胰岛素样生长因子-1 对小鼠胰岛细胞凋亡的保护作用

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (6): 645-648.

胰岛素样生长因子-1 对小鼠胰岛细胞凋亡的保护作用

刘晓红¹, 陈洲², 王燕萍¹, 刘礼斌¹

¹福建医科大学附属协和医院内分泌科, 福州350001, 福建;
²福建医科大学药学院药理教研室, 福州350004, 福建

收稿日期:2007-05-17 修回日期:2007-06-04 发布日期:2020-11-09
通讯作者: 陈洲, 女, 硕士, 研究方向:胰岛β 细胞保护。Tel:0591-83569029 E-mail:Kellandchen1007 @Yahoo. com.cn
作者简介:刘晓红, 女, 本科, 研究方向:胰岛β 细胞保护。Tel:0591-83357896-8452 E-mail:dokidoki@163. com
基金资助:
福建省教育厅资助项目(JA03087)

Protective effect of insulin-like growth factor-1 on apoptosis in murine pancreatic β-cells

LIU Xiao-hong¹, CHEN Zhou², WANG Yan-ping¹, LIU Li-bin¹

¹Department of Endocrinology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian, China;
²Department of Pharmacology, College of Pharmaceutical Sciences, Fujian Medical University, Fuzhou 350004, Fujian, China

Received:2007-05-17 Revised:2007-06-04 Published:2020-11-09

摘要/Abstract

摘要： 目的:探讨胰岛素样生长因子-1(IGF-1)对第三丁基过氧化氢(tBHP)诱导小鼠胰岛β 细胞株MIN6 凋亡的保护作用。方法:体外培养小鼠胰岛β细胞株MIN6, 分为:对照组、tBHP(25 μmol/L)组、IGF-1(100 ng/mL)组和IGF-1 +tBHP 组。丫啶橙-溴化乙锭(AO-EB)荧光染色法及原位末端标记法(TUNEL)镜下观测各实验组细胞凋亡情况,Griess 法检测各实验组细胞内NO 水平。结果:25 μmol/L tBHP作用1 h 可诱导MIN6 细胞凋亡, 并使细胞内NO含量明显增加。预先经过100 ng/mL IGF-1 作用24 h 后, tBHP 诱导的MIN6 细胞凋亡明显减少, 同时细胞内NO 含量亦显著降低。结论:IGF-1 对tBHP诱导凋亡的小鼠胰岛β 细胞株MIN6 有一定的保护作用, 其机制可能与IGF-1 减少tBHP 诱导的NO 合成有关。

关键词: 胰岛素样生长因子-1, 胰岛β 细胞, 第三丁基过氧化氢, 凋亡, 保护

Abstract: AIM:To investigate the protective effect of insulin-like growth factor-1 (IGF-1)on apoptosis in murine pancreatic β-cellsMIN6 induced by tert-butyl hydroperoxide(tBHP).METHODS: MIN6 cells were cultured and divided into four groups: control group, tBHP (25 μmol/L)group, IGF-1(100 ng/mL)group and IGF-1 +tBHP group. Cell damage was detected by AO-EB staining and TUNEL assay. The levels of nitric oxidewere measured by Griess assay.RESULTS:Induced cell apoptosis and increased NO production were observed when MIN6 cells treated with 25 μmol/L of tBHP for 1 h, When the cells were pretreated with 100 ng/mL IGF-1 for 24 h before tBHP exposured, cell apoptosis induced by tBHP was significantly inhibited and NO production was also decreased.CONCLUSION:The present study suggests that IGF-1 has protective effect on apoptosis in murine pancreatic β-cells MIN6 induced by tBHP. The mechanism of this effect is possibly attributed to IGF-1 reduces NO production which induced by tBHP.

Key words: insulin-like growth factor-1, pancreatic β-cell, tert-butyl hydroperoxide, apoptosis, protection

中图分类号:

R965. 2

刘晓红, 陈洲, 王燕萍, 刘礼斌. 胰岛素样生长因子-1 对小鼠胰岛细胞凋亡的保护作用[J]. 中国临床药理学与治疗学, 2007, 12(6): 645-648.

LIU Xiao-hong, CHEN Zhou, WANG Yan-ping, LIU Li-bin. Protective effect of insulin-like growth factor-1 on apoptosis in murine pancreatic β-cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(6): 645-648.

