银杏叶提取物对实验性糖尿病大鼠脑组织的保护作用

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (8): 935-939.

银杏叶提取物对实验性糖尿病大鼠脑组织的保护作用

李剑敏, 李旭升, 陈国荣, 王芳, 毛孙忠¹, 吴步猛², 方周溪³, 杨开颜

温州医学院附属第一医院病理科, ¹生物化学教研室, ²实验动物中心, ³电镜室, 温州 325000, 浙江

收稿日期:2005-06-07 修回日期:2005-07-08 发布日期:2020-11-22
通讯作者: 杨开颜,女,副教授,研究方向:淋巴造血系统疾病病理。Tel:0577-88069545 E-mail:yky310@yahoo.com.cn
作者简介:李剑敏,男,副教授,研究方向:神经病理学。
基金资助:
浙江省教育厅资助课题基金项目(No20000674),温州市551人才基金专项资助课题(NoY2003A042)

Protective effects of extract of Ginkgo biloba on brain tissues in experimental diabetic rats

LI Jian-min, LI Xu-sheng, CHEN Guo-rong, WAN Fang, MAO Sun-zhong¹, WU Bu-meng², FANG Zhou-xi³, YANG Kai-yan

Department of Pathology, the First Affiliated Hospital, ¹Department of Biochemistry, ²Experimental Animal Center, ³Electro Microscopy Lab, Wenzhou Medical College, Wenzhou 325003, Zhejiang

Received:2005-06-07 Revised:2005-07-08 Published:2020-11-22

摘要/Abstract

摘要： 目的: 研究银杏叶提取物(extractofGinkgobiloba,EGB)对糖尿病大鼠脑的保护作用及其机制。方法: 选用雄性SD大鼠,随机分为糖尿病组、银杏叶组和正常对照组。应用链脲佐菌素(STZ)诱导制备大鼠糖尿病模型。银杏叶组腹腔注射银杏叶提取物注射液,其余两组给予同容积的生理盐水,用药5周后,测定脑组织内皮素(ET)、丙二醛(MDA)、NO产物NO2-NO3-的含量及超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的活性,以及血清中的血糖、胰岛素、ET水平,并作光镜及透射电镜观察。结果: 糖尿病脑组织光镜下可见脑水肿、脑软化、神经细胞变性、白质脱髓鞘,透射电镜下可见神经元及神经胶质细胞内线粒体肿胀、嵴变短、神经纤维脱髓鞘、血脑屏障受损。EGB治疗后光镜、电镜下的病变明显减轻。与糖尿病组相比,银杏叶组大鼠血糖、血浆ET水平下降、胰岛素水平升高,脑组织内SOD活性明显升高,NOS活性、ET、MDA、NO2-NO3-的含量明显下降。结论: EGB可能通过抗脂质过氧化及降低NO、ET水平,对糖尿病大鼠脑组织产生保护作用。

关键词: 银杏叶提取物, 糖尿病, 脑, 一氧化氮, 脂质过氧化, 内皮素

Abstract: AIM: To study the protective effects of the extract of Ginkgo biloba (EGB) on brain tissues in experimental diabetic rats and explore its possible mechanisms.METHODS: Thirty male Sprague-Dauley rats were divided into three groups:normal control group, diabetic group and EGB-treated group.Strepozotocin were injected intraperitoneally on the later two groups to induce diabetes, EGB-treated group was injected intraperitoneally with EGB, and the others were treated with normal saline at the same volume.After five weeks, the content of endothelin (ET), malondial dehyde (MDA), NO2-NO3-, and the activity of superoxide dismutase (SOD), nitric oxide synthase (NOS) were determined in brain homogenate, and the level of blood glucose, insulin and ET were measured respectively.In addition, the morphologic changes of the brain tissue were studied by light microscopy and electron microscopy, respectively.RESULTS: In diabetic group, there were degeneration of neuron, brain edema, softened focus and demyelination in the white matter of brain by light microcopy.There were expansion of mitochondria of neuron and gliocyte, the shortened of crista, demyelination of neurofiber and injury of bloodbrain-barrier by the electron microscopy.After treated with EGB, the pathological changes decreased in brain under light microcopy and electron microcopy.Compared with diabetic rats, the activity of SOD and the level of serum insulin increased, while the level of blood ET, the activity of NOS, the content of ET, MDA, NO2-NO3-decreased in EGB-treated rats (P <0.05).CONCLUSION: EGB can protect diabetic brain by anti-lipid peroxidation and decrease the level of NO and ET.

