盐酸帕罗西汀对卒中后抑郁大鼠行为和下丘脑室旁核fos 蛋白表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (12): 1360-1364.

盐酸帕罗西汀对卒中后抑郁大鼠行为和下丘脑室旁核fos 蛋白表达的影响

倪贵华¹, 邵蓓², 范红², 黄欢捷²

¹淮安市第一人民医院神经内科, 淮安 223300, 江苏;
²温州医学院附属第一医院神经内科, 温州 325000, 浙江

收稿日期:2008-10-06 修回日期:2008-11-25 发布日期:2020-10-30
作者简介:倪贵华,男,硕士,主治医师,研究方向:主要从事脑血管病的研究。Tel:15996167628 E-mail:Niguihua@yahoo.com.cn.
基金资助:
温州市科技局对外合作重大项目(H2005B031)

Effects of paroxetine on the spontaneous behavior and the c-fos expression of hypothalamus paraventricular nucleus in poststroke depression rats

NI Gui-hua¹, SHAO Bei², FAN Hong², HUANG Huan-jie²

¹Medical Department of Neurology, NO.1 People's Hospital of Huaian, Huaian 223300, Jiangsu, China;
²Medical Department of Neurology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2008-10-06 Revised:2008-11-25 Published:2020-10-30

摘要/Abstract

摘要： 目的探讨抗抑郁药治疗卒中后抑郁的分子作用机制。方法应用线栓法致大脑中动脉闭塞制备脑卒中模型结合束缚应激和孤养法复制了卒中后抑郁症(poststroke depression, PSD) 大鼠模型。观察PSD 大鼠模型行为学改变和下丘脑室旁核c-fos 表达, 及盐酸帕罗西汀对PSD 的干预作用。结果 PSD 模型大鼠蔗糖水消耗量降低、Open-Field 测定直立评分和水平评分减少, 与对照组比较差异有统计学意义(P <0.01);药物组糖水消耗量增加、Open-Field 测定水平得分和直立得分增加, 与PSD 组比较差异有统计学意义(P <0.01)。PSD 组下丘脑室旁核c-fos 蛋白的表达较卒中组或抑郁组有所增加, 且差异有统计学意义(P <0.05);与PSD 组比较药物组下丘脑室旁核c-fos 蛋白的表达减少, 且差异有统计学意义(P <0.01)。结论盐酸帕罗西汀可以改善PSD 大鼠抑郁行为, 其抗抑郁作用可能与下调下丘脑室旁核c-fos 基因表达有关。

关键词: 卒中后抑郁, 室旁核, 早期即刻基因, 盐酸帕罗西汀

Abstract: AIM: To explore the molecular action mechanism of antidepression drug on the poststroke depression (PSD) rats.METHODS: The PSD rat model of focal cerebral ischemia was established by threadtightening middle cerebral artery, followed with restraint stress and isolated breeding.The changes on rats's spontaneous behavior and the expression of c-fos in hypothalamus paraventricular nucleus (PVN), and the effects of paroxetine hydrochloride interference in the PSD rat model were evaluated.RESULTS: Compared with the control group, the consumption of sucrose water, the scores of upright and horizonal activities in the Open-Field test of PSD rats were decreased in significant differences(P <0.01).Compared with the PSD rats, the consumption of sucrose water, the scores of upright and horizonal activities in the drug treatment group increased in significant differences(P <0.01).Compared with the depression group or stroke group, the expressions of c-fos in PVN increased in PSD rats in significant differences (P <0.05).The expression of c-fos in PVN decreased in the drug treatment group, compared with the PSD group, in significant difference.CONCLUSION: The paroxetine could improve the PSD rats depression behavior, which may related with the c-fos decrease expression in the PVN.

Key words: poststroke depression, paraventricular nucleus, immediately-early genes, paroxetine hydrochloride

中图分类号:

R965.2

倪贵华, 邵蓓, 范红, 黄欢捷. 盐酸帕罗西汀对卒中后抑郁大鼠行为和下丘脑室旁核fos 蛋白表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(12): 1360-1364.

NI Gui-hua, SHAO Bei, FAN Hong, HUANG Huan-jie. Effects of paroxetine on the spontaneous behavior and the c-fos expression of hypothalamus paraventricular nucleus in poststroke depression rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1360-1364.

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