黄芩苷对异丙肾上腺素诱导大鼠心肌缺血的治疗作用

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (9): 992-995.

黄芩苷对异丙肾上腺素诱导大鼠心肌缺血的治疗作用

吕健, 吴晓冬

东南大学基础医学院药理教研室, 南京 210009, 江苏

收稿日期:2005-07-09 修回日期:2005-08-16 发布日期:2020-11-22
通讯作者: 吴晓冬,男,博士,副教授,硕士生导师,主要从事心血管药理学研究。Tel:025-83918821 E-mail:robertwxd@yahoo.com.cn
作者简介:吕健,男,硕士研究生,研究方向:心血管药理。Tel:13770822112 E-mail:lujianjs@yahoo.com.cn

Protection effect of baicalin on isoproterenol induced myocardial ischemia in rats

LU Jian, WU Xiao-dong

Department of Pharmacology, the School of Basic Medical Science, Southeast University, Nanjing 210009, Jiangsu, China

Received:2005-07-09 Revised:2005-08-16 Published:2020-11-22

摘要/Abstract

摘要： 目的: 研究黄芩苷对异丙肾上腺素(isoproterenol, ISO)诱导的大鼠心肌缺血的治疗作用及其机理。方法: 将45 只SD 大鼠分为3 组, 每组15 只。正常对照组, 不给予任何处理;异丙肾上腺素(ISO)组, 皮下注射ISO 20 mg·kg^-1连续2 d;黄芩苷组给予ISO 后给予黄芩苷治疗。2 d 后测定其血流动力学指标。并测定血清中的乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)、肿瘤坏死因子-α(TNF-α)的含量。用免疫组化测定心肌组织中p38mapk 的表达。结果: 黄芩苷组较ISO 组能明显改善大鼠的心功能, 增强心肌的收缩和舒张功能, 血清中LDH、CK 的含量降低(P <0.01), 并能降低血清中TNF-α的含量(P <0.01)。心肌中p38mapk 的表达量显著降低。结论: 黄芩苷对异丙肾上腺素诱导的大鼠心肌缺血具有保护作用, 这一保护作用可能是通过下调p38mapk 表达从而抑制TNF-α的产生有关。

关键词: 黄芩苷, 异丙肾上腺素, 心肌缺血, p38mapk, 肿瘤坏死因子

Abstract: AIM: To study the protection effect of baicalin on isoproterenol (ISO)induced myocardial ischemia in rats and its mechanisms.METHODS: Fortyfive Sprague-Dawley rats were randomly divided into 3 groups.The rats in control group (n =15)received no treatment.The rats in ISO group (n =15)received two times of subcutaneous injection of ISO (20 mg·kg^-1), which was separated by a 24 hours-interval.The rats in baicalin group (n =15)received ISO as described above and received baicalin 50 mg·kg^-1 ip.simultaneously. Two days later the hemodynamic measurement was performed and the serum levels of LDH, CK and TNF-αwere detected.The expression of p38mapk in myocardium was detected by immunohistochemical technique.RESULTS: The heart function of the group treated with baicalin was improved significantly compared with that of control group.The levels of LDH, CK and TNF-αin baicalin group decreased significantly compared with those in control group and the expression of p38mapk in myocardium also decreased markedly.CONCLUSION: Baicalin has protection effect on isoproterenol induced myocardial ischemia in rats, and the effect maybe due to the decrease of TNF-αinduced by reducing the p38mapk expression.

Key words: baicalin, isoproterenol, myocardial ischemia, TNF-α, p38mapk

中图分类号:

R285.5

吕健, 吴晓冬. 黄芩苷对异丙肾上腺素诱导大鼠心肌缺血的治疗作用[J]. 中国临床药理学与治疗学, 2005, 10(9): 992-995.

LU Jian, WU Xiao-dong. Protection effect of baicalin on isoproterenol induced myocardial ischemia in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(9): 992-995.

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