D-半乳糖对大鼠下丘脑弓状核神经元超微结构的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (6): 709-711.

D-半乳糖对大鼠下丘脑弓状核神经元超微结构的影响

罗红梅, 崔燎¹, 吴铁¹

广东医学院生理科学实验室, ¹广东医学院药理教研室, 湛江 524023, 广东

收稿日期:2006-03-15 修回日期:2006-06-10 出版日期:2006-06-26 发布日期:2020-12-04
作者简介:罗红梅,硕士,助教,从事骨药理学研究。Tel:0759-2388424 E-mail:lhmjyb@yahoo.com.cn

Effects of D-galactose on ultrastructure of rats'hypothalamic arcuate nucleus

LUO Hong-mei, CUI Liao¹, WU Tie¹

Laboratory of Physiological Science, ¹Department of Pharmacology, Guangdong Medical Gollege, Zhanjiang 524023, Guangdong, China

Received:2006-03-15 Revised:2006-06-10 Online:2006-06-26 Published:2020-12-04

摘要/Abstract

摘要： 目的: 观察D-半乳糖对大鼠下丘脑弓状核超微结构的影响。方法: 12 只6 月龄大鼠随机分成2组, 对照组和D-半乳糖组, 对照组每天皮下注射生理盐水, D-半乳糖组每天皮下注射D-半乳糖, 剂量为100 mg·kg^-1, 连续给药3 个月后, 在全麻下, 心脏放血处死大鼠, 立即从左心室灌注2.0%~2.5%多聚甲醛-戊二醛的固定液50 mg·kg^-1, 并完整取出脑, 放入2.5%戊二醛溶液中后固定, 根据脑定位图谱取出大鼠弓状核, 最后将取出组织块制成超薄切片在电镜下进行观察。结果: 与对照组大鼠相比,D-半乳糖组大鼠弓状核神经元表现出衰老样变化,部分神经元细胞胞浆内细胞器明显减少, 胞体减小,脂褐素增多, 并观察到典型的胞核染色质边聚的凋亡神经元和轴突明显萎缩的老化神经元, 而对照组大鼠神经元没有观察到明显的结构变化, 胞浆中含有丰富的细胞器。结论: D-半乳糖可导致大鼠下丘脑弓状核发生衰老样变化, 神经元发生萎缩和凋亡。

关键词: D-半乳糖, 弓状核, 电镜技术

Abstract: AIM: To investigate the effects of D-galactose on ultrastructure of rats'hypothalamic arcuate nucleus.Methods: 12 rats at age of 6 months were divided into two groups at random, the control and D-galactose group.Rats of control group were treated with saline solution by sc, and rats of D-galactose group were treated with D-galactose by sc at dose of 100 mg·kg^-1. Three months later the rats were killed by exsanguinating from heart.After being infused into the left ventricle with 2%-2.5% polyformaldehyde at the dose of 50 mg·kg^-1, the brains were taken and immerged in 2.5% polyformaldehyde, then the arcuate nucleus was taken according to the atlas of brain, the tissues taken were made into ultrathin sections to be observed under electron microscope.Results: comparing torats in control group, neurons of the arcuate nucleus of rats in D-galactose group appeared aging, the number of organelle in plasma decreased, but the number of lipochromesome in plasma increased significantly, and the size of neurons decreased also.Furthermore the apoptosis neuron was observed, the chromosome of which congregated around the nucleus'membrane, the typical aging neuron was also observed, the neuraxon of which was atrophying.But there were no obvious changes observed in neurons of the arcuate nucleus of rats in control group, plenty of organelles were observed under electron microscope clearly.Conclusion: Dgalactose can cause neurons of rats'arcuate nucleus aging, the neurons appeared atrophying and apoptosis.

Key words: D-galactose, arcuate nucleus, electron microscopic technique

中图分类号:

R965.1

罗红梅, 崔燎, 吴铁. D-半乳糖对大鼠下丘脑弓状核神经元超微结构的影响[J]. 中国临床药理学与治疗学, 2006, 11(6): 709-711.

LUO Hong-mei, CUI Liao, WU Tie. Effects of D-galactose on ultrastructure of rats'hypothalamic arcuate nucleus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(6): 709-711.

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