雷公藤红素对小鼠的免疫抑制作用及其对IL-6 mRNA 表达影响的研究

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (2): 158-163.

雷公藤红素对小鼠的免疫抑制作用及其对IL-6 mRNA 表达影响的研究

李孟秋¹, 窦洁¹, 杜伟¹, 李新亚², 林健², 周长林¹

¹中国药科大学生命科学与技术学院, 南京 210009, 江苏;
²江苏美通制药有限公司, 泰州 225300, 江苏

收稿日期:2007-11-17 修回日期:2008-01-07 出版日期:2008-02-26 发布日期:2020-10-14
通讯作者: 周长林, 男, 教授, 硕导, 主要从事生化药学研究。Tel:025-83271323 E-mail:cl-zhou@cpu.edu.cn
作者简介:李孟秋, 女, 硕士, 主要研究方向:生化药学。Tel:025-83271323 　E-mail:limengqiu0918@sina.com

Effects of tripterine on the immunosuppression action and expression of IL-6 mRNA in mice

LI Meng-qiu¹, DOU Jie¹, DU Wei¹, LI Xin-ya², LIN Jian², ZHOU Chang-lin¹

¹School of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China;
²Jiangsu Meitong Pharmaceutical Co., LTD. Taizhou 225300, Jiangsu, China

Received:2007-11-17 Revised:2008-01-07 Online:2008-02-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:研究雷公藤红素对小鼠的免疫抑制作用及对细胞因子IL-6 mRNA 表达的影响。方法:采用碳粒廓清、迟发型变态反应、血清溶血素测定和T 淋巴细胞转化实验, 观察雷公藤红素对小鼠免疫功能的影响;运用RT-PCR 半定量法研究雷公藤红素对小鼠肝脏IL-6 mRNA 表达影响。结果:雷公藤红素能抑制小鼠血清溶血素水平(400 μg/kg), 且与剂量呈依赖关系;小鼠耳廓肿胀度研究表明, 雷公藤红素能使迟发型变态反应程度减轻(400 μg/kg );雷公藤红素剂量在0.25 μg/mL 时, 可以明显抑制植物血凝素(PHA)诱导的细胞增殖;雷公藤红素800 μg/kg 能对抗CCl₄ 引起的急性肝损伤小鼠肝脏IL-6 mRNA 含量的升高。结论:雷公藤红素在一定程度上能抑制小鼠的免疫功能, 能抑制炎症细胞因子IL-6 基因的过度表达。

关键词: 雷公藤红素, 免疫抑制, IL-6, mRNA 表达

Abstract: AIM:To investigate the effects of tripterine on the immunosuppression action and the over expression of IL-6 gene in mice.METHODS:The carbon particle clearance reaction test, delayed hyper-sensitivity reaction test, serum hemolysin assay and lymphocyte transfer test were applied to observe the immunosuppression action of tripterine.Mice were orally pretreated with tripterine, and the IL-6 mRNA level was determined by RT-PCR.RESULTS:The level of serum hemolsin in mice treated with triperine (400 μg/kg) was much lower than that of control group, suggesting that tripterine may suppress the level of serum hemolsin in mice in dose dependent manner.Tripterine (400 μg/kg) had an obvious effect of immunosuppression on mice cells by relieving the delayed hypersensitivity reaction, and tripterine (0.25 μg/mL) had inhibitory effects on the activation and proliferation of mice T lymphocytes stimulated by PHA.In addition, significant increase of IL-6 mRNA level induced by CCl₄ was remarkably decreased by 800 μg/kg tripterine.CONCLUSION:Tripterine suppresses the immunological function of mice to some extent and inhibits the over expression of IL-6 gene in mice liver which is related to proinflammatory cytokines.

Key words: tripterine, immunosuppression, IL-6, expression of mRNA

中图分类号:

R963
R285.5

李孟秋, 窦洁, 杜伟, 李新亚, 林健, 周长林. 雷公藤红素对小鼠的免疫抑制作用及其对IL-6 mRNA 表达影响的研究[J]. 中国临床药理学与治疗学, 2008, 13(2): 158-163.

LI Meng-qiu, DOU Jie, DU Wei, LI Xin-ya, LIN Jian, ZHOU Chang-lin. Effects of tripterine on the immunosuppression action and expression of IL-6 mRNA in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(2): 158-163.

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