高糖环境早期对血管内皮细胞的作用之商榷

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (3): 309-313.

高糖环境早期对血管内皮细胞的作用之商榷

薛薇, 李卫东, 滕慧玲, 林志彬

北京大学医学部基础医学院药理系,北京 100083

收稿日期:2005-12-07 修回日期:2006-03-01 出版日期:2006-03-26 发布日期:2020-12-04
通讯作者: 李卫东,女,博士,副教授,硕士生导师,研究方向:免疫药理。Tel:010-82802798 E-mail:lwdpharma@126.com
作者简介:薛薇,女,在读硕士。Tel:010-82801473 E-mail:roserebel@126.com

Discussion about effect of high glucose environ on vascular endothelial cells in earlier period

XUE Wei, LI Wei-dong, TENG Hui-ling, LIN Zhi-bin

Department of Pharmacology,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100083,China

Received:2005-12-07 Revised:2006-03-01 Online:2006-03-26 Published:2020-12-04

摘要/Abstract

摘要： 目的探讨高糖环境在48h内对人脐静脉血管内皮细胞(HUVEC)的增殖以及粘附分子表达的影响,并观察在炎性刺激下高糖作用的改变。方法用不同浓度的葡萄糖作用于HUVEC,用噻唑蓝法(MTT法)检测48h内高糖对细胞增殖的影响。采用流式细胞术及细胞酶联免疫吸附法(cellELISA)测定高糖作用下HUVEC表达的细胞间粘附分子(ICAM-1)的水平。脂多糖(LPS)与高糖联合刺激HUVEC后,比较其与只用LPS刺激情况下的ICAM-1的表达之间的差异。结果 25、45mmol·L^-1的葡萄糖在48h内无抑制HUVEC增殖的作用,对其表达粘附分子的水平也无显著影响。但在LPS刺激条件下,高糖组HUVEC的增殖明显被抑制,ICAM-1水平也较正常糖浓度组显著增加。结论在短期内高糖对HUVEC的增殖及粘附分子的表达无明显影响,而在炎症刺激存在下,高糖显著抑制其增殖、增加粘附分子的表达。

关键词: 高糖, 人脐静脉内皮细胞, 增殖, 粘附分子

Abstract: AIM: To discuss the influence of high glucose on the proliferation and ICAM-1 expression of human umbilical vein endothelial cells(HUVEC) within 48h and to observe the altered effect of high glucose when high glucose and inflammatory stimulator exist at the same time.METHODS: HUVEC were cultured with different concentrations of glucose.The proliferation of HUVEC induced by high glucose was detected by MTT assay.The ICAM-1expression of HUVEC induced by high glucose was assayed by flow cytometry and cell ELISA.The difference of ICAM-1 expression between induced by LPS and by LPS together with high glucose was compared.RESULTS: 25 、45 mmol·L^-1 glucose did not inhibit the proliferation of HUVEC within 48 h,and they also had no effect on the expression of ICAM-1.However,when stimulated HUVEC with LPS,the proliferation of HUVEC was extremely inhibited in high glucose groups,and the expression of ICAM-1 increased significantly in those groups.CONCLUSION: In short time,high glucose has no effect on the proliferation and ICAM-1 expression of HUVEC,but when inflammatory stimulation exists,high glucose can inhibit the proliferation and increase the expression of adhesion molecules.

Key words: high glucose, HUVEC, proliferation, adhesion molecule

中图分类号:

R965.1

薛薇, 李卫东, 滕慧玲, 林志彬. 高糖环境早期对血管内皮细胞的作用之商榷[J]. 中国临床药理学与治疗学, 2006, 11(3): 309-313.

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