雌激素对卒中后抑郁大鼠血清单胺类递质的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (12): 1375-1380.

雌激素对卒中后抑郁大鼠血清单胺类递质的影响

王虹¹, 邵蓓¹, KevinO`Byrne², 李晓峰², 林源绍¹, 杨建荣³, 王志强⁴, 范虹⁵

¹温州医学院附属第一医院脑血管科,³检验科,⁴药剂科, 温州 325000, 浙江;
²英国伦敦国王学院, 伦敦 WC2R 2LS;
⁵绍兴市人民医院, 绍兴 312000, 浙江

收稿日期:2008-09-09 修回日期:2008-09-09 发布日期:2020-10-30
通讯作者: 邵蓓,女,教授,硕士生导师,主要从事脑血管病和神经电生理的研究。E-mail:shaobei@yahoo.com.cn
作者简介:王虹,女,硕士研究生,研究方向:脑血管病。E-mail:wanghong0710@126.com
基金资助:
温州市科技局对外合作重大项目(H2005B031)

Effect of estrogen on the monoamine neurotransmitter in the serum of rats with post-stroke depression

WANG Hong¹, SHAO Bei¹, Kevin O` Byrne², LI Xiao-feng², LIN Yuan-shao¹, YANG Jian-rong³, WANG Zhi-qiang⁴, FAN Hong⁵

¹Department of Cerebrovascular Disease, ³Department of Clinical Laboratory, ⁴Department of Pharmacy, the Affiliated No.1 Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China;
²King's College London, London, WC2R 2LS, the United Kingdom;
⁵People's Hospital of Shaoxing, Shaoxing 312000, Zhejiang, China

Received:2008-09-09 Revised:2008-09-09 Published:2020-10-30

摘要/Abstract

摘要： 目的探讨雌激素对卒中后抑郁(PSD) 大鼠的血清单胺类递质的影响。方法选用雌性SD大鼠, 经Open-Field 行为学评分后随机分成对照组、卒中组、PSD 组、药物治疗组和雌激素干预组,所有大鼠均行卵巢切除术。对照组为常规饲养;卒中组去卵巢后7 d 采用线栓法制备局灶性脑缺血大鼠模型;PSD 组为卒中大鼠结合孤养、束缚应激制备PSD 大鼠模型;药物治疗组对PSD 大鼠模型行帕罗西汀灌胃治疗;雌激素干预组对PSD 大鼠模型皮下包埋雌激素释放管。观察雌激素对PSD 大鼠自发性行为和外周血去甲肾上腺素(NE) 和5-羟色胺(5-HT) 的影响。结果与对照组比较, PSD 组大鼠旷场实验水平和垂直得分明显减少, 外周血NE 和5-HT 浓度明显降低, 差异有统计学意义(P <0.05)。与PSD 组比较, 雌激素干预组大鼠旷场实验得分增加, 外周血5-HT 和NE 浓度明显升高, 差异有统计学意义(P <0.05)。与药物治疗组相较, 雌激素干预组大鼠旷场实验得分、外周血5-HT 浓度差异无统计学意义(P >0.05)。结论雌激素能够改善PSD 大鼠的行为学、增加血清5-HT 浓度, 提示对PSD 大鼠有脑保护作用。

关键词: 雌激素, 卒中后抑郁, 去甲肾上腺素, 五羟色胺, 高效液相电化学法

Abstract: AIM: To study the effect of estrogen on the monoamine neurotransmitter in the serum of rats with post-stroke depression(PSD).METHODS: The female Sprague Dawley rats weighted 220-230 g were randomly divided into control group, stroke group, PSD group, antidepressant treatment group and estrogen treatment group according to the scores of Open-Field test.Rats in all groups were performed ovariectomy first.Rats in control group were feed commonly. Seven days later, rats in the stroke group, PSD group, drug treatment group and estrogen treatment group were all duplicated into focal ischemia by means of middle cerebral artery occlusion, then the rats in PSD group, drug treatment group and estrogen treatment group received isolated feed and restraint stress to duplicate PSD model.Rats in drug treatment group were received Paroxetine and in rats estrogen treatment group were received the E2-containing capsule that were embed in subcutaneous by operation.The E2-containing capsules produced circulating concentrations of E2 within the range observed during the diestrous phase of the estrous cycle.After that, the behavioral evaluations were done with Open-Field test, these rats were sacrificed and their blood were taken.The mononeurotransmitters included NE and 5-HT in serum were detected by HPLCECD.RESULTS: The spontaneous behavior and the concentration of NE and 5-HT in serum of the PSD rats decreased significantly in comparison with those in control group(P <0.05), the spontaneous behavior and the concentration of NE and 5-HT in serum of the rats in estrogen treatment group increased significantly in comparison with those in PSD group(P <0.05), the spontaneous behavior and the concentration of 5-HT in serum of the rats in estrogen treatment group increased significantly as those in drug treatment group (P > 0.05), the concentration of NE in estrogen treatment group increased non-significantly in comparison with that in drug treatment group(P <0.05).CONCLUSION: The spontaneous behavior could be improved and the 5-HT was increased by estrogen in PSD rats. That may protect the brain of PSD rats.

Key words: estrogen, post-stroke, depression, norepinephrine, 5-hydroxytryptamine, HPLC-ECD

中图分类号:

R965.2

王虹, 邵蓓, KevinO`Byrne, 李晓峰, 林源绍, 杨建荣, 王志强, 范虹. 雌激素对卒中后抑郁大鼠血清单胺类递质的影响[J]. 中国临床药理学与治疗学, 2008, 13(12): 1375-1380.

WANG Hong, SHAO Bei, Kevin O` Byrne, LI Xiao-feng, LIN Yuan-shao, YANG Jian-rong, WANG Zhi-qiang, FAN Hong. Effect of estrogen on the monoamine neurotransmitter in the serum of rats with post-stroke depression[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1375-1380.

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