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中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (1): 93-97.

• 定量药理学 • 上一篇    下一篇

重组人干扰素α2a在小鼠和大鼠体内i.v.和i.m.的药动学研究

路莉, 崔明霞, 王莉, 张宝来, 吴勇杰   

  1. 兰州大学药学院药理学研究所, 甘肃省新药临床前研究重点实验室, 兰州 730000, 甘肃
  • 收稿日期:2006-10-16 修回日期:2006-10-23 出版日期:2007-01-26 发布日期:2020-10-26
  • 通讯作者: 吴勇杰, 男, 教授, 硕士生导师, 研究方向:生物制品临床前研究。Tel:0931-3661828  E-mail: lywyj@pvblic.lz.gs.cn
  • 作者简介:路莉, 女, 讲师, 研究方向:药物代谢动力学。Tel:13919969446 E-mail: lul@lzu.edu.cn

Pharmacokinetic study of rh-IFNα2a in mice and rats after intravenous and intramuscular administration

LU Li, CUI Ming-xia, WANG Li, ZHANG Bao-lai, WU Yong-jie   

  1. Pharmacology Research Institute of Pharmacy College, Lanzhou University, Pharmacology Lab of Gansu Province Key Laboratory of Preclinircal study for new drugs, Lanzhou 730000, Gansu, China
  • Received:2006-10-16 Revised:2006-10-23 Online:2007-01-26 Published:2020-10-26

摘要: 目的: 研究rh-干扰素α2a(rh-IFNα2a) 在小鼠和大鼠体内i.v.和i.m.的血药浓度-时间曲线、药动学参数和分布、排泄特点。方法: 用双抗体夹心ELISA 法测rh-IFNα2a 在小鼠和大鼠体内i.v.和i.m.后血清、胆汁、尿液及组织中的药物浓度, 用DAS 药动学统计软件进行血药浓度-时间曲线拟合及药动学参数计算, 并结合免疫组织化学技术研究rh-IFNα2a 的组织分布特点。结果: Rh-IFNα2a i.v.和i.m的药动学行为分别符合二室和一室开放模型, 呈一级动力学消除;rh-IFNα2a 主要分布在肾、肺组织中;rh-IFNα2a 36 h 尿累计排泄量为0.121 %, 胆汁累计排泄量为0.247 %。结论: I.v.、i.m.rh-IFNα2a 的药动学行为分别符合二室一级消除、一室一级消除。

关键词: 干扰素, 药动学, 酶联免疫吸附测定

Abstract: AIM: To study pharmacokinetic property of rh-IFNα2a after intravenous (i.v.) and intramuscular (i.m.) injection to mice and rats. METHODS: Double antibody sandwich ELISA analysis was used for testing drug concentration in serum, urine, bile and tissues after i.v.and i.m.administration of rh-IFNα2a in mice and rats.Pharmacokinetic parameters were calculated by DAS software.Immunohistochemistry technique was also adopted to evaluate its distribution characters. RESULTS: Pharmacokinetic model of rh-IFNα2a after i.v.and i.m. was consistent with two-compartment and one-compartment open model respectively, both submitting to first-order kinetic elimination.Rh-IFNα2a was predominantly distributed in kidney and lung tissue.The total drug accumulative excretion quantity in urine was 0.121 %of the administered after rh-IFNα2a 8.19 μg/kg i.v.into mice. The total drug accumulative excretion quantity in bile was 0.247 %of the administered after rh-IFNα2a 4.10 μg/kg i.v.into rats. CONCLUSION: Pharmacokinetic models after i.v.and i.m.rh-IFNα2a are two-compartment open model and one-compartment open model, respectively, both with first-order kinetic elimination.

Key words: interferon, pharmacokinetics, enzymelinked immunosorbent assay

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