多肽类药物体内分析方法

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (11): 1309-1314.

多肽类药物体内分析方法

王静, 关勇彪

军事医学科学院毒物药物研究所, 国家北京药物安全评价研究中心, 北京 100850

收稿日期:2008-05-05 修回日期:2008-08-01 出版日期:2008-11-26 发布日期:2020-10-14
通讯作者: 关勇彪,男, 副研究员, 硕士生导师, 研究方向:药代毒代动力学。Tel:010-66931630 E-mail: guanyb@ywap.com.cn
作者简介:王静, 女, 硕士研究生, 研究方向:药代毒代动力学。E-mail: xiujidezhengzi@163.com

In vivo analytical methods for polypeptide drugs

WANG Jing, GUAN Yong-biao

National Beijing Center for Drug Safety Evaluation and Researeh, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China

Received:2008-05-05 Revised:2008-08-01 Online:2008-11-26 Published:2020-10-14

摘要/Abstract

摘要： 多肽类药物是新药研究的新领域, 也是当今新药研究最活跃和发展最迅速的领域之一。目前, 建立测定生物基质中多肽类药物的方法学是世界范围存在的难题, 面临着诸多挑战。酶联免疫法(ELISA) 及放射同位素标记是定量多肽类药物最常用的方法, 应用广泛。但液相色谱-质谱联用技术发展迅速, 高专属性、高灵敏度、应用广泛、简便快速等特点势必使其成为多肽类药物体内定量分析的重要手段。本文就目前国际上几种检测方法的优劣、应用现状及发展趋势进行了综述。

关键词: 多肽药物, 生物检定法, 酶联免疫法, 稳定同位素示踪技术, 放射性同位素示踪技术, 液相色谱-质谱联用技术

Abstract: Polypeptide drugs provide a new field for new drug research, as well as one of the most active and rapid fields of new drug development. At present, how to determine the polypeptide drugs in the biological matrix is a difficulty in the world which faces to a lot of challenges.The widely used methods are ELISA and radiation isotope labeling. HPLC-MS /MS develops fast and will become the most important method for polypeptide drugs determination in vivo. This article will review the pros and cons, application status and development trend of several analytical method used in the world.

Key words: polypeptide drugs, bioassay, ELISA, stable isotope labeling, radiation isotope labeling, LCMS/MS

中图分类号:

R969

王静, 关勇彪. 多肽类药物体内分析方法[J]. 中国临床药理学与治疗学, 2008, 13(11): 1309-1314.

WANG Jing, GUAN Yong-biao. In vivo analytical methods for polypeptide drugs[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(11): 1309-1314.

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