D-半乳糖对雄性大鼠腰椎骨密度和血清睾酮含量的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (6): 670-673.

D-半乳糖对雄性大鼠腰椎骨密度和血清睾酮含量的影响

罗红梅, 崔燎¹, 吴铁¹

广东医学院生理科学实验室,; ¹药理教研室, 湛江524023, 广东

收稿日期:2005-05-31 修回日期:2005-06-13 出版日期:2005-06-26 发布日期:2020-11-12
作者简介:罗红梅, 硕士, 助教, 主要从事骨药理学研究。Tel:0759-2388424 E-mail: lhmjyb@yahoo.com.cn

Effects of D-galactose on lumber vertebra density and serum testosterone concentration in male rats

LUO Hong-mei, CUI Liao¹, WU Tie¹

Laboratory of Physiological Science, ¹Department of Pharmacology, Guangdong Medical College, Zhangjiang 524023, Guangdong, China

Received:2005-05-31 Revised:2005-06-13 Online:2005-06-26 Published:2020-11-12

摘要/Abstract

摘要： 目的: 观察D-半乳糖对雄性大鼠腰椎骨密度的影响及其产生的原因。方法: 12 只6 月龄雄性大鼠随机分成2 组, 对照组和D-半乳糖组, 每组6只。对照组每天皮下注射生理盐水, D-半乳糖组皮下注射5 %的D-半乳糖, 剂量为100 mg·kg^-1, 连续给药3 个月后, 心脏取血处死大鼠, 取出第4 腰椎, 放入福尔马林中固定;同时取出睾丸, 放入固定液中固定。取出的血液静置一段时间后, 离心获取血清, 放入-70 ℃ 冰箱中冷冻保存备用。第4 腰椎经梯度脱水后, 塑料包埋、切片, 用图象分析仪上进行骨量分析。睾丸制作普通石蜡切片, 光镜下观察。冷冻血清, 解冻后用放射免疫法测定血清睾酮促、黄体生成素(LH) 含量。结果: D-半乳糖组大鼠腰椎骨出现骨质疏松。与对照组相比, D-半乳糖组血清睾酮含量明显降低, 而黄体生成素无明显变化, 睾丸切片呈现老化表现, 但黄体生成素无明显变化。结论: D-半乳糖会导致雄性大鼠骨质疏松, 其原因可能是影响了下丘脑-垂体-性腺轴的功能, 导致血清睾酮含量降低, 最终引起骨质疏松。

关键词: D-半乳糖, 骨质疏松, 睾酮, 黄体生成素

Abstract: AIM: To observe the effects of D-galactose on the lumber vertebra body and investigate the reasons. METHODS: 12 rats at age of 6 months were divided into two groups, control group and D-galactose group (n =6 in each). The control group were administered saline solution sc, and the D-galactose group were administered 5 %D-galactose solution sc at dose of 100 mg·kg^-1. After 3 month, the rats were killed by exsanguination from heart. The fourth lumber vertebra was taken and immerged in formalin. The testicle were taken and immerged in formalin at the same time. The blood serum was collected by centrifugating the collected blood after resting for a while, and it was preserved in refrigeratory at the degree of-70 ℃.The vertebra body were embedded in plastic and sliced up after being dehydrated step by step with different concentration ethanol. The slices were analyzed under the image analysis apparatus. The testicle were made into paraffin slices and observed under the common microscope. The concentration of serum testosterone and luteinizing hormone were measured by radio-immunity assay. RESULTS: The lumber vertebra body in D-galactose group appeared osteoporosis. The serum testosterone hormone concentrations of D-galactose rats were significantly decreased. And the microstructure of testicle present aging change, but no change of serum LH concentration was observed. CONCLUTION: D-galactose can cause the osteoporosis in male rats, which may be related to affect the function of thalamus-pituitary-testicle axis, decrease the content of testosterone of D-galactose.

Key words: D-galactose, osteoporosis testosterone, luteinizing hormone (LH)

中图分类号:

R965

罗红梅, 崔燎, 吴铁. D-半乳糖对雄性大鼠腰椎骨密度和血清睾酮含量的影响[J]. 中国临床药理学与治疗学, 2005, 10(6): 670-673.

LUO Hong-mei, CUI Liao, WU Tie. Effects of D-galactose on lumber vertebra density and serum testosterone concentration in male rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(6): 670-673.

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