Key words: extract of Ginkgo biloba, diabetes mellitus, experimental, brain, nitric oxide, lipid peroxiedation, endothelin

中图分类号:

R587.1

李剑敏, 李旭升, 陈国荣, 王芳, 毛孙忠, 吴步猛, 方周溪, 杨开颜. 银杏叶提取物对实验性糖尿病大鼠脑组织的保护作用[J]. 中国临床药理学与治疗学, 2005, 10(8): 935-939.

LI Jian-min, LI Xu-sheng, CHEN Guo-rong, WAN Fang, MAO Sun-zhong, WU Bu-meng, FANG Zhou-xi, YANG Kai-yan. Protective effects of extract of Ginkgo biloba on brain tissues in experimental diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(8): 935-939.

参考文献

1 AK G, Buyukberber S, Sevinc A, Turk HM, AtesM, Sari R, et al.The ralation between plasma endothelin-1 levels and metabolic control, risk factors treatment modalities and diabetic microangiopathy in palients with Type 2 diabetes mellitus[J].J Diabetes Complications, 2001;15:150-7
2 Ciarla MV, Bocciarelli A, Di Gregorio S, Tordi A, Cotroneo P, Marra G, et al.Autoantibodied and endothelial dysfunction in well controlled uncomplication insulin-dependent diabetes mellitus palients[J].Atherosclerosis, 2001;158:241-6
3 Graier WF, Simecer S, Kurovetz WZ, Kostner GM.High Dglucose-induced charges in endothelial Ca²⁺EDRF signalding are due to generation of superoxide anions[J].Diabetes, 1996; 45:1386-95
4 Schror K.Blood vessel wall interactions in diabetes[J].Diabetes, 1997;46:5115-8
5 Vlassara H.Recent progress in advanced gly cation end products and diabetic complications[J].Diabetes, 1997;46:519-25
6 Lerman A, Holmes DRJr, Bell MR, Garratt KN, Nishimura RA, Burnett JCJr.Endothelin in coronary endothelial dysfunction and early atherosclerosis in humans[J].Circulation, 1995; 92:2426-31
7 Greenberg DA, Chan J, Sampson HA.Endothelins and the nervous system[J].Neurology, 1992;42:25-31
8 Faraci FM, Brian JEJr.Nitric oxide and cerebral circultion[J]. Stroke, 1994;25:692-703
9 Dawson TM, Dawson VL, Snyder SH.A novel neuronal messenger molecule in brain:the free radical, nitric oxide[J].Ann Neurol, 1992;32:297-311
10 BrownGC, Cooper CE.Nanomolar concentrations of nitric oxide reversibly inhibit synaptosomal respiration by competing with oxygen at cytochome oxidase[J].FEBS Lett, 1994;356:295-8
11 Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L.The nitric oxide-scavenging properties of Ginkgo biloba extract EGB761 [J].Biochem Biophy Res Commun, 1994;210:748-55
12 Oyama Y, Chilkahisa L, Ueha T, Kanemaru K, Noda K. Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide[J].Brain Res, 1996;712: 349-52
13 Sun BL, Xia ZL, Yang MF, Qiu PM.Changes of somatosensory evoked potential, serum and brain nitric oxide levels and effect of Ginkgo biloba extract following subarachnoid hemorrhage in rats[J].Chin J Pathophysiology, 2001;17:264-7