参考文献

1 Evans JL, Goldfine ID, Maddux BA, et al. Oxidative stress and stress-activated signaling pathways:a unifying hypothesis of type 2 diabetes[J]. Endocr Rev, 2002; 23:599-622.
2 Robertson RP, Harmon J, Tran PO, et al. Glucose toxicity in beta-cells: type 2 diabetes, good radicals gone bad, and the glutathione connection[J]. Diabetes, 2003; 52:581-587.
3 Sakuraba H, Mizukami H, Yagihashi N, et al. Reduced betacell mass and expression of oxidative stress-related DNA damage in the islet of Japanese Type Ⅱ diabetic patients[J]. Diabetologia, 2002;45:85-96.
4 Ceriello A, Piconi L, Quaqliaro L, et al. Effects of pramlintide on postprandial glucose excursions and measures of oxidative stress in patients with type 1 diabetes[J]. Diabetes Care, 2005; 28: 632-637.
5 Ueki K, Okada T, Hu J, et al. Total insulin and IGF-I resistance in pancreatic beta cells causes overt diabetes [J]. Nat Genet, 2006; 38:583-588.
6 Mabley JG, Belin V, John N, et al. Insulin-like growth factor Ⅰ reverses interleukin-1β inhibition of insulin secretion, induction of nitric oxide synthase and cytokine-mediated apoptosis in rat islets of Langerhans[J]. FEBS Lett, 1997;417: 235-238.
7 TanaK_a Y, Tran PO, Harmon J, et al. A role for glutathione peroxidase in protecting pancreatic beta cells against oxidative stress in a model of glucose toxicity[J]. Proc Natl Acad Sci US A, 2002; 99:12363-12368.
8 王燕萍.氧化应激可通过NF-κB-iNOS-NO 信号通路诱导小鼠胰岛β 细胞凋亡[J]. 中国病理生理杂志, 待发表.
9 朱元贵, 陈晓春, 陈志哲, 等.第三丁基过氧化氢损伤皮层神经元线粒体并诱导凋亡[J]. 中国病理生理杂志, 2005; 21:2014-2017.
10 周永列, 吕亚萍, 邱莲女, 等.硝普钠抑制K562 细胞增殖并诱导细胞凋亡的实验研究[J]. 中国临床病理学与治疗学杂志, 2004; 9:1381-1387.
11 Kaneto H, Fujii J, Seo HG, et al. Apoptotic cell death triggered by nitric oxide in pancreatic beta-cells[J]. Diabetes, 1995;44: 733-738.
12 李强, 许霖水.外源一氧化氮对大鼠胰岛细胞损伤机制研究[J]. 西北国防杂志, 2002; 23:361-363.
13 Wrede CE, Dickson LM, Linqohr MK, et al. Protein kinase B Akt prevents fatty acid-induced apoptosis in pancreatic betacells (INS-1)[J]. J Biol Chem, 2002; 277:49676-49684.
14 Hill DJ, Petrik J, Aranv E, et al. Insulin-like growth factors prevent cytokine-mediated cell death in isolated islets of Langerhans from prediabetic non-obese diabetic mice[J]. J Endocrinol, 1999;161:153-165.

[1]	王金刚, 李强, 孙会艳, 王洪权. 鼠尾草酸在帕金森病中的神经保护作用机制研究进展[J]. 中国临床药理学与治疗学, 2023, 28(9): 1073-1080.
[2]	王坤, 许佩佩, 周兰兰, 鲁晟. 人参皂苷Rg1调控Epac1/Rap1信号通路对缺血性脑卒中大鼠神经保护作用机制研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 721-727.
[3]	范杰, 金永明, 江孝龙, 蒋国华. lncRNA HOTAIR调控miR-206影响类风湿关节炎滑膜细胞增殖和凋亡的分子机制研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 736-742.
[4]	杨晓佳, 江萌, 吴敏, 张伊黎, 吕兰, 吴嫄芬, 王鑫昱, 刘立权. 藏红花素介导DKK3调控GSK-3β/β-catenin通路对阿尔兹海默症大鼠的认知改善作用[J]. 中国临床药理学与治疗学, 2023, 28(5): 489-497.
[5]	侯小煜, 冷玉芳, 曹雪芬, 吕兴娇, 韩晓霞, Janvier NIBARUTA, 刘永强. 五味子乙素减轻小鼠肠缺血再灌注损伤的网络药理学分析及实验验证[J]. 中国临床药理学与治疗学, 2023, 28(2): 147-154.
[6]	王剑, 孙永东, 周兴玮, 刘雷, 陈龙, 童兴科, 朱佳丽. 黄芩素经由miR-125b-5p/IRF4轴进而促进喉癌细胞死亡并抑制其侵袭的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1209-1218.
[7]	朱瑞芳, 郭东凯, 智慧, 江翊国, 张悦翎, 钱晓萍, 季士亮. ROS-NF-κB-p38MAPK通路探索榄香烯联合硼替佐米抗多发性骨髓瘤的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1219-1226.
[8]	周欢, 徐铭, 李波, 刘春英, 王淳. PI3K/Akt/FOXO3a信号通路介导的保护性自噬参与肺腺癌获得性顺铂耐药表型重塑[J]. 中国临床药理学与治疗学, 2022, 27(9): 961-970.
[9]	华海燕, 严杰, 秦宇芬. 五味子乙素通过抑制心肌细胞凋亡减轻大鼠心肌缺血再灌注损伤的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(8): 848-856.
[10]	李雪恒, 李龙. 褪黑素与特发性肺纤维化关系的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(6): 715-720.
[11]	吕兴娇, 冷玉芳, 韩晓霞, 侯小煜, 曹雪芬, Janvier NIBARUTA, 刘永强. 短链脂肪酸减轻脏器缺血-再灌注损伤的研究进展#br#[J]. 中国临床药理学与治疗学, 2022, 27(2): 219-226.
[12]	杨柳, 罗爱林, 李世勇. 全麻药物损伤发育期大脑的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(12): 1381-1390.
[13]	谭峰浪, 任宏丽, 王宏飞. 黄柏提取物对Aβ_1-42诱导的海马神经细胞的影响[J]. 中国临床药理学与治疗学, 2022, 27(11): 1213-1220.
[14]	潘蓉蓉, 支英豪, 金永喜, 周夏慧. 茶黄素调节CaMKK2/AMPK信号通路对脑出血大鼠神经元凋亡和血脑屏障的影响[J]. 中国临床药理学与治疗学, 2022, 27(11): 1240-1246.
[15]	李莹, 姚伟, 王萌, 余志宏, 龚园其, 蓝海兵, 齐协飞. 鸢尾素通过NF-κB和JNK信号通路减轻屋尘螨诱导的气道上皮细胞炎症及凋亡的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(10): 1106-1112.