中药牛至单体体外抗阴道毛滴虫作用的研究

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (2): 182-186.

中药牛至单体体外抗阴道毛滴虫作用的研究

迟秀玲¹, 杨秋林², 朱炳阳¹, 严宇桢³, 温曙光⁴, 李莲³, 颜涛¹, 张海强¹, 钟超¹, 谭海平³, 严奉祥¹

¹南华大学药物药理研究所, ²病原微生物研究所, ³生命科学院药理系,衡阳 421001, 湖南;
⁴山东农业大学生命科学院食品科学与工程系, 泰安 271018, 山东

收稿日期:2006-07-17 修回日期:2006-11-03 出版日期:2007-02-26 发布日期:2020-10-27
通讯作者: 严奉祥,男, 教授, 研究生导师, 留美学者, 研究方向:分子药理学和药物开发。
基金资助:
湖南省卫生厅基金资助项目(204062); 湖南省教育厅重点基金资助项目(03A040); 国家教育部重点基金资助项目(204095); 国家“十五”攻关项目(02EFN214300457)

Effect of oregano monomers against Trichomonas vaginalis in vitro

CHI Xiu-ling¹, YANG Qiu-lin², ZHU Bing-yang¹, YAN Yu-zhen³, WEN Shu-guang⁴, LI Lian³, YAN Tao¹, ZHANG Hai-qiang¹, ZHONG Chao¹, TAN Hai-ping³, YAN Feng-xiang¹

¹Institute of Drug and Pharmacology, ²Institute of Pathogenic Microorganism, ³Undergraduate Student, University of Nanhua, Hengyang 421001, Hunan, China;
⁴Undergraduate Student at Shandong Agriculture University, Taian 271018, Shandong, China

Received:2006-07-17 Revised:2006-11-03 Online:2007-02-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 检测中药牛至单体百里香酚、香荆芥酚、迷迭香酸和咖啡酸体外抗阴道毛滴虫的作用, 以寻找不良反应少、疗效较高的药物。方法: 观察牛至单体在不同时间和不同浓度对培养的阴道毛滴虫虫体形态的变化、存活率(%)和最低有效浓度。结果: 百里香酚和香荆芥酚在5 mmol/L 杀灭阴道毛滴虫效应比甲硝唑快1 倍;而百里香酚和香荆芥酚最低有效浓度分别为1.25 、2.5 mmol/L, 比甲硝唑分别弱1 和2 倍。迷迭香酸和咖啡酸抗滴虫效应比较弱。结论: 百里香酚和香荆芥酚具有较强杀灭阴道毛滴虫作用。

关键词: 百里香酚, 香荆芥酚, 迷迭香酸, 咖啡酸, 甲硝唑, 阴道毛滴虫, 最低有效浓度

Abstract: AIM: To study the effect of four monomers (thymol, cavacrol, rosmarinic acid and caffeic acid) extracted from oregano against Trichomonas vaginalis. MERHODS: The morphologic change, survival rate and MEC of the monomers on Trichomonas vaginalis were observed in different times and at different concentrations in vitro.RESULTS: The initial effect of Thymol and Cavacrol(5 mmol/L)against Trichomonas vaginalis was one time quicker than metronidazole.The MEC of thymol and cavacrol was 1.25, 2.5 mmol/L respectively at 24 h, which was respectively one and two times weaker than metronidazole.The effect of rosmarinic acid and caffeic acid on Trichomonas vaginalis was weak.CONCLUSIONS: Thymol and cavacrol have stronger effect against Trichomonas vaginalis.

Key words: thymol, cavacrol, rosmarinic acid, caffeic acid, metronidazole, Trichomonas vaginalis, minimal effective concentration

中图分类号:

R965.2

迟秀玲, 杨秋林, 朱炳阳, 严宇桢, 温曙光, 李莲, 颜涛, 张海强, 钟超, 谭海平, 严奉祥. 中药牛至单体体外抗阴道毛滴虫作用的研究[J]. 中国临床药理学与治疗学, 2007, 12(2): 182-186.

