苦瓜蛋白对A549 肺癌细胞的抑制作用及其对MAPK 信号通路的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (12): 1396-1400.

苦瓜蛋白对A₅₄₉ 肺癌细胞的抑制作用及其对MAPK 信号通路的影响

韩义香¹, 李景荣², 章圣辉¹, 吴建波¹, 刘新华¹, 熊术道^1,3

¹温州医学院附属第一医院医科所;
²急诊科, 温州 325000, 浙江;
³复旦大学上海医学院免疫学系, 上海 200032

收稿日期:2008-09-12 修回日期:2008-10-07 发布日期:2020-10-30
通讯作者: 熊术道,男,助理研究员,主要从事肿瘤分子生物学及肿瘤免疫治疗研究。E-mail:xshdao@126.com
基金资助:
浙江省教育厅资助课题(20061779);浙江省温州市科技计划项目(Y20070051)

Inhibitory action of momordica on non-small cell lung cancer A₅₄₉ cells and its effects on MAPK signaling pathway

HAN Yi-xiang¹, Xing-rong², ZHANG Sheng-hui¹, WU Jian-bo¹, LIU Xin-Hua¹, XIONG Shudao^1,3

¹Institute of Medical Science, ²Department of Emergency, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China;
³Department of Immunology, Shanghai Medical College, Fudan University, Shanghai 200023, China

Received:2008-09-12 Revised:2008-10-07 Published:2020-10-30

摘要/Abstract

摘要： 目的观察苦瓜蛋白对非小细胞肺癌A₅₄₉细胞的生长抑制及促凋亡作用;探讨苦瓜蛋白对A₅₄₉ 细胞p42/44-MAPK 及其磷酸化p42/44-MAPK蛋白表达水平的影响及其作用分子机制。方法以不同浓度梯度的苦瓜蛋白处理A₅₄₉ 细胞, 分别作用不同时间,CCK-8 检测其对细胞生长抑制作用;流式细胞术检测细胞凋亡。提取苦瓜蛋白不同作用时间点的全细胞蛋白, western blot 检测p42/44-MAPK 及p-p42/44-MAPK 信号分子的变化。结果苦瓜蛋白对A₅₄₉ 细胞生长具有明显抑制作用, 可显著地诱导A₅₄₉ 细胞凋亡。与阴性对照比较, 苦瓜蛋白可以不同程度下调p42/44-MAPK 及p-p42/44-MAPK 信号分子的表达(P <0.05)。结论苦瓜蛋白对非小细胞肺癌A₅₄₉ 细胞具有明显地抑制作用及抗肿瘤作用, 其作用可能通过诱导A₅₄₉ 细胞凋亡来实现;苦瓜蛋白可以不同程度地下调p42/44-MAPK 和磷酸化p42/44-MAPK 的表达;MAPK 信号通路可能部分参与了苦瓜蛋白诱导肿瘤细胞凋亡的分子机制, 驱使肿瘤细胞发生凋亡。

关键词: 苦瓜蛋白, A₅₄₉ 细胞, 细胞凋亡, MAPK 通路

Abstract: AIM: To observe the inhibitory action and apoptotic activity of momordin to nonsmall-cell lung cancer A₅₄₉ cells and explore its effects on the expression of p42/44-MAPK and phosphorylation pp42/44-MAPK and its mechanisms of action.METHODS: A₅₄₉ cells were treated with different concentration gradient of momordinand and incubated for different time, then the cytostatic activity and apoptosis to A₅₄₉ cells were detected by CCK-8 kit and flow cytometry, respectively.The bitter melon protein were extracted for different time and the changes of signaling molecules such as p42/44-MAPK and p-p42/44-MAPK were detected by western blot.RESULTS: CCK-8 test indicated that momordin had a strong cytostatic activity to A₅₄₉ cells.TUNEL fluorescent assay indicated that momordin could markedly induce A₅₄₉ cells apoptosis. Western blot assay showed that the expression of p42/44-MAPK and p-p42/44-MAPK were partially down regulated compared with the negative control group (P <0.05).CONCLUSION: Momordin has a strong cytostatic and anti-tumor activity to non-small-cell lung cancer A₅₄₉ cells through inducing A₅₄₉ cells apoptosis.Its partial mechanisms maybe involve in inhibiting the MAPK signal pathway such as down-regulating the expression of p42/44-MAPK and phosphorylation p42/44-MAPK and driving tumor cells to apoptosis.

Key words: momordin, A₅₄₉ cell, apoptosis, p42/44-MAPK, p-p42/44-MAPK

中图分类号:

韩义香, 李景荣, 章圣辉, 吴建波, 刘新华, 熊术道. 苦瓜蛋白对A₅₄₉ 肺癌细胞的抑制作用及其对MAPK 信号通路的影响[J]. 中国临床药理学与治疗学, 2008, 13(12): 1396-1400.

HAN Yi-xiang, Xing-rong, ZHANG Sheng-hui, WU Jian-bo, LIU Xin-Hua, XIONG Shudao. Inhibitory action of momordica on non-small cell lung cancer A₅₄₉ cells and its effects on MAPK signaling pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1396-1400.

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苦瓜蛋白对A₅₄₉ 肺癌细胞的抑制作用及其对MAPK 信号通路的影响

Inhibitory action of momordica on non-small cell lung cancer A₅₄₉ cells and its effects on MAPK signaling pathway

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