欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (6): 617-621.

• 研究原著 • 上一篇    下一篇

土贝母皂苷对人低分化上皮样鼻咽癌细胞(CNE-2Z) 微管的作用

宋刚1,2, 马润娣1, 于立坚1   

  1. 1湛江海洋大学广东省海洋药物研究与开发重点实验室, 湛江524025, 广东;
    2华南理工大学食品与生物工程学院, 广州510640, 广东
  • 收稿日期:2005-04-04 修回日期:2005-05-20 出版日期:2005-06-26 发布日期:2020-11-12
  • 通讯作者: 马润娣, 女, 研究员, 博士生导师, 研究方向:肿瘤药物作用的靶点。Tel:0759-2382424
  • 作者简介:宋刚, 男, 博士研究生。

Effects of tubeimoside isolated from Bolbostemma paniculatum on microtubule in CNE-2Z cells

SONG Gang1,2, MA Run-di1, YU Li-jian1   

  1. 1Guangdong Provincial Key Laboratory of Marine Materia Medica, Zhanjiang Ocean University, Zhanjiang 524025, Guangdong, China;
    2College of Food and Biological Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China
  • Received:2005-04-04 Revised:2005-05-20 Online:2005-06-26 Published:2020-11-12

摘要: 目的: 探讨土贝母皂苷(皂苷) 对人低分化上皮样鼻咽癌细胞株(CNE-2Z) 微管的影响。方法: 采用细胞免疫荧光法观察皂苷处理的CNE-2Z 细胞微管的变化, 蛋白免疫印迹法分析皂苷处理的CNE-2Z细胞中聚合和未聚合微管蛋白含量的变化。结果: 免疫荧光图像结果表明, 皂苷作用的CNE-2Z 细胞丧失正常的微管网络, 蛋白免疫印迹结果证实皂苷使CNE-2Z 细胞未聚合的微管蛋白比例增加, 而且这一作用与剂量和时间呈正相关。结论: 皂苷能抑制CNE-2Z 细胞内微管的聚合, 提示皂苷是一种有效的抗微管剂。

关键词: 土贝母, 土贝母皂苷, 微管, 微管蛋白, 抗微管剂, 鼻咽癌细胞株

Abstract: AIM: To examine the effects of tubeimoside isolated from Bolbostemma paniculatum on microtubule in low differentiated nasopharyngeal carcinoma CNE-2Z cells. METHODS: The interactions of tubeimoside with microtubule in CNE-2Z cells were investigated by immunofluorescence microscopy, and the interactions of tubeimoside with tubulin in CNE-2Z cells were detected by Western blotting. RESULTS: Tubeimoside (25 μmol·L-1, 3 h) was sufficient to cause the microtubular network disruption. Western blot analysis revealed that the proportion of cytosolic tubulin of cells treated with tubeimoside increased in a time-dependent and concentration-dependent manner. CONCLUSION: Tubeimoside is an effective antimicrotubule agent.

Key words: Bolbostemma paniculatum, tubeimoside, microtubule, tubulin, antimicrotubule agent, CNE-2Z cells

中图分类号: