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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (6): 651-654.

• 研究原著 • 上一篇    下一篇

心房颤动患者氯电流相关通道CIC-3 基因表达的研究

吴家斌, 许春萱1, 张建成, 陈林, 林立芳, 胡锡衷   

  1. 福建医科大学省立临床学院, 福建省立医院内科, 福州350001, 福建; 1福建省心血管病研究所内科, 福州350001, 福建
  • 收稿日期:2005-03-14 修回日期:2005-04-07 出版日期:2005-06-26 发布日期:2020-11-12
  • 通讯作者: 许春萱, 男, 主任医师, 博士研究生导师, 研究方向:心电生理与起搏。Tel:0591-87557768 E-mail: xuchunxuan@163.com
  • 作者简介:吴家斌, 男, 硕士研究生, 主治医师, 研究方向:心电生理与起博。Tel:(0) 13805008498 E-mail: wujiabin@mail.china.com

Study of chloride channel ClC-3 gene expression in patient with atrial fibrillation

WU Jia-bin, XU Chun-xuan, ZHANG Jian-cheng, CHEN Lin, LIN Li-fang, HU Xi-zhong   

  1. Department of Internal Medicine, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, Fujian, China; 1Fujian Provincial Research Institute of Cardiovascular Diseases, Fuzhou 350001, Fujian, China
  • Received:2005-03-14 Revised:2005-04-07 Online:2005-06-26 Published:2020-11-12

摘要: 目的: 研究心房颤动(AF) 患者心房组织氯电流相关通道ClC-3 的基因表达, 探讨AF 时心房组织ClC-3 的mRNA 表达改变及其意义。方法: 将71 例风湿性心瓣膜病接受换瓣手术患者分为3 组:窦性心律组(SR 组) 31 例, 阵发性房颤组(PAF 组) 7 例,持续性房颤组(CAF 组) 33 例, 术中获取右心耳组织, 应用半定量逆转录-聚合酶链反应(RT-PCR) 技术检测心房组织ClC-3 的mRNA 表达的相对含量。结果: 与SR 组相比, PAF 组心房组织中ClC-3 的mRNA表达增加, 但无显著的统计学意义(P >0.05), CAF组的表达明显增加(P <0.01), 并与AF 时程呈明显正相关(r =0.376, P =0.001) 。结论: 房颤患者ClC-3 的mRNA 表达水平的增加可能是细胞容量调节的氯电流(ICl. VOL) 上调的分子基础。

关键词: 心房颤动, 氯通道, 半定量逆转录-聚合酶链反应, 基因表达

Abstract: AIM: To investigate alterations in gene expression of chloride channel ClC-3 in patients with atrial fibrillation (AF). METHODS: Right atrial appendages specimen were obtained from 33 patients with chronic AF(CAF group), 7 with paroxysmal AF(PAF group) and 31 matched controls in sinus rhythm (SR group) who underwent open-heart surgery. In this study, the semi-quantitative polymerase chain reaction (RT-PCR) was used to examine the mRNA expression of ClC-3 channel in atrial tissue. RESULTS: The content of ClC-3 mRNA in CAF group was higher than that in SR group(P <0.01), and it was correlated with AF duration (r =0.376, P = 0.001). There was no significant difference in the gene expression of ClC-3 between PAF group and SR group, although the content of ClC-3 mRNA was slightly higher than that in SR group (P >0.05). CONCLUSION: The increased gene expression of chloride channel ClC-3 may be served as the molecular basis of ICl.VOL remodeling in patients with atrial fibrillation (AF).

Key words: atrial fibrillation, chloride channel, RT-PCR, gene expression

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