前列腺素DP受体缺乏小鼠的睡眠-觉醒特征

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (11): 1296-1300.

前列腺素DP受体缺乏小鼠的睡眠-觉醒特征

马张庆, 洪宗元

皖南医学院定量药理研究室, 芜湖 241000, 安徽

收稿日期:2006-10-11 接受日期:2006-11-06 出版日期:2006-11-26 发布日期:2020-11-06
基金资助:
Supported by the Educational Foundation of Anhui Province ( No2006KJ353B) and the research foundation of Wannan Medical College for Ph.D.

Characteristics of sleep-wake cycles in mice lacking prostanoid DPreceptors

MA Zhang-qing, HONG Zong-yuan

Lab.of Quantitative Pharmacology, Wannan Medical College, Wuhu241001, Anhui, China

Received:2006-10-11 Accepted:2006-11-06 Online:2006-11-26 Published:2020-11-06
Contact: HONG Zong-yuan,male, Ph.D., professor, research fields:neuropharmacology and quantitative Pharmacology.Tel:0553-5875499 　E-mail:zyhongwnmc@yahoo.com.cn
About author:Ma Zhang-qing, male, postgraduate.Tel:13855326057 E-mail:ma929 @56.com

摘要/Abstract

摘要： 目的探讨前列腺素DP受体( DPR) 对小鼠睡眠-觉醒调节的影响。方法在苯巴比妥麻醉下, 于DPR 基因敲除(KO) 小鼠及其野生型(WT) 小鼠大脑皮层及颈部肌肉分别植入脑电( Electroencephalogram,EEG) 和肌电( Electromyogram, EMG) 电极, 用EEG EMG 睡眠记录系统于20 ∶00 时开始连续记录24 小时两种小鼠的脑电和肌电波, 并用SLEEPSIGN软件进行分析。结果两种小鼠表现出相同的睡眠-觉醒节律, 且明时( 8 ∶00 -20 ∶00) 及暗时( 20 ∶00 -8 ∶00) 时相内两种小鼠的非快动眼( NREM) 睡眠和快动眼( REM) 睡眠总量无差异。但与WT 小鼠相比, DPR-KO 小鼠明时内的觉醒频率显著增高,NREM 睡眠的平均时程显著缩短;且DPR-KO 小鼠睡眠呈现低活性的θ波和高活性的δ波。结论 DPR在介导前列腺素D₂诱导的睡眠中起着关键性调节作用, 缺乏DPR 将导致小鼠呈现低强度片段化的NREM 睡眠和高警戒状态的REM 睡眠。

关键词: 前列腺素D2, 前列腺素DP受体, 睡眠-觉醒, 非快动眼睡眠, 快动眼睡眠, 小鼠

Abstract: AIM: To investigate the effect of prostanoid DPreceptors ( DPR) on sleep-wake regulation in mice.METHODS: Under pentobarbital anesthesia,mice were chronically implanted with electroencephalogram( EEG) and electromyogram ( EMG) electrodes for polysomnographic recordings.The spontaneous sleep-wake cycles were monitored continuously by EEG/EMG recording system for 24 h beginning at 8 ∶; 00 P.m.and analyzed by SLEEPSIGN software in DPR knock out ( KO)and wild type (WT) mice.RESULTS: DPR-KO mice exhibited a similar circadian rhythm of sleep-wake cycles to WT mice.The amounts of rapid eye movement ( REM)sleePor non-REM ( NREM) sleePduring both the light and dark periods were identical between the DPR-KO and WT mice.Whereas, an increase in the episode number of wakefulness and a shortage in the duration of NREM sleePwere found in DPR-KO mice during the light period compared with WT mice.Moreover, DPR-KO mice showed lower activity in delta-wave component in NREM sleePand higher activity in theta-wave component in REM sleePthan WT mice.CONCLUSION: DPR plays a crucial role in mediating the prostaglandin D2-induced sleeP.Deficiency of DPR results in the low intensity and fragmented diurnal NREM sleePand the high vigilance REM sleep,with the normal circadian rhythm of sleePin mice.

Key words: prostaglandin D2, DPreceptor, sleepwake cycle, rapid eye movement sleep, non-rapid eye movement sleep, mice

中图分类号:

R965

马张庆, 洪宗元. 前列腺素DP受体缺乏小鼠的睡眠-觉醒特征[J]. 中国临床药理学与治疗学, 2006, 11(11): 1296-1300.

MA Zhang-qing, HONG Zong-yuan. Characteristics of sleep-wake cycles in mice lacking prostanoid DPreceptors[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(11): 1296-1300.

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