灵芝多糖 硒化卡拉胶口服液对免疫抑制小鼠免疫功能的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (12): 1381-1387.

灵芝多糖硒化卡拉胶口服液对免疫抑制小鼠免疫功能的影响

张连龙¹, 戴敏², 毛兆祥¹, 吴欣², 张纯东², 周店云¹

¹无锡健特药业有限公司, 无锡 214091, 江苏;
²安徽中医学院药学院, 安徽省中药研究与开发重点实验室, 合肥 230038, 安徽

收稿日期:2008-09-11 修回日期:2008-12-08 发布日期:2020-10-30
作者简介:张连龙,男,硕士研究生,高级工程师,研究方向:保健食品的研制和分析测试。Tel:0510-85990008 E-mail:zhlianlong@sina.com

Effects of ganoderma liciudm ploysaccharide seleno-κ-carageenan syrup on immune function of immunosuppression mice

ZHANG Lian-long¹, DAI Min², MAO Zhao-xiang¹, WU Xin², ZHANG Chun-dong², ZHOU Dian-yun¹

¹Wuxi Giant Pharmaceutical CO., Ltd., Wuxi 214091, Jiangsu, China;
²College of Pharmacy, Anhui College of Traditional Chinese Medical, Anhui Key Laboratory of Modernized Chinese Material Medical, Hefei 230038, Anhui, China

Received:2008-09-11 Revised:2008-12-08 Published:2020-10-30

摘要/Abstract

摘要： 目的研究灵芝多糖硒化卡拉胶口服液对环磷酰胺诱导免疫抑制小鼠的非特异性免疫功能、体液免疫功能、细胞免疫功能的影响。方法小鼠腹腔注射80 mg/kg 环磷酰胺诱导免疫抑制小鼠模型, 灵芝多糖硒化卡拉胶口服液连续供应30 d, 每天1 次。测定小鼠单核巨噬细胞吞噬功能、腹腔巨噬细胞吞噬鸡红细胞功能、外周血白细胞数目、NK 细胞活性、白介素-1(IL^-1) 活性、白介素-2(IL-2) 活性、TNF-α活性、半数血清溶血素、抗体生成细胞、T 、B 淋巴细胞增殖能力、迟发型变态反应。结果灵芝多糖硒化卡拉胶口服液各剂量组(低剂量组:灵芝多糖2.5 mg/kg +硒5μg kg;中剂量组:灵芝多糖5 mg/kg +硒10μg kg;高剂量组:灵芝多糖15 mg/kg +硒30μg kg) 均可提高外周血白细胞数目、提高半数溶血素值, 低剂量组增强IL^-1 活性, 中剂量组增强IL-2 活性, 高剂量组和中剂量组增强T 、B 淋巴细胞的增殖能力、NK细胞活性、TNF-α活性, 高剂量组增强腹腔巨噬细胞吞噬功能、碳粒廓清值、提高抗体生成细胞、增强迟发型变态反应。结论灵芝多糖硒化卡拉胶口服液对免疫抑制小鼠的免疫功能具有改善作用。

关键词: 灵芝多糖硒化卡拉胶口服液, 非特异性

Abstract: AIM: To investigate the effects of ganoderma liciudm ploysaccharide (GLP) seleno-κ-carageenan syrups on nonspecific immunity, humoral immunity, cell immunity function of immune suppressed mice induced by cyclophosphamide.METHODS: Immunity hypofunction mouse models were established by intraperitoneal injection of cyclophosphamide (80 mg/kg, o.d.) for 3 days, and ganoderma liciudm ploysaccharide seleno-κ-carageenan syrups were administrated by gastric gavage for 30 days, once a day.The mononuclear phagocyte englobement function, the phagocytosis function(chicken erythrocyte) of abdominal cavity phagocyte, the number of peripheral blood leucocyte, NK cytoactive, interleukin-1 and interleukin-2 activity, TNF-αactivity, serum hemolysin, antibodyproducing cells, increment abilities of T, B lymphocyte delayed allergy were detected.RESULTS: The number of leukocytes, serum hemolysin increased in all 3 groups of ganoderma liciudm ploysaccharide seleno-κ-carageenan syrups(lower dose group:GLP 2.5 mg/kg +Se 5μg kg, medium dose group:GLP 5 mg/kg +Se 10μg kg, high dose group:GLP 15 mg/kg +Se 30μg kg).The activities of IL^-1 in lower dose group, and IL-2 in medium dose group increased.The reproductive activities of T, B lymphocytic cells, NK cytoactive, activity of TNF-αincreased in high group as well as the englobement function of peritoneal macrophage, the carbon particle clearance, antibody-producing cells, delayed allergy.The reproductive activities of T, B lymphocytic cells, NK cytoactive, activity of TNF-αincreased in medium group.CONCLUSION: Ganoderma Liciudm Ploysaccharide seleno-κ-carageenan syrups could improve the immunological function of immunosuppression mice.

Key words: ganoderma liciudm polysaccharide seleno-κ-carageenan syrups, nonspecific immunity, humoral immunity, cell immunity

中图分类号:

R593

张连龙, 戴敏, 毛兆祥, 吴欣, 张纯东, 周店云. 灵芝多糖硒化卡拉胶口服液对免疫抑制小鼠免疫功能的影响[J]. 中国临床药理学与治疗学, 2008, 13(12): 1381-1387.

ZHANG Lian-long, DAI Min, MAO Zhao-xiang, WU Xin, ZHANG Chun-dong, ZHOU Dian-yun. Effects of ganoderma liciudm ploysaccharide seleno-κ-carageenan syrup on immune function of immunosuppression mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1381-1387.

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