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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (8): 943-946.

• 研究原著 • 上一篇    下一篇

参附注射液对大鼠肾缺血再灌注损伤的防治作用

孙艳玲, 刘先义   

  1. 武汉大学人民医院麻醉科, 武汉 430060, 湖北
  • 收稿日期:2005-06-13 修回日期:2005-07-20 发布日期:2020-11-22
  • 通讯作者: 刘先义,男,主任医师,教授,硕士生导师,研究方向为器官保护。Tel:(0)13507117487 E-mail:whxyl2005@126.com
  • 作者简介:孙艳玲,女,主治医师,硕士研究生,主要从事麻醉与器官保护工作。Tel:(0)13872809376 E-mail:sysyl2005@126.com

Preventive and therapeutic effects of shenfu injection in rats with renal ischemia reperfusion

SUN Yan-ling, LIU Xian-yi   

  1. Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei, China
  • Received:2005-06-13 Revised:2005-07-20 Published:2020-11-22

摘要: 目的: 观察参附注射液(shenfuinjection,SF)对肾缺血再灌注损伤大鼠P38丝裂原活化蛋白激酶(P38MAPK)、肿瘤坏死因子-α(TNF-α)、细胞间粘附分子-1(ICAM-1)表达的影响,并探讨其肾脏保护作用的可能机理。方法: 动物随机分为假手术对照组、缺血再灌注组、参附预处理组3组。免疫组化法检测肾组织P38MAPK、ICAM-1蛋白含量,酶联免疫吸附实验(ELISA)检测肾组织和血浆TNF-α的表达。结果: 缺血再灌注组P38MAPK、ICAM-1表达和TNF-α含量明显高于假手术对照组(P<0.01)。与缺血再灌注组相比,参附组P38MAPK、ICAM-1表达和TNF-α含量明显降低(P<0.01)。结论: SF可能通过抑制P38MAPK的表达,从而下调TNF-α、ICAM-1的表达,发挥其肾脏保护作用。

关键词: 肾缺血再灌注, 参附注射液, P38 丝裂原活化蛋白激酶, 肿瘤坏死因子-α, 细胞间粘附分子-1

Abstract: AIM: To explore protective and therapeutic effects of the shenfu injection (SF)against renal ischemia reperfusionand and its possible mechanisms by studying the impact of SF on P38 mitogen activated protein kinase (P38MAPK), tumor necrosis factor alpha (TNF-α)and intercellular adhensive molecule-1 (ICAM-1).METHODS: 36male SD rats were randomly divided into control group, ischemia reperfusion (IR)group and SF group.Rats were subjected to left renal pedicle occlusion followed by reperfusion with contralateral nephrectomy.The expression of P38MAPK and ICAM-1 were evaluated by Imunohistochemistry.The expression of TNF-α in the kidney tissue and plasma were analyzed by enzymelinked immunoadsordent assay (ELISA).RESULTS: The expression of P38MAPK and ICAM-1, concentration of TNF-αin IR group were higher than that in the control group (P < 0.01).The expression of P38MAPK and ICAM-1, and concentration of TNF-αin SF group were lower than that in the IR group(P <0.01).CONCLUSION: The SF injection can downregulate the expression of TNF-αand ICAM-1 through inhibiting P38MAPK.SF has an important role in a renoprotective therapeutic regimen.

Key words: renal ischemia reperfusion, the shenfu injection, tumor necrosis factor-α, P38 mitogen activated protein kinase, intercellular adhensive molecule-1

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