东莨菪碱对吗啡诱发条件位置性偏爱重现大鼠杏仁核p-CREB及c-Fos表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (1): 89-93.

东莨菪碱对吗啡诱发条件位置性偏爱重现大鼠杏仁核p-CREB及c-Fos表达的影响

赵永娜, 李晓红, 方正梅, 李顺英, 邵晓霞

昆明医学院药学院, 昆明650031, 云南

收稿日期:2007-08-29 修回日期:2007-12-25 出版日期:2008-01-26 发布日期:2020-10-13
作者简介:赵永娜, 女, 硕士, 副教授, 主要从事药物依赖研究。Tel:0871-5338904 　E-mail: mszhaoyn@yahoo.com.cn
基金资助:
云南省教育厅科学技术基金项目(03Y480C)

Effects of scopolamine on expression of p-CREB and c-Fos in amygdala nucleus in rats with recurrence of conditioned place preference induced by morphine

ZHAO Yong-na, LI Xiao-hong, FANG Zheng-mei, LI Shun-ying, SHAO Xiao-xia

Faculty of Pharmaceutical Sciences, Kunming Medical College, Kunming 650031, Yunnan, China

Received:2007-08-29 Revised:2007-12-25 Online:2008-01-26 Published:2020-10-13

摘要/Abstract

摘要： 目的探讨东莨菪碱对吗啡诱发条件位置性偏爱(conditioned place preference, CPP) 重现大鼠杏仁核(amygdala nucleus, Amy) cAMP 反应元件结合蛋白(phospho-cAMP response element bindingprotein, p-CREB) 和c-Fos 表达的变化。方法以剂量递增法建立大鼠CPP 模型, 生理盐水替代吗啡训练大鼠, 使形成的CPP 逐渐消退, 小剂量吗啡激活已消退的CPP。采用免疫组化技术测定不同剂量东莨菪碱对吗啡诱发CPP 重现时大鼠杏仁核p-CREB 和c-Fos 的变化。结果东莨菪碱可抑制吗啡点燃诱发大鼠CPP 重现行为;并可减少吗啡诱发的CPP 重现时大鼠杏仁核p-CREB 和c-Fos 的表达。结论东莨菪碱对小剂量吗啡诱发吗啡依赖大鼠CPP 重现行为的抑制作用可能与其抑制大鼠杏仁核p-CREB 和c-Fos 蛋白表达有关。

关键词: 东莨菪碱, 条件位置性偏爱, cAMP反应元件结合蛋白, c-Fos, 杏仁核

Abstract: AIM: To dectect the effects of scopolamine on expression of phospho-cAMP response element binding protein (p-CREB) and c-Fos in amygdala nucleus (Amy) in rats with recurrence of conditioned place preference(CPP) induced by morphine. METHODS: Morphine was administered by subcutaneous injection at gradually increasing dose(from 10 mg/kg to 60 mg/kg) for 6 days to establish CPP model. From d 7, the rats were administered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection of morphine (4 mg/kg) to reinstate the CPP model, some rats were treated by i. p. scopolamine (1, 2, 3 mg/kg) prior to priminging injection of morphine. The expression of p-CREB and c-Fos in Amy were assayed by immunohistochemistry in the phase of reoccurrence of CPP. RESULTS: After priming injection of morphine 4 mg/kg, the time spent on the drug paired side was significantly reduced because of the treatment with scopolamine, compared with that in the morphine group(P<0.05). Compared with the morphine group, scopolamine decreased the expression of p-CREB and c-Fos of Amy in rats (P<0.05). CONCLUSION: The effects of scopolamine inhibiting morphine CPP reccurrence were probably related to its inhibitory effect on p-CREB and c-Fos expression of Amy in rat.

Key words: morphine, conditioned place preference, cAMP response element binding protein, c-Fos, amygdala nucleus

中图分类号:

R965.2

赵永娜, 李晓红, 方正梅, 李顺英, 邵晓霞. 东莨菪碱对吗啡诱发条件位置性偏爱重现大鼠杏仁核p-CREB及c-Fos表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(1): 89-93.

ZHAO Yong-na, LI Xiao-hong, FANG Zheng-mei, LI Shun-ying, SHAO Xiao-xia. Effects of scopolamine on expression of p-CREB and c-Fos in amygdala nucleus in rats with recurrence of conditioned place preference induced by morphine[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(1): 89-93.

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