5-氟脲嘧啶对口腔癌Tb细胞增殖活性及钾通道功能的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (8): 890-893.

5-氟脲嘧啶对口腔癌Tb细胞增殖活性及钾通道功能的影响

马宇光, 武力, 王育成, 马大昌, 王丽京¹

兰州大学第一医院乳腺病科, 兰州 730000, 甘肃;
¹广东药学院基础医学院组胚教研室, 广州 510224, 广东

收稿日期:2005-05-31 修回日期:2005-06-05 发布日期:2020-11-22
通讯作者: 王丽京,女,教授,硕士生导师,研究方向:肿瘤病理。Tel:020-39340880 E-mail:wanglijing62@yahoo.com.cn
作者简介:马宇光,女,硕士,研究方向:肿瘤病理。Tel:029-83765732 E-mail:xiemanjiang@163.com

Effects of 5-Fluorouracil on cell proliferation and potassium channel function in oral cancer cell line Tb

MA Yu-guang, WU Li, WANG Yu-cheng, MA Da-chang, WU Li, WANG Li-jing¹

Department of Breast Diseases, the First Affiliated Hospital of LanzhouUniversity, Lanzhou 730000, Gansu, China;
¹Department of Histology and Embryology, Guang Dong College of Pharmacy, Guangzhou 510224, Guangdong, China

Received:2005-05-31 Revised:2005-06-05 Published:2020-11-22

摘要/Abstract

摘要： 目的: 观察5-氟脲嘧啶(5-FU)对口腔癌Tb细胞增殖活性以及钾离子通道功能的影响,进一步揭示5-FU的抗肿瘤机理,并初步探讨钾离子通道与肿瘤细胞增殖的关系。方法: 以5-FU作用Tb细胞24h后,应用MTT比色法与平板克隆形成实验观察Tb细胞的增殖活性,同时以全细胞膜片钳技术检测Tb细胞膜电位与跨膜钾电流的改变。结果: 与空白对照组相比,5-FU能明显抑制Tb细胞的增殖活性,并且可使细胞膜电位发生去极化改变,跨膜钾电流显著降低。结论: 5-FU在抑制Tb细胞增殖的同时钾通道功能也显著降低,提示钾离子通道可能参与调控5-FU抑制Tb细胞增殖的过程。

关键词: 5-氟脲嘧啶, Tb 细胞, 增殖, 钾通道, 膜片钳技术

Abstract: AIM: To evaluate the effects of 5-Fluorouracil (5-FU) on cell proliferation and potassium channel function in oral cancer cell line Tb.METHODS: After treatment with 5-FU for 24 h, the proliferation of Tb cells was determined by MTT and clone formation assay, the membrane potential and potassium channel current of Tb cells were recorded by whole-cell patch clamp technique.RESULTS: Compared with the control group (CON), 5-FU inhibited proliferation of the Tb cells.At the same time the membrane was depolarized and the potassium channel current significantly decreased compared with the CON group.CONCLUSION: Potassium channel may play an important role in 5-FU-inhibited the proliferation of Tb cells.

Key words: 5-Fluorouracil (5-FU), Tb cells, proliferation, potassium channel, patch clamp technique

中图分类号:

R979.14

马宇光, 武力, 王育成, 马大昌, 王丽京. 5-氟脲嘧啶对口腔癌Tb细胞增殖活性及钾通道功能的影响[J]. 中国临床药理学与治疗学, 2005, 10(8): 890-893.

MA Yu-guang, WU Li, WANG Yu-cheng, MA Da-chang, WU Li, WANG Li-jing. Effects of 5-Fluorouracil on cell proliferation and potassium channel function in oral cancer cell line Tb[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(8): 890-893.

参考文献

1 Wonderlin WF, Strobl JS.Potassium channels, proliferation and G1 progression[J].J Membr Biol, 1996;154:91-107
2 Bordey A, Sontheimer H.Electrophysiological properties of human astrocytic tumor cells in situ:enigma of spiking glial cells [J].J Neurophysiol, 1998;79:2782-93
3 Yao X, Kwan HY.Activity of voltage-gated K+channels is associated with cell proliferation and Ca²⁺influx in carcinoma cells of colon cancer[J].Life Sci, 1999;65:55-62
4 Klimatcheva E, Wonderlin WF.An ATP-sensitive K+current that regulates progression through early G1 phase of the cell cycle in MCF-7 human breast cancer cells[J].J Membr Biol, 1999;171:35-46
5 吴军正, 陈建元, 李峰, 李焰, 张洪.舌癌脑转移细胞系Tb的建立及形态和生长特性[J].实用口腔医学杂志, 1999; 15:452-3
6 Fu ZJ, Xie MJ, Zhang LF, Cheng HW,Ma J.Differential activation of potassium channels in cerebral and hindquarter arteries of rats during simulated microgravity[J].Am J Physiol Heart Circ Physiol, 2004;287:H1505-15
7 Pancrazio JJ, Viglione MP, Tabbara IA, Kim YI.Voltage-dependent ion channels in small-cell lung cancer cells[J].Cancer Res, 1989;49:5901-6
8 Xu R, Roh SG, Loneragan K, Pullar M, Chen C.Human GHRH reduces voltage-gated K+currents via a non-cAMP-dependent but PKC-mediated pathway in human GH adenoma cells [J].J Physiol, 1999;520:697-707
9 Meyer R, Heinemann SH.Characterization of an eag-like potassium channel in human neuroblastoma cells [J].J Physiol, 1998;508:49-56
10 Kohler R, Degenhardt C, Kuhn M, Runkel N, PaulM, Hoyer J.Expression and function of endothelial Ca²⁺-activated K+channels in human mesenteric artery:a single-cell reverse transcriptase-polymerase chain reaction and electrophysiological study in situ[J].Circ Res, 2000;87:496-503
11 Skryma R, Van Coppenolle F, Dufy-Barbe L, Dufy B, Prevarskaya N.Characterization of Ca²⁺-inhibited potassium channels in the LNCaP human prostate cancer cell line[J].Receptors Channels, 1999;6:241-53
12 韩英, 郎兵, 吴汉平, 周炜, 高瞻, 周士胜, 等.人胃癌SGC7901 细胞膜钾离子通道的特性[J].第四军医大学学报, 2002;23:608-10
13 Jensen BS, Odum N, Jorgensen NK, Christophersen P, Olesen SP.Inhibition of T cell proliferation by selective block of Ca⁽²⁺⁾-activated K⁽⁺⁾ channels[J].Proc Natl Acad Sci USA, 1999;96:10917-21

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