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中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (8): 940-942.

• 研究原著 • 上一篇    下一篇

甘草提取物对酪氨酸酶活性的抑制作用

沈钧, 何莉萍, 张瑞斌   

  1. 天津医科大学公共卫生学院卫生化学教研室, 天津 300070
  • 收稿日期:2006-05-18 修回日期:2006-06-16 出版日期:2006-08-26 发布日期:2020-11-05
  • 作者简介:沈钧, 男, 讲师, 本科, 研究方向:分析测定。Tel:022-23542640  E-mail:shenjun@tijmu.edu.cn

Inhibitory effect of licoric extracts on activity of tyrosinase

SHEN Jun, HE Li-ping, ZHANG Rui-bin   

  1. Department of Sanitary Chemistry , School of Public Health, Tianjin Medical University , Tianjin 300070 , China
  • Received:2006-05-18 Revised:2006-06-16 Online:2006-08-26 Published:2020-11-05

摘要: 目的 研究甘草提取物对酪氨酸酶活性的抑制作用。方法 用50%(V/V) 乙醇提取甘草中的有效成分, 分别以L-酪氨酸、邻苯二酚为底物, 在475 、390 nm 处测定溶液的吸光度, 观察甘草提取物对单酚酶、双酚酶活性的影响。结果 50%(V/V) 乙醇甘草提取物对酪氨酸酶有明显的抑制作用(IC50分别为1.94 、2.94 mg·ml-1) 。结论 甘草提取物对酪氨酸酶单酚酶、双酚酶的活性均有抑制作用;以邻苯二酚为底物的双酚酶活性测定体系可较好地应用于酪氨酸酶活性的研究。

关键词: 酪氨酸酶, 甘草, VitC, 黑色素

Abstract: AIM: To study the inhibitory effects of the licoric extracts on the activity of tyrosinase, which is constituted of monophenolase and diphenolase.METHODS: The 50%(V/V) alcohol is used as extractant.To investigate the effects of the licoric extracts on the monophenolase and diphenolase activity, the absorbance of the solution containing several concentrations of the licoric extracts was measured at 475 nm and 390 nm, using the Ltyrosine and pyrocatechin as the substrate, respectively.RESULTS: The licoric extracts has showed a strongly inhibition on the activity of tyrosinase, either monophenolase or diphenolase.The IC50 is 1.94 and 2.94mg·ml-1 , respectively.CONCLUSION: The licoric extracts has inhibitory effects on both monophenolase and diphenolase.The diphenolase activity test system using pyrocatechin as the substrate is a useful procedure to study the activity of tyrosinase.

Key words: tyrosinase, licorice root, VitC, melanin

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