丹参酮ⅡA对自发性高血压大鼠左心室谷胱甘肽-s-转移酶μ2表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (1): 57-61.

丹参酮ⅡA对自发性高血压大鼠左心室谷胱甘肽-s-转移酶μ2表达的影响

周四桂^1,2, 王平¹, 王朝禾¹, 潘雪刁², 刘培庆¹

¹中山大学药学院药理与毒理学实验室, ²广东药学院药理教研室, 广州 510006, 广东

收稿日期:2007-12-06 修回日期:2007-12-27 出版日期:2008-01-26 发布日期:2020-10-13
通讯作者: 刘培庆, 男, 博士, 教授, 博士研究生导师, 研究方向:心血管药理学。Tel:020-87334613 E-mail:sps02@gzsums.edu.cn
作者简介:周四桂, 女, 博士, 讲师, 研究方向:心血管药理学。Tel:020-33359437 　E-mail:susanzsg@163.com
基金资助:
国家自然科学基金资助项目(30672459); 广东省医学科研课题资助项目(A2007309); 广东药学院博士启动科研课题资助项目(43540140)

Effects of tanshinone ⅡA on the expression of glutathione-s-transferaseμ2 in spontaneously hypertensive rats

ZHOU Si-gui^1,2, WANE Ping¹, WANG Zhao-he¹, PAN Xue-diao², LIU Pei-qing¹

¹Lab of Pharmacology &Toxicology, School of Pharmaceutical Sciense, Sun Yat-sen University, ²Pharmacology Department of Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China

Received:2007-12-06 Revised:2007-12-27 Online:2008-01-26 Published:2020-10-13

摘要/Abstract

摘要： 目的观察丹参酮ⅡA 对自发性高血压大鼠(SHR) 左心室谷胱甘肽-s-转移酶μ2(glutathiones-transferase μ2, GSTμ2) 表达的影响, 探讨丹参酮ⅡA 防治左心室肥厚的机制。方法实验分3 组:(1) 正常对照组;(2) SHR 组;(3) 丹参酮ⅡA 组。处理后检测血流动力学指标、左室重量指数及大鼠左心室GSTμ2 的活性, 采用共聚焦显微镜检测左心室O2^- 的产生情况, Western blotting 检测GSTμ2 的表达情况。结果 SHR 组血压与左室重量指数均显著高于正常对照组, 左心功能明显下降;丹参酮ⅡA 显著逆转了SHR 的左心室肥厚,但未明显改善高血压。SHR 组左心室O2^-的产生较正常对照组显著增多, 而丹参酮ⅡA 治疗后,O2^-产生较SHR 组显著减少。GSTμ2 的表达及活性在SHR 组显著降低, 而丹参酮ⅡA 显著增加了GSTM2 的表达及活性。结论丹参酮ⅡA 能逆转SHR 的左心室肥厚, 这可能与其增加GSTμ2 表达, 减少活性氧产生, 消除氧化应激有关。

关键词: 丹参酮ⅡA, 左心室肥厚, 自发性高血压大鼠, 谷胱甘肽-s-转移酶μ2, 氧化应激

Abstract: AIM: To observe the effects of tanshinoneⅡA on the expression of glutathione-s-transferaseμ2 (GSTμ2) in spontaneously hypertensive rats, and to investigate the mechanism of the reversal of left ventricular hypertrophy by tanshinone ⅡA. METHODS: There are 3 groups in the experiment: control, spontaneously hypertensive rats(SHR), SHR treated by tanshinoneⅡA. At the endpoint of the experiment, hemodynamic parameters were measured. Then the rats were sacrificed, body weight (BW) and left ventricular weight (LVW) were measured, and the LVW BW ratios were calculated. The oxidative fluorescence dye dihydroethidium was determined by laser confocal fluorescent microscopy to evaluate in situ O2^-generation in the left ventricles. The expression of GSTμ2 was investigated by western blotting. RESULTS: Compared with control, the systolic blood pressure and LVW to BW ratios of SHR elevated markedly, the left ventricular function was decreased. The left ventricular hypertrophy was significantly reversed by tanshinone ⅡA treatment, but tanshinone ⅡA didn't decrease the systolic blood pressure of SHR. O2^-generation increased in SHR significantly, and it was decreased by tanshinone Ⅱ A. The expression and activities of GSTμ2 in left ventricular significantly decreased in SHR, however that was reversed by tanshinone ⅡA. CONCLUSION: Tanshinone ⅡA can significantly reverse the left ventricular hypertrophy in SHR, that might be associated with up-regulation of GSTμ2, decrease of O2^-and elimination of oxidative stress.

Key words: tanshinone ⅡA, left ventricular hypertrophy, spontaneously hypertensive rats, glutathione-s-transferase μ2, oxidative stress

中图分类号:

R965.2

周四桂, 王平, 王朝禾, 潘雪刁, 刘培庆. 丹参酮ⅡA对自发性高血压大鼠左心室谷胱甘肽-s-转移酶μ2表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(1): 57-61.

ZHOU Si-gui, WANE Ping, WANG Zhao-he, PAN Xue-diao, LIU Pei-qing. Effects of tanshinone ⅡA on the expression of glutathione-s-transferaseμ2 in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(1): 57-61.

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