黄芩苷对动脉粥样硬化家兔的保护作用及其机制

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (2): 188-194.

黄芩苷对动脉粥样硬化家兔的保护作用及其机制

马珺, 吴晓冬

东南大学基础医学院药理教研室, 南京 210009, 江苏

收稿日期:2007-12-07 修回日期:2008-01-11 出版日期:2008-02-26 发布日期:2020-10-14
通讯作者: 吴晓冬, 男, 博士, 副教授, 硕士生导师, 研究方向:心血管药理学。Tel:13073489711 E-mail:robertwxd@yahoo.com.cn
作者简介:马珺, 女, 硕士研究生, 研究方向:心血管药理学。Tel:13851694856 E-mail:mljz0211@yahoo.com.cn

Effects and possible mechanisms of baicalin on the atherosclerosis rabbits

MA Jun, WU Xiao-dong

Department of Pharmacology, Medical School, Southeast University, Nanjing 210009, Jiangsu, China

Received:2007-12-07 Revised:2008-01-11 Online:2008-02-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:探讨黄芩苷对动脉粥样硬化(AS) 家兔的保护作用及可能机制。方法:新西兰白兔30只, 随机分为正常组、模型组、黄芩苷治疗组(300 mg/kg) 、黄芩苷预防组(100 mg/kg) 、阳性药(辛伐他汀) 对照组, 每组6 只。酶法测定各组血清及肝脏中TC 、TG 、低密度脂蛋白(LDL) 、高密度脂蛋白含量, 主动脉苏丹Ⅳ染色测AS 斑块面积/总面积, HE 染色观察主动脉及肝脏形态学改变。ELASA 方法测血清中肿瘤坏死因子(TNF-α) 、白细胞介素-1β(IL-1β) 、脂联素(Adiponectin) 的水平。免疫组化法检测主动脉弓处的NF-κB 的表达。结果:黄芩苷治疗组、黄芩苷预防组、阳性药对照组主动脉粥样硬化斑块面积小于模型组(P <0.01), 黄芩苷治疗组、黄芩苷预防组、阳性药对照组血清及肝脏中TC 、LDL 水平较模型组明显降低(P <0.01), 黄芩苷治疗组、黄芩苷预防组血清中TNF-α、IL-1β 和脂联素水平较模型组明显降低(P<0.01, P <0.05), 黄芩苷治疗组、黄芩苷预防组主动脉壁上NF-κB 的表达低于模型组(P <0.01) 。结论:黄芩苷通过升高脂联素、下调NF-κB 抑制炎症反应及调节血脂两个方面对AS 起保护作用, 这可能是其抑制AS 的形成及发展的重要机制之一。

关键词: 动脉粥样硬化, 黄芩苷, 脂联素, 核因子-κB, 肿瘤坏死因子-α, 白细胞介素-1β

Abstract: AIM:To investigate the effects and possible mechanisms of baicalin on the atherosclerosis rabbits.METHODS:Thirty healthy New Zealand white rabbits were divided randomly into five groups: normal control group (A group, n =6) and fed with normal diet, atherosclerosis model group(B group, n =6), baicalin therapic group(C group, n =6), bacalin preventive group(D group, n =6), and positive control group(E group, n =6), the last four groups fed with hypercholesterol diet for 12 weeks.Treated with bacalin 300 mg/(kg·d), and simvastatin 5 mg/ (kg·d) additionally for 2 weeks in C and E group respectively from the tenth week.Treat with bacalin 100 mg/(kg·d) for 12 weeks in D group.Serum lipids and liver lipids were detected with standard enzymatic assays.Atherosclerotic plaque/intima size ratio of area and NF-κB content in blood vessel tissue were examined.Enzyme-linked immunosorbent assay (ELISA) was employed to monitor the levels of serum TNF-α, IL-1β and Adiponectin.RESULTS:Atherosclerotic plaque intima size ratio of area decreased in C, D and E group than in B group(P <0.01).The levels of Blood lipids, liver lipids decreased in C, D and E group than in B group(P <0.01).Serum TNF-α, IL- 1β, and adiponectin decreased in C and D group than in B group(P <0.01, P <0.05).The NF-κB activity in C and D group were lower than B group(P <0.01). CONCLUSION:Baicalin improve atherosclerosis not only by decreasing blood lipids, but also by anti-inflammation through increasing serum levels of adiponectin, decreasing activity of NF-κB in atherosclerotic focus and serum levels of TNF-α, IL-1β.

Key words: atherosclerosis, