胍丁胺对实验性变态反应性脑脊髓炎中枢神经系统内细胞因子IFN-γ、IL-10的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (11): 1226-1230.

胍丁胺对实验性变态反应性脑脊髓炎中枢神经系统内细胞因子IFN-γ、IL-10的影响

潘大津¹, 郑荣远²

¹浙江省丽水市第二医院老年病科, 丽水 323000, 浙江;
²温州医学院附属第一医院神经内科, 温州 325000, 浙江

收稿日期:2008-09-01 修回日期:2008-10-17 出版日期:2008-11-26 发布日期:2020-10-14
通讯作者: 郑荣远, 男, 博士生导师, 研究方向:神经免疫。E-mail: zhengry@yahoo.com.cn
作者简介:潘大津, 男, 医学硕士, 副主任医师, 研究方向:脑血管病。E-mail: hzpdj@163.com
基金资助:
浙江省自然科学基金资助项目(Y204133)

Effects of agmatine on the levels of IFN-γand IL-10 of the central nervous system in experimental allergic encephalomyelitis

PAN Da-jin¹, ZHENG Rong-yuan²

¹Second People’s Hospital of Lishui City, Lishui 323000, Zhejiang, China;
²First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2008-09-01 Revised:2008-10-17 Online:2008-11-26 Published:2020-10-14

摘要/Abstract

摘要： 目的: 观察胍丁胺对实验性变态反应性脑脊髓炎(EAE) 中枢神经系统(CNS) 内细胞因子IFN-γ和IL-10 的影响。方法: 用豚鼠全脊髓匀浆免疫Wistar 大鼠建立EAE 动物模型, 应用双抗体夹心ELISA 法观察不同剂量胍丁胺(分别为25 、50 、100 mg /kg) 对EAE 大鼠脑和脊髓组织内细胞因子IFN-γ和IL-10 水平的影响。结果: 胍丁胺可降低Wistar 大鼠EAE 的发病率, 减轻其临床症状。与空白对照组比较, 模型组大鼠脑和脊髓组织中的IFN-γ水平增加和IL-10 水平降低(P <0.05 或P <0.01), 而胍丁胺干预的3 组大鼠CNS内脑和脊髓组织中的IFN-γ水平降低和IL-10 水平增加。结论: 胍丁胺能调节EAE 中的Th1 /Th2比例平衡, 降低IFN-γ水平, 升高IL-10 水平。

关键词: 实验性变态反应性脑脊髓炎, 胍丁胺, γ-干扰素, 白介素-10

Abstract: AIM: To observe the effects of agmatine(AGM) on the levels of IFN-γand IL-10 of the central nervous system(CNS) in experimental allergic encephalomyelitis(EAE). METHODS: EAE was induced in Wistar rats by immunization of spinal cords homogenate of guinea pigs (GPSCH) and completed Freund’s adjuvant(CFA). EAE rats received AGM intraperitoneally at 25 mg /kg, 50 mg /kg, 100 mg /kg per day for two weeks, respectively, the levels of IFN-γ and IL-10 of the CNS in EAE were observed with the method of sandwich-enzyme-linked inmunosorbent assay (ELISA). RESULTS: AGM could decrease the incidence of EAE, alleviate its clinical manifestation. The level of IFN-γin CNS of model group was significantly increased and the level of IL-10 was significantly decresed, compared with control group (P <0.05 or P < 0.01). In the CNS of three groups treated with different doses of AGM, the level of IL-10 was significantly incresed and the level of IFN-γwas significantly decresed, compared with model group(P<0.05). CONCLUSION: AGM could regulate the ratio of Th1 /Th2 in EAE, increase the level of IL-10 and decrease the level of IFN-γin the CNS of EAE.

Key words: experimental allergic encephalomyelitis, agmatine, IFN-γ, IL-10

中图分类号:

R965.2

潘大津, 郑荣远. 胍丁胺对实验性变态反应性脑脊髓炎中枢神经系统内细胞因子IFN-γ、IL-10的影响[J]. 中国临床药理学与治疗学, 2008, 13(11): 1226-1230.

PAN Da-jin, ZHENG Rong-yuan. Effects of agmatine on the levels of IFN-γand IL-10 of the central nervous system in experimental allergic encephalomyelitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(11): 1226-1230.

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