[1]	马言, 田高鹏, 石兴文, 孙婷, 谢晶晶, 甄东户. 探讨T2DM人群中25（OH）D与代谢相关性脂肪肝的相关性[J]. 中国临床药理学与治疗学, 2023, 28(9): 1018-1026.
[2]	许宜琪, 吴茜, 刘书, 刘帆, 邢春燕, 李勤, 何俊俊, 何春玲, 赵咏莉, 高家林. 雷公藤多苷治疗糖尿病肾脏病的临床疗效及抗炎抗纤维化分析[J]. 中国临床药理学与治疗学, 2023, 28(9): 1034-1042.
[3]	毛君, 荣玉, 李炎根, 熊登喜, 赵鹏, 查正江. 氟比洛芬酯在颅脑损伤患者镇痛治疗中的临床应用效果观察及安全性分析[J]. 中国临床药理学与治疗学, 2023, 28(9): 1056-1060.
[4]	马仙康, 杨丽霞, 崔阳阳, 米登海. 当归多糖通过抑制GRP78、p-PERK、p-Eif2α表达改善糖尿病KK-Ay小鼠肝内质网应激[J]. 中国临床药理学与治疗学, 2023, 28(8): 926-936.
[5]	王坤, 许佩佩, 周兰兰, 鲁晟. 人参皂苷Rg1调控Epac1/Rap1信号通路对缺血性脑卒中大鼠神经保护作用机制研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 721-727.
[6]	李士旭, 李林运, 王新, 李轲, 卞华. 银杏叶提取物通过miR-145/FOXO1轴对实验性肾功能衰竭大鼠肾脏损伤的影响[J]. 中国临床药理学与治疗学, 2023, 28(7): 728-735.
[7]	李坤, 李璐璐, 李楠楠, 胡卫红, 周建超. 成人肾移植术后血糖控制和CYP3A5基因多态性对他克莫司谷浓度的影响[J]. 中国临床药理学与治疗学, 2023, 28(7): 767-774.
[8]	李青华, 赵艳, 赵海港, 高朋飞, 徐炳欣. ABCB1 G2677T 基因多态性检测在缺血性脑卒中患者应用阿托伐他汀降脂治疗中的价值[J]. 中国临床药理学与治疗学, 2023, 28(6): 633-640.
[9]	秦文秀, 许军峰, 杨婷, 王坪霏. 丹参酮IIA治疗缺血性脑卒中后神经损伤的信号通路研究进展[J]. 中国临床药理学与治疗学, 2023, 28(6): 705-713.
[10]	侯瑞英, 郭莉阁, 焦伟杰, 杜蕾, 吴桂月, 赵旭. 消渴舒方对2型糖尿病模型大鼠血清炎症因子的影响及其机制研究[J]. 中国临床药理学与治疗学, 2023, 28(4): 377-382.
[11]	徐渭, 陈峰, 叶志军. 金丝桃苷调节RhoA/ROCK信号通路对创伤性脑损伤大鼠的影响[J]. 中国临床药理学与治疗学, 2023, 28(4): 383-390.
[12]	张雪娇, 刘洁婷, 李鲁新, 陈培剑, 丁明璐, 孙梦伟, 初彦辉, 张珍. 达格列净对1型糖尿病小鼠心肌损伤的影响及机制研究[J]. 中国临床药理学与治疗学, 2023, 28(3): 257-265.
[13]	吴志勇, 刘兵荣, 李彬, 汤睿. 红细胞分布宽度对急性缺血性卒中静脉溶栓早期神经功能改善不良的预测作用[J]. 中国临床药理学与治疗学, 2023, 28(3): 307-314.
[14]	白佳, 杨虹, 李玲玲, 张洋洋, 杨莹, 吕海宏. 维生素D通过线粒体/自噬途径影响糖尿病神经病变机制的研究[J]. 中国临床药理学与治疗学, 2023, 28(2): 214-219.
[15]	赵世峰, 宋向明, 姚建平. 新型胰高血糖素样肽-1受体/葡萄糖依赖性促胰岛素多肽受体双重激动剂——Tirzepatide[J]. 中国临床药理学与治疗学, 2023, 28(2): 220-227.