 徐强. 罗红霉素对小鼠接触性皮炎及 T 细胞功能的影响[J]. 中国临床药理学与治疗学, 2005, 10(4): 382-386.

LIU Kang, XU Qiang. Effects of roxithromycin on contact sensitivity and functions of T Lympho-cytes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(4): 382-386.

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