AQP4 参与雌激素对单胺类神经递质的调节作用

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (11): 1256-1260.

AQP4 参与雌激素对单胺类神经递质的调节作用

李萍, 章静, 高琳, 范益, 孙秀兰, 丁建花, 胡刚

南京医科大学基础医学院神经药理研究室, 南京 210029 , 江苏

收稿日期:2006-10-10 修回日期:2006-11-10 出版日期:2006-11-26 发布日期:2020-11-06
通讯作者: 胡刚, 男, 博士, 教授, 博士生导师, 研究方向:神经退行性疾病的病因学与实验治疗学研究。Tel:025-86863108 E-mail:neuropha@njmu.edu.cn
作者简介:李萍, 女, 讲师。Tel:025-86863108 　E-mail:pli@njmu.edu.cn
基金资助:
国家自然科学基金(No30572172); 江苏省自然科学基金(No05KJB310075)

AQP4 deficiency alters regulative effects of estrogen on monoamine neurotransmitters

LI Ping, ZHANG Jing, GAO Lin, FAN Yi, SUN Xiu-lan, DING Jian-hua, HU Gang

Laboratory of Neuropharmacology , School of Basic Medical Science , Nanjing Medical University , Nanjing 210029 ,Jiangsu , China

Received:2006-10-10 Revised:2006-11-10 Online:2006-11-26 Published:2020-11-06

摘要/Abstract

摘要： 目的探讨AQP4 对雌激素调节神经递质作用的影响。方法应用雄性AQP4 基因敲除型CD1小鼠与野生型CD1 小鼠, 给予不同剂量雌激素后测定不同脑区中单胺类神经递质的含量。结果 AQP4基因敲除型雄性小鼠纹状体多巴胺(DA)及5-羟色胺(5-HT)、皮层去甲肾上腺素(NE)含量增加;海马NE 、下丘脑DA 水平下降。同时, 给予雌激素后, 野生型雄性CD1 小鼠纹状体DA 、5-HT 含量及海马5-HT 含量升高, 皮层DA 、5-HT 水平及下丘脑NE 、DA水平下降;而雌激素对AQP4 敲除型雄性CD1 小鼠单胺类递质无显著影响。结论 AQP4 敲除可改变雌激素对单胺类递质水平的调节作用, 对纹状体内DA水平的影响尤为显著。AQP4 参与了雌激素对单胺类神经递质的调节。

关键词: AQP4, 基因敲除, 单胺类递质, 雌激素

Abstract: AIM: To investigate whether aquaporin-4(AQP4)participates in the regulative action of estrogen on neurotransmitters (NT).METHODS: The monoamine neurotransmitters were determined by high performance liquid chromatography (HPLC)in different brain regions of male AQP4-knockout CD1 mice and wild-type CD1 mice after they were intraperitoneally injected with different doses of estradiol.RESULTS: The contents of DA and 5-HT in the striatum and NE in the cortex of AQP4-knockout CD1 were significantly increased , and the concentrations of NE in the hippocampus and DA in the hypothalamus were decreased.Treatment with estradiol increased the contents of DA and 5-HT in the striatum and that of 5-HT in the hippocampus , decreased the levels of DA and 5-HT in the cerebrum, and also reduced the concentrations of DA and NE in the hypothalamus in wildtype mice.However , these changes were not found in AQP4-knockout mice.CONCLUSION: The deficiency of AQP4 in mice alters the regulative effects of estrogen on monoamine neurotransmitters , indicating that AQP4 participates in regulating metabolism of neurotransmitters.

Key words: aquaporin 4, knockout, monoamine neurotransmitter, estrogen

中图分类号:

R966

李萍, 章静, 高琳, 范益, 孙秀兰, 丁建花, 胡刚. AQP4 参与雌激素对单胺类神经递质的调节作用[J]. 中国临床药理学与治疗学, 2006, 11(11): 1256-1260.

LI Ping, ZHANG Jing, GAO Lin, FAN Yi, SUN Xiu-lan, DING Jian-hua, HU Gang. AQP4 deficiency alters regulative effects of estrogen on monoamine neurotransmitters[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(11): 1256-1260.

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