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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (7): 750-754.

• 研究原著 • 上一篇    下一篇

计算机辅助设计抗肿瘤凋亡基因livin的反义核酸

张华东, 袁守军, 陈惠鹏   

  1. 军事医学科学院放射医学研究所, 北京100850
  • 收稿日期:2005-04-26 修回日期:2005-06-23 出版日期:2005-07-26 发布日期:2020-11-10
  • 通讯作者: 袁守军,男, 博士后, 副研究员, 研究方向:肿瘤药理和药物安全评价。Tel:010-66930271 E-mail: yuansj@nic.bmi.ac.cn
  • 作者简介:张华东, 男, 博士生。Tel:010-66931215 E-mail: xulp@nic.bmi.ac.cn

Design of livin antisense oligonucleotides with computer

ZHANG Hua-dong, YUAN Shou-jun, CHEN Hui-peng   

  1. Department of Pharmacology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
  • Received:2005-04-26 Revised:2005-06-23 Online:2005-07-26 Published:2020-11-10

摘要: 目的: 研究利用计算机辅助设计抗肿瘤细胞凋亡基因livin mRNA 的反义核酸。方法: 利用RNAdraw 和Sfold 两种软件进行livin mRNA 反义核酸的二级结构预测, 并转染肿瘤细胞HeLa, 诱导细胞凋亡。结果: 用这种方法设计出5 个反义核酸, 它们能够有效地抑制肿瘤细胞HeLa 的生长, 促进其凋亡。结论: 利用这两种计算机软件设计livin mRNA反义核酸是可行的, 能够有效的促进细胞凋亡。

关键词: mRNA 二级结构, RNAdraw, Sfold, livin, 反义核酸

Abstract: AIM: Antisense oligonucleotides against livin were designed with computer software. METHODS: Antisense oligonucleotides were designed according to the secondary structure of livin mRNA which was simulated with RNAdraw and Sfold. And then these oligonucleotides were transfected into Hela cells for inducing apoptosis. RESULTS: Five antisense oligonucleotides were designed using RNAdraw and Sfold, and effectively induced Hela cell apoptosis. CONCLUSION: The approach is effective and feasible to design antisense oligonucleotides by means of calculation with two kinds of software.

Key words: the secondary structure of mRNA, RNAdraw, Sfold, livin antisense ODNs

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