提高人肺腺癌裸鼠皮下移植瘤成瘤率的方法学研究

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (9): 1052-1057.

提高人肺腺癌裸鼠皮下移植瘤成瘤率的方法学研究

张宝来, 徐明丽, 高明堂, 路莉, 王莉, 崔明霞, 李文广, 吴勇杰

兰州大学药学院药理学研究所, 甘肃省新药临床前研究重点实验室, 兰州730000, 甘肃

收稿日期:2007-07-05 修回日期:2007-09-08 出版日期:2007-09-26 发布日期:2020-10-30
通讯作者: 吴勇杰, 男, 教授, 硕士生导师, 研究方向:新药药理学。Tel:0931-3661828 E-mail:lywyj@r5you.com
作者简介:张宝来, 男, 助教, 研究方向:肿瘤药理学。Tel:13659482179 E-mail:john chinese@sohu.com

Methodological study on improving tumor formation rate of xenografted human lung cancer in nude mice

ZHANG Bao-lai, XU Ming-li, GAO Ming-tang, LU Li, WANG Li, CUI Ming-xia, LI Wen-guang, WU Yong-jie

Pharmacology Research Institute of Pharmacy College, Pharmacology Lab of Gansu ProvinceKey Laboratory of Preclinical Study for New Drugs, Lanzhou 730000, Gansu, China

Received:2007-07-05 Revised:2007-09-08 Online:2007-09-26 Published:2020-10-30

摘要/Abstract

摘要： 目的:提高人肺腺癌裸鼠皮下移植瘤的成瘤率。方法:人肺腺癌SPC-A-1、GLC-82 培养细胞或相应的瘤组织匀浆接种于裸鼠皮下之前, 先用富含生长因子的小鼠肉瘤S180 腹水上清液按比例稀释, 然后在裸鼠背部或腋窝皮下接种。观察人肿瘤细胞或肿瘤组织在加入小鼠肉瘤S180 腹水上清液后, 对移植瘤生长速度及成瘤率的影响。结果:加入小鼠肉瘤S180 腹水上清液后, 人肺腺癌SPC-A-1 和GLC-82成瘤率明显提高, 而且移植后的人肿瘤生长速度较未加小鼠肉瘤S180 腹水上清液的人肺腺癌细胞明显加快。结论:小鼠肉瘤S180 腹水上清液能明显提高人肺腺癌SPC-A-1 和GLC-82 裸鼠皮下移植瘤的成瘤率, 促进肿瘤生长。

关键词: 人肺癌, 裸小鼠, 肿瘤模型, 抑制率

Abstract: AIM: To improve tumor formation rate of xenografted human lung cancer in nude mice.METHODS: Before xenograftion, human lung cancer SPC-A-1 or GLC-82 cells and their tissue homogenate were diluted with supernatant of mice sarcoma 180 ascites being rich in growth factors.The diluted SPC-A-1 or GLC-82 cell suspensions were injected subcutaneously at right back or armpit of nude mice.The effects of supernatant of mice sarcoma 180 ascites on the tumor growth rate and tumor formation rate were evaluated.RESULTS: Compared with control group, the tumor growth rate and tumor formation rate of human lung cancer SPC-A-1 or GLC-82 in nude mice were significantly improved with the addition of the supernatant of mice sarcoma 180 ascites.CONCLUSION: The supernatant of mice sarcoma 180 ascites can significantly improve the tumor growth rate and tumor formation rate of human lung cancer SPC-A-1 or GLC-82 in nude mice.

Key words: human lung cancer, nude mice, tumor model, inhibition rate

中图分类号:

R965.2

张宝来, 徐明丽, 高明堂, 路莉, 王莉, 崔明霞, 李文广, 吴勇杰. 提高人肺腺癌裸鼠皮下移植瘤成瘤率的方法学研究[J]. 中国临床药理学与治疗学, 2007, 12(9): 1052-1057.

ZHANG Bao-lai, XU Ming-li, GAO Ming-tang, LU Li, WANG Li, CUI Ming-xia, LI Wen-guang, WU Yong-jie. Methodological study on improving tumor formation rate of xenografted human lung cancer in nude mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(9): 1052-1057.

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