重组人血小板生成因子对辐射后C57 小鼠及MO7e 细胞的保护作用

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (1): 66-71.

重组人血小板生成因子对辐射后C57 小鼠及MO7e 细胞的保护作用

苏颖杰¹, 李晶², 姚明辉¹, 贡沁燕¹

¹浙复旦大学上海医学院药理学系, 上海 200032;
²上海中信国健药业有限公司, 上海 201203

收稿日期:2006-10-14 修回日期:2006-11-10 出版日期:2007-01-26 发布日期:2020-10-26
通讯作者: 贡沁燕, 女, 教授, 硕士研究生导师, 研究方向:心血管、神经激素药理学。Tel:021-54237228 　E-mail: gongqinyan@yahoo.com.cn
作者简介:苏颖杰, 女, 硕士研究生, 研究方向:心血管药理学。Tel:021-54237690 　E-mail: irissyj@yahoo.com.cn

Radioprotective effect of rhTPO on mice and MO7e cells

SU Ying-jie¹, LI Jing², YAO Ming-hui¹, GONG Qin-yan¹

¹Department of Pharmacology, Shanghai Medical College, Fudan University, Shanghai 200032, China;
²CP Guojian Pharmaceutical Co., Ltd., Shanghai 201203, China

Received:2006-10-14 Revised:2006-11-10 Online:2007-01-26 Published:2020-10-26

摘要/Abstract

摘要： 目的: 观察国产重组人血小板生成因子(rhTPO)对辐射所致C57 小鼠血小板减少的治疗作用及对辐射所致MO7e 细胞凋亡的影响。方法: 雄性C57小鼠137铯γ射线4Gy 全身照射, 于照射前及照射后不同时间点剪尾取血, 测定血小板数。MTT 法测定辐射后MO7e 细胞的存活率, 流式细胞仪、透射电镜观察MO7e 细胞的凋亡。结果: rhTPO 4.5~18 μg/kg 可剂量依赖性地升高由137铯辐射降低的C57 小鼠外周血小板水平, 其升高血小板作用比白介素11 强而快。在体外实验中, rhTPO 10 、100 μg/L能明显提高MO7e 细胞存活率, 减少辐射引起的细胞凋亡。结论: 国产重组人血小板生成因子能降低辐射对小鼠造血系统的损伤, 减少辐射引起的MO7e 细胞的凋亡, 升高细胞存活率, 具有一定的辐射保护作用。

关键词: 重组人血小板生成因子, 血小板减少, 辐射防护, 细胞凋亡

Abstract: AIM: To study the therapeutic action of homemade rhTPO on thrombocytopenia of irradiated C57 mice and the effect of homemade rhTPO on apoptosis of MO7e cells induced by irradiation. METHODS: The male C57mice underwent total body irradiation(TBI) with a single 4 Gy dose using a 137Cs γ-rays source, and the blood samples were taken at different time points pre- and post-irradiation by cutting tails, and the platelet counts were determined.MTT assay was used to measure the rate of irradiated MO7e cells.The apoptosis of MO7e cells was observed with flow cytometer and E.M.microscopy. RESULTS: RhTPO (4.5-18μg/kg) dose-dependently rose the circulating platelet counts of C57 mice reduced by irradiation and its effect was more powerful and faster than that of rhIL-11.In vitro, rhTPO 10 and 100 μg/L obviously increased the viability rate of MO7e cells and reduced the apoptosis rate of MO7e cells upon irradiation. CONCLUSION: The homemade rhTPO has the capability of reducing the damage of hematopoietic system in irradiated mice, decreasing the apoptosis rate of MO7e cells upon irradiation and increasing the viability rate of MO7e cells.rhTPO has significant protective effect on mice and MO7e cells against irradiation injury.

Key words: recombinant human thrombopoietin, thrombocytopenia, radioprotection, apoptosis

中图分类号:

苏颖杰, 李晶, 姚明辉, 贡沁燕. 重组人血小板生成因子对辐射后C57 小鼠及MO7e 细胞的保护作用[J]. 中国临床药理学与治疗学, 2007, 12(1): 66-71.

SU Ying-jie, LI Jing, YAO Ming-hui, GONG Qin-yan. Radioprotective effect of rhTPO on mice and MO7e cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(1): 66-71.

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