土贝母苷甲对人红白血病细胞K562的诱导分化作用

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (7): 743-747.

土贝母苷甲对人红白血病细胞K562的诱导分化作用

刘姬艳, 高营, 于立坚, 马润娣

广东海洋大学广东省海洋药物研究与开发重点实验室, 湛江 524025 , 广东

收稿日期:2006-03-07 修回日期:2006-05-30 出版日期:2006-07-26 发布日期:2020-10-30
作者简介:刘姬艳, 女, 硕士, 研究方向:细胞生物学。作者现工作单位, 杭州师范学院科学学院。
基金资助:
广东省自然科学基金资助项目(No011809)

Differentiation-inducing activity of tubeimoside I in human erythroleukemicK562 cells

LIU Ji-yan, GAO Ying, YU Li-jian, MA Run-di

Guangdong Provincial Key Laboratory of Marine Materia Medica , Guangdong Ocean University , Zhanjiang 524025 ,Guangdong , China

Received:2006-03-07 Revised:2006-05-30 Online:2006-07-26 Published:2020-10-30

摘要/Abstract

摘要： 目的研究土贝母苷甲对人红白血病细胞K562 分化的影响。方法采用台盼蓝拒染法检测土贝母苷甲对K562 细胞增殖的影响;采用细胞形态学、细胞化学方法检测土贝母苷甲对K562 细胞分化的影响;采用Western blotting 法检测土贝母苷甲对K562 细胞早期原癌基因c-myc 、c-fos 表达的影响。结果土贝母苷甲下调K562 细胞c-myc 的表达, 上调c-fos 的表达, 诱导K562 细胞产生血红蛋白, 诱导K562 细胞向成熟红系细胞方向分化。结论土贝母苷甲对人红白血病细胞K562 具有诱导分化作用。

关键词: 土贝母苷甲, 诱导分化, K562细胞

Abstract: AIM: To examine the differentiation-inducing effect of tubeimoside I on human erythroleukemic K562 cells.METHODS: The growth inhibitory activity of tubeimoside I was evaluated with K562 cells.Trypan blue exclusion , morphological observation , cytochemistry were used to observe the features of erythroid , granulocytic,and megakaryocytic lineages differentiated from K562 cells.Using Western blot analysis , we studied alterations in c-myc and c-fos expression accompanying tubeimoside I-induced differentiation of K562 cells.RESULTS: Tubeimoside I inhibited the proliferation K562 cells and induced the differentiation of K562 cells into the erythrocyts.It could down-regulate c-myc and up-regulate c-fos ,and the steps were found to be the early events of K562 cell differentiation mediated by tubeimoside I.CONCLUSION: Tubeimoside I can induce the differentiation of K562 cells into the erythrocytes , and down-regulation of c-myc and up-regulation of c-fos which are the early responses of K562 cells to the induction of differentiation mediated by tubeimoside I.

Key words: tubeimoside I, differentiation-inducing activity, K562 cells

中图分类号:

刘姬艳, 高营, 于立坚, 马润娣. 土贝母苷甲对人红白血病细胞K562的诱导分化作用[J]. 中国临床药理学与治疗学, 2006, 11(7): 743-747.

LIU Ji-yan, GAO Ying, YU Li-jian, MA Run-di. Differentiation-inducing activity of tubeimoside I in human erythroleukemicK562 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 743-747.

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