榄香烯抑制表皮生长因子诱导的晶状体上皮细胞增殖的信号转导机制

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (1): 43-46.

榄香烯抑制表皮生长因子诱导的晶状体上皮细胞增殖的信号转导机制

黄秀榕¹, 祁明信², 胡艳红², 严京¹, 吴正正¹, 马兰³

¹浙福建中医学院病理生理研究中心, 福州 350003, 福建;
²福建中医学院附属第二人民医院眼科, 福州 350003, 福建;
³海南医学院病理生理教研室, 海口 571101, 海南

收稿日期:2006-08-02 修回日期:2006-10-08 出版日期:2007-01-26 发布日期:2020-10-26
通讯作者: 黄秀榕, 女, 教授, 博士研究生导师, 主要从事白内障的基础与临床研究。Tel:0591-83570887 　E-mail:qihuang@netease.com
基金资助:
福建省自然科学基金项目资助(C0410030); 福建省医药卫生重点项目资助(W2002504)

Signal transduction mechanism of inhibition of elemene on proliferation induced by recombinant human epidermal growth factor in lens epithelial cell

HUANG Xiu-rong¹, QI Ming-xin², HU Yan-hong², YAN Jing¹, WU Zheng-zheng¹, MA Lan³

¹Research Center of Pathophysiology, Fujian College of Traditional Chinese Medicine, Fuzhou 350003, Fujian, China;
²Department of Ophthalmology, Second Affiliated People' s Hospital of Fujian College of Traditional Chinese Medicine,Fuzhou 350003, Fujian, China;
³Department of Pathophysiology, Hainan Medical College, Haikou 571101, Hainan,China

Received:2006-08-02 Revised:2006-10-08 Online:2007-01-26 Published:2020-10-26

摘要/Abstract

摘要： 目的: 研究榄香烯(elenene, Ele) 抑制重组人表皮生长因子(recombinant human epidermal growth factor, rhEGF) 诱导的晶状体上皮细胞(lens epithelium cell, LEC) 增殖时对细胞内钙(Ca²⁺) 、环磷酸腺苷(cyclic adenosine monophosphate, cAMP) 、环磷酸鸟苷(cyclic guanosine monophosphate, cGMP) 的影响, 探讨其抑制增殖的细胞信号转导机制。方法: 采用rhEGF 诱导LEC 增殖后, 用荧光分光光度法检测LEC 内钙离子浓度([Ca²⁺]_i) 、放射免疫分析法检测LEC 内cAMP 和cGMP 含量, 探讨Ele 的影响。结果: (1) Ele 组LEC 内[Ca²⁺]_i 比增殖组显著增高(P<0.01);(2) 增殖组LEC 内cAMP 浓度比对照组明显下降、cGMP 浓度比对照组明显升高(P<0.01);(3) Ele 组LEC 内cAMP 浓度比增殖组显著升高、cGMP 浓度比增殖组显著降低(P<0.01) 。结论: Ele抑制LEC 增殖的作用是通过细胞内[Ca²⁺]_i 、cAMP和cGMP 信号系统及多条信号转导途径的相互作用实现的, 这可能是Ele 抑制LEC 增殖、防治后发性白内障的细胞和分子生物学机制。

关键词: 榄香烯, 晶状体上皮细胞, 白内障, 表皮生长因子, 增殖, 钙, 环磷酸腺苷, 环磷酸鸟苷

Abstract: AIM: To investigate the effects of elemene(Ele) on intracellular concentrations of calcium([Ca²⁺]_i), cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in lens epithelial cell (LEC) of proliferation induced by recombinant human epidermal growth factor (rhEGF), and the mechanism of signal transduction of inhibiting proliferation. METHODS: Proliferations of LEC were induced by rhEGF;[Ca²⁺]_i concentrations of LEC were determined by spectrofluoremeter;The intracellular concentrations of cAMP and cGMP were measured by radioimmunoassay. RESULTS: (1) [Ca²⁺]_i of LEC in the Ele group increased obviously compared with the proliferation group(P<0.01);(2) Concentrations of cAMP of LEC in the proliferation group decreased compared with the control group.Concentrations of cGMP of LEC in the proliferation group increased compared with the control group (P<0.01);(3) Concentrations of cAMP of LEC in the Ele group increased significantly compared with the proliferation group.Concentrations of cGMP of LEC in the Ele group decreased compared with the proliferation group(P<0.01). CONCLUSION: Elemene inhibits proliferation of LEC by means of signal transduction of [Ca²⁺]_i, cAMP, cGMP and interaction of multiple signal transduction, which may be the cellular and molecular mechanism of Ele inhibiting/LEC proliferation and preventing after cataract.

Key words: elemene, lens epithelial cell, cataract, proliferation, epidermal growth factor, Ca²⁺, cyclic adenosinemonophosphate, cyclic guanosine monophosphate

中图分类号:

R965.2

黄秀榕, 祁明信, 胡艳红, 严京, 吴正正, 马兰. 榄香烯抑制表皮生长因子诱导的晶状体上皮细胞增殖的信号转导机制[J]. 中国临床药理学与治疗学, 2007, 12(1): 43-46.

HUANG Xiu-rong, QI Ming-xin, HU Yan-hong, YAN Jing, WU Zheng-zheng, MA Lan. Signal transduction mechanism of inhibition of elemene on proliferation induced by recombinant human epidermal growth factor in lens epithelial cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(1): 43-46.

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