BXSB 狼疮小鼠肾组织TGF-β1异常表达及甲泼尼龙干预作用

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (7): 799-803.

BXSB 狼疮小鼠肾组织TGF-β₁异常表达及甲泼尼龙干预作用

王美美, 朱小霞, 孙茹蓉, 徐雷艇

东南大学附属中大医院风湿免疫科, 南京210009, 江苏

收稿日期:2005-05-12 修回日期:2005-06-29 出版日期:2005-07-26 发布日期:2020-11-10
通讯作者: 王美美, 女, 教授, 硕士生导师, 研究方向为系统性红斑狼疮的发病机制。Tel:025-83272142 E-mail: Wmm-3272142@sohu.com

Inhibition effects of methylprednisolone on over-expression of transforming growth factor-β₁ (TGF-β₁) in kidneys of BXSB mice

WANG Mei-mei, ZHU Xiao-xia, SUN Ru-rong, XU Lei-ting

Department of Reumatology, Zhongda Hospital, Affiliated to Southeast University, Nanjing 210009, Jiangsu, China

Received:2005-05-12 Revised:2005-06-29 Online:2005-07-26 Published:2020-11-10

摘要/Abstract

摘要： 目的: 研究转化生长因子-β₁ (TGF-β₁) 在BXSB小鼠狼疮肾炎(LN) 中的作用及甲泼尼龙(MPS) 是否可通过影响TGF-β₁ 表达而改善LN 。方法: 用逆转录-聚合酶链反应技术(RT-PCR) 和免疫组织化学方法检测BXSB 狼疮小鼠及BALB /c 小鼠肾组织TGF-β1 mRNA 及蛋白的表达情况, 并用甲泼尼龙对BXSB小鼠进行体内干预, 观察甲泼尼龙对BXSB 小鼠肾脏TGF-β₁ 表达的影响及各组小鼠24 h 尿蛋白量的变化。结果: RT-PCR 检测到BXSB 小鼠肾组织TGF-β₁mRNA 表达明显高于BALB /c 小鼠(P <0.01); 其中, BXSB 小鼠治疗组肾组织TGF-β₁mRNA 表达量显著低于对照组(P <0.01) 。BXSB 小鼠治疗组24 h尿蛋白量明显低于对照组(P <0.01) 。免疫组织化学检测示TGF-β₁ 表达于BXSB 小鼠肾小球系膜细胞、肾小管上皮细胞、血管内皮细胞、肾间质及肾小球浸润的炎症细胞胞质内, 其中BXSB 小鼠治疗组较对照组表达明显减弱, 而BALB /c 小鼠仅在少数肾小管上皮细胞有微弱表达, 且图像半定量分析显示各组有显著性差异(P <0.01) 。结论: TGF-β₁ 可能参与LN 发病;甲泼尼龙可能通过抑制肾组织TGF-β₁ 的表达来改善LN。

关键词: 甲泼尼龙, 狼疮肾炎, 肾组织, 转化生长因子-β₁, 小鼠

Abstract: AIM: To investigate the relationship between TGF-β₁ over-expression and lupus nephritis(LN) in BXSB mice and whetherMPS ameliorates LN by inhibiting the over-expression of TGF-β₁. METHODS: BXSB mice (experiment group, n = 6) were treated (i.p.) with MPS (25 mg·kg^-1·d^-1) dissolved in N. S for 3 weeks. The other age and sex-matched BXSB mice (n =6) and BALB /c mice (n =6) received N. S. alone. Proteinuria production was evaluated once a week. The expression of TGF-β₁ in the kidney was investigated by means of immunohistochemistry and RT-PCR. RESULTS: MPS significantly reduced proteinuria of BXSB mice (P<0.01). TGF-β₁ was strongly expressed in kidneys of all BXSB mice, while weak expressions were found in kidneys of BALB /c mice. A significant lower expression of TGF-β₁ was found in the BXSB experiment group compared with BXSB control group (P <0.01). CONCLUSION: The over-expression of TGF-β₁ may aggravate LN, while MPS partially ameliorates LN by inhibiting the expression of TGF-β₁.

Key words: methylprednisolone, lupus nephritis, kidney, transforming growth factor-β₁, mouse

中图分类号:

R977.1

王美美, 朱小霞, 孙茹蓉, 徐雷艇. BXSB 狼疮小鼠肾组织TGF-β₁异常表达及甲泼尼龙干预作用[J]. 中国临床药理学与治疗学, 2005, 10(7): 799-803.

WANG Mei-mei, ZHU Xiao-xia, SUN Ru-rong, XU Lei-ting. Inhibition effects of methylprednisolone on over-expression of transforming growth factor-β₁ (TGF-β₁) in kidneys of BXSB mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(7): 799-803.

参考文献

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BXSB 狼疮小鼠肾组织TGF-β₁异常表达及甲泼尼龙干预作用

Inhibition effects of methylprednisolone on over-expression of transforming growth factor-β₁ (TGF-β₁) in kidneys of BXSB mice

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