红花对大鼠肾间质纤维化和肾功能的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (3): 282-285.

红花对大鼠肾间质纤维化和肾功能的影响

唐蓉, 杜胜华

广东医学院附属医院肾病内科,湛江 524001,广东

收稿日期:2005-11-25 修回日期:2006-02-06 出版日期:2006-03-26 发布日期:2020-12-04
作者简介:唐蓉,女,本科毕业,副主任医师,研究方向:腹膜透析。Tel:13652886969 E-mail:gyhtrgh@163.com

Effect of safflower on renal interstitial fibrosis and renal function in rats

TANG-Rong, DU Shen-hua

Department of Nephrology,Affiliated Hospital of Guangdong Medical college,Zhangjiang 524001,Guangdong,China

Received:2005-11-25 Revised:2006-02-06 Online:2006-03-26 Published:2020-12-04

摘要/Abstract

摘要： 目的探讨红花对左肾静脉结扎大鼠动物模型中肾功能及肾脏病理的影响,旨在了解红花在延缓肾间质纤维化中的作用。方法 48只大鼠随机分为6组,正常对照、假手术组、手术对照、手术未治疗组、红花提取液治疗组、依那普利治疗组。以菊粉清除率检测肾小球滤过率(GFR)。常规病理染色及免疫组织化学染色后,采用Motic医学图象分析系统分析肾脏病理改变。结果红花提取液治疗组、依那普利治疗组经治疗,GFR有所恢复,与手术未治疗组组比较,P<0.05;病理结果显示,手术未治疗组和手术治疗组均可见灶性肾小管萎缩,间质纤维组织增生,但前两者较重。CK-18免疫组化染色显示,手术治疗组阳性染色的比值较对照组及手术治疗组高(P<0.05);测肾小管直径结果显示,红花及依那普利治疗组与手术治疗组组相比,P<0.05。FN表达分析结果显示,红花提取液治疗组、依那普利治疗组较手术对照组和手术非治疗组低(P<0.05)。结论红花通过阻止肾间质纤维化,可延缓慢性肾脏疾病的进程。

关键词: 红花, 肾间质纤维化, 大鼠, 肾功能减退

Abstract: AIM: To research the effect of safflower in renal intertistial fibrosis by GFR and renal biopsypathology with Sprague-Dnwley(SD) rat model.METHODS: All rats were distributed into 6 groups.GrouPA was normal control,grouPB received sham operation,grouPC was vehicle control,grouPD received vehicle,grouPE received safflower(10 g·kg^-1 body wt·d^-1),and grouPFreceived enalapril (12.5 mg·kg^-1 body wt·d^-1).Rats underwent reversible left renal vein obstruction for 6 d,after which the obstruction was relieved.Renal function was determined by measuring GFR with inulin clearances.Both renal tubular epithelial cells(RTECs) and fibronection deposition for renal interstitial fibrosis were identified by immunohistochemistry.The positive and negative areas of per slice was determined by a motic medical 6.0 graphical analytical system.Atrophic tubules were determined by measuring the tubule diameter.10 fields per slice were analyzed at×400 magnification.RESULTS:Return of glomerular filtration rate(GFR) was seen in grouPE and gouPF,which was significantly better than grouPD(P<0.05).Atrophic tubules and fibronection deposition were found except in grouPA and groupB,but grouPD was worse significantly than grouPE and grouPF(P<0.05).The proportionalit of positive areas of RTECs recovered more significantly in grouPE and grouPF than grouPD(P<0.05).CONCLUSION: Safflowei can return renal function and postpone the development of chronic kidney disease by preventing renal interstitial fibrosis.

Key words: safflower, renal interstitial fibrosis, rat, renal failure

中图分类号:

R392

唐蓉, 杜胜华. 红花对大鼠肾间质纤维化和肾功能的影响[J]. 中国临床药理学与治疗学, 2006, 11(3): 282-285.

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