白藜芦醇对2 型糖尿病患者外周血单个核细胞DNA 损伤的体外修复

摘要/Abstract

摘要： 目的观察2 型糖尿病( T2DM) 患者氧化抗氧化状态、外周血单个核细胞( PBMCs) DNA 损伤情况及白藜芦醇的体外修复作用。方法采用单细胞凝胶电泳技术检测39 例T2DM 血管并发症、29 例T2DM 无并发症患者PBMCs DNA 损伤情况( 用彗星率表示) , 并与26 名体检健康者对照比较。检测受试者血浆总抗氧化能力( TAC) 、丙二醛( MDA) 及巯基( SH) 含量。观察白藜芦醇在体外对T2DM 患者PBMCs DNA 损伤的修复作用。结果 T2DM 无并发症组PBMCs 彗星率及血浆MDA 含量明显高于健康对照组( P<0.01) , 血浆SH 含量、TAC 明显低于健康对照组( P<0.01) ;T2DM 血管并发症组彗星率及MDA 含量高于无并发症组( 或P<0.05) , TAC 低于无并发症组( P<0.05) 。T2DM 无并发症组及血管并发症组患者彗星率与MDA 含量呈正相关( r 分别为0.446 、0.504, P<0.05) , 与TAC 呈负相关( r 分别为-0.418 、-0.399 ;P<0.05) 。体外修复显示:10 μmol·L^-1白藜芦醇作用4 h 显著降低了T2DM 患者PBMCs 彗星率( P<0.01) 。结论 T2DM 患者体内存在氧化抗氧化失衡、DNA 氧化损伤, 血管并发症组重于无并发症组。氧化应激可能参与了T2DM 患者血管并发症的发生发展。抗氧化剂白藜芦醇在体外减轻T2DM 患者PBMCs DNA 损伤。

关键词: 2 型糖尿病, 氧化应激, DNA 损伤, 白藜芦醇

Abstract: AIM: To explore the oxidant-antioxidant status and levels of mononuclear cells ( PBMCs) oxdative DNA damage in patients with type 2 diabetes mellitus( T2DM) , and to value the effect of resveratrol on peripheral blood mononuclear cells oxidative DNA damage in T2DM patients in vitro.METHODS: The DNA damages in PBMCs of 39 T2DM patients with vascular complication,and 29 T2DM patients without complication were detected by single cell gel electrophoresis ( the comet percentage).The levels of plasma total antioxidative capacity(TAC) , the contents of plasma malondialdehyde (MDA) and plasma thiol grouP( SH) were measured.The effect of resveratrol on DNA damage of T2DM patients were investigated in vitro.In addition, 26 healthy subjects were studied as a control grouP.RESULTS: The comet percentage of PBMCs and the contents of plasma MDA in T2DM patients without complication were all significantly higher than that in healthy controls ( P<0.01).The levels of plasma TAC and the contents of plasma thiol grouPin T2DM patients without complication were all remarkably lower than that in healthy controls ( all P<0.01).The comet percentage of PBMCs and the contents of plasma MDA in T2DM patients without complication were significantly lower than that in T2DM patients with vascular complication( P<0.05) , and the levels of plasma TAC in T2DM patients without complication were significantly higher than that in T2DM patients with vascular complication( P<0.05).There were positively correlations between the comet percentage of PBMCs and the contents of plasma MDA in T2DM patients without complication and T2DM patients with vascular complication( r =0.446, P<0.05 ;r =0.504, P<0.01) ;and there were nega-tively correlations between the comet percentage of PBMCs and the contents of plasma TAC in the two groups( r =-0.418, P<0.05 ;r =-0.399, P<0.05).The comet percentage of PBMCs of patients were reduced by 10μmol·L^-1 resveratrol at 4 hours in vitro ( P<0.05).CONCLUSION: There are oxidant-antioxidant unbalance and oxidative DNA damage in T2DM patients, particularly in the patients with vascular complication.Oxidative stress is probably involved in pathogenesis of diabetic vascular complication.Resveratrol can reduce the oxdative DNA damage of PBMCs in patients with T2DM in vitro.Antioxidants supplementation may benefit the prevention of diabetic vascular complication.

Key words: type 2 diabetes mellitus, oxidative stress, DNA damage, resveratrol

中图分类号:

R965.2
R966

姜忠, 汤旭磊. 白藜芦醇对2 型糖尿病患者外周血单个核细胞DNA 损伤的体外修复[J]. 中国临床药理学与治疗学, 2006, 11(11): 1285-1290.

JIANG Zhong, TANG Xu-lei. Effect of resveratrol on peripheral blood mononuclear cells oxidative DNA damage in type 2 diabetes mellitus patients in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(11): 1285-1290.

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