氯离子通道阻断剂对大鼠低氧性肺动脉高压的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (6): 677-680.

氯离子通道阻断剂对大鼠低氧性肺动脉高压的影响

莫碧文, 曾锦荣, 李国坚, 黄文新, 于方¹, 王绩英, 王昌明

桂林医学院附属医院呼吸内科, 桂林 541001, 广西;
¹华中科技大学同济医学院同济医院, 武汉 430030, 湖北

收稿日期:2006-03-12 修回日期:2006-04-03 出版日期:2006-06-26 发布日期:2020-12-04
通讯作者: 王昌明,男,博士,教授,研究方向:肺动脉高压。Tel:0773-2823748 E-mail:wangcm@public.glptt.gx.cn
作者简介:莫碧文,女,博士,副教授,研究方向:肺动脉高压。Tel:0773-3937489 E-mail:mobiwen2002@sohu.com
基金资助:
国家自然科学基金资助项目(No30260040);广西“新世纪十百千人才工程” 人选专项资金资助项目(No2001211) 及广西区教育厅资助项目(No 桂教科研[2002] 316)

Effects of chloride channel blocker on hypoxia induced rat pulmonary hypertension

MO Bi-wen, ZENG Jin-rong¹, LI Guo-jian, HUANG Wen-xin, YU Fang¹, WANG Ji-ying, WANG Chang-ming

Department of Respiratory Medicine, Hospital affiliated Guilin Medical College, Guilin 541001, Guangxi, China;
¹Tongji hospital affiliated Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China

Received:2006-03-12 Revised:2006-04-03 Online:2006-06-26 Published:2020-12-04

摘要/Abstract

摘要： 目的: 探讨氯离子通道阻断剂三苯氧胺(tamoxifen, TAM) 对大鼠低氧性肺动脉高压的治疗效果。方法: 雄性Wistar 大鼠96 只, 随机分为:(1) 治疗组(缺氧+三苯氧胺, H Q):大鼠在常压缺氧(10%, 每日8 h) 箱内饲养, 缺氧前3 d 开始灌胃三苯氧胺0.4 mg·kg^-1·d^-1, 每日1 次;(2) 缺氧组(H组):每日给予相等量的生理盐水, 余同治疗组;(3)正常对照组(C 组)。测定各组大鼠d 4、7、14、21 右心室/(左心室+室间隔) 厚度, 多导生理记录仪记录肺动脉平均压(mPAP), 图像分析仪测定血管管壁面积占管总面积的百分比(WA/TA) 和血管管腔面积占管总面积的百分比(VA/TA)。结果: 缺氧组d 7mPAP 开始升高, d 21 达高峰, 治疗组同时间点明显低于缺氧组;缺氧组RV/(LV+S) d 14 开始上升,d 21 达高峰, 治疗组同时间点明显低于缺氧组;缺氧组d 21 的血管管壁面积占管总面积的百分比(WA/TA) 显著高于d 21 治疗组(P<0.05)。而缺氧组d 21 的血管管腔面积占管总面积的百分比(VA/TA) 显著低于d 21 治疗组(P<0.05)。结论: 氯离子通道阻断剂三苯氧胺可降低缺氧所致大鼠肺动脉压升高、改善右心室肥厚及肺小动脉增厚, 对低氧性肺动脉高压有一定的治疗作用。

关键词: 三苯氧胺, 肺动脉高压, 低氧

Abstract: AIM: To investigate the therapeutic effect of chloride channel blocker tamoxifen on hypoxia pulmonary hypertension in rats.Methods: Sixty male Wistar rats were randomly divided intotherapeutic group (H/Q group), hypoxia group(H group), normal control group(C group).Mean pulmonary artery pressure(mPAP), the ratio of the weight of right ventricle to that of left ventricle plus septum[RV(LV+S)], and the ratio of the pulmonary arteriole wall area to that of vascular total area(WA/TA), were measured at 4, 7, 14, 21 days.Results: The mPAP began to increase from 7 days, and reached peak at 21 days in hypoxia group, however it is obviously lower in therapeutic group at same time.The ratio of RV/(LV+S) began to increase from 14 days, and reached peak at 21 days in hypoxia group, however it is obviously lower in therapeutic group at same time.The ratio of WA/TA at 21 days in hypoxia group was significantly higher than therapeutic group, respectively(P<0.05).The ratio of VA/TA at 21 days in hypoxia group was significantly lower than therapeutic group, respectively (P<0.05).Conclusion: The data suggested Tamoxifen could reduce hypoxia-induced increased pulmonary artery pressure, inhibit hypoxia-induced hypertrophic right ventricle and pulmonary small artery thickness.Tamoxifen may be used in the treatment of hypoxia induced pulmonary hypertension.

Key words: tamoxifen, hypoxia pulmonary hypertension, hypoxia

中图分类号:

R965.1

莫碧文, 曾锦荣, 李国坚, 黄文新, 于方, 王绩英, 王昌明. 氯离子通道阻断剂对大鼠低氧性肺动脉高压的影响[J]. 中国临床药理学与治疗学, 2006, 11(6): 677-680.

MO Bi-wen, ZENG Jin-rong, LI Guo-jian, HUANG Wen-xin, YU Fang, WANG Ji-ying, WANG Chang-ming. Effects of chloride channel blocker on hypoxia induced rat pulmonary hypertension[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(6): 677-680.

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