CHI Xiu-ling, YANG Qiu-lin, ZHU Bing-yang, YAN Yu-zhen, WEN Shu-guang, LI Lian, YAN Tao, ZHANG Hai-qiang, ZHONG Chao, TAN Hai-ping, YAN Feng-xiang. Effect of oregano monomers against Trichomonas vaginalis in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(2): 182-186.

参考文献

[1] Cudmore SL, Delgaty KL, Hayward-McClell SF, et al. Treatment of infections caused by Metronidazole-resistant Trichomonas vaginalis[J]. Clin Microbiol Rev, 2004;17:783-793.
[2] Sisson G, Goodwin A, Raudonikiene A, et al. Enzymes associated with reductive activation and action of Nitazoxanide, Nitrofurans, and Metronidazole in Helicobacter pylori[J]. Antimicrob Agents Chemother, 2002;46:2116-2123.
[3] 马红钗, 沈国理, 戚佳叶, 等.甲硝唑的不良反应[J]. 现代中西医结合, 2003;12:672-673.
[4] 孙宏伟, 陈殿学, 李晓燕.复方蛇床子使君子对阴道毛滴虫体外作用的研究[J]. 中医药学刊, 2002;20:367.
[5] 张登霞. 中药花椒体外抗阴道毛滴虫的作用研究[J]. 兰州医学院学报, 2003;29:21-23.
[6] 吴杰, 张敏如, 丁东宁, 等.雀石蕊体外杀阴道毛滴虫作用[J]. 中国寄生虫学与寄生虫病杂志, 1995;13:126-129.
[7] 闫艳, 高兴政.白头翁体外抗阴道毛滴虫的效果及扫描电镜观察[J]. 中国寄生虫病防治杂志, 2003;16:353-355.
[8] Kobayashi S, Watanabe J, Fukushi E, et al. Polyphenols from some foodstuffs as inhibitors of ovalbumin permeation through Caco-2 cell monolayers[J]. Biosci Biotechnol Biochem, 2003;67:1250-1257.
[9] Exarchou V, Godejohann M, van Beek TA, et al. LC-UV-solidphase extraction-NMR-MS combined with a cryogenic flow probe and its application to the identification of compounds present in Greek oregano[J]. Anal Chem, 2003;75:6288-6294.
[10] 常正山. 抗滴虫药物实验方法[A]. 见:徐叔云, 卞如濂,陈修, 主编.药理实验方法学[M]. 第3 版.北京:人民卫生出版社, 2003:1613-1616.
[11] 刘斌. 细胞培养中的研究方法[A]. 见:司徒镇强, 吴军正, 主编.细胞培养[M]. 第1 版.西安:世界图书出版公司, 2004:246-248.
[12] Jerkovic I,Mastelic J, Milos M.The effect of air-drying on glycosidically bound volatiles from seasonally collected oregano (Origanum vulgare ssp.hirtum) from Croatia[J]. Nahrung,2001;45:47-49.
[13] Guenthner PC, Secor WE, Dezzutti CS.Trichomonas vaginalisinduced epithelial monolayer disruption and human immunodeficiency virus type 1 (HIV-1) replication:implications for the sexual transmission of HIV-1[J]. Infect Immun, 2005;73:4155-4160.
[14] Sun B,Wang H, LuM.Volatile Comparison of Chinese Medicine Herb ThymusMongolicus[J]. Acta Bot Boreaccident Sin, 2001;21:990-996.
[15] Matthys H, de Mey C, Carls C, et al. Efficacy and tolerability of myrtol standardized in acute bronchitis:a multi-centre, randomised, double-blind, placebo-controlled parallel group clinical trial vs cefuroxime andambroxol[J]. Arzneimittel Forsch, 2000;50:700-711.
[16] Chami F, Chami N, Bennis S, et al. Evaluation of carvacrol and eugenol as prophylaxis and treatment of vaginal candidiasis in an immunosuppressed rat model[J]. J Antimicrob Chemother,2004;54:909-914.
[17] Petersen M, Simmonds MSJ.Molecules of Interest Rosmarinic acid[J]. Phytochemistry, 2003;62:121-125.
[18] Chung TW, Moon SK, Chang YC, et al. Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepatocarcinoma cells:complete regression of hepatoma growth and metastasis by dual mechanism[J]. Faseb J, 2004;18:1670-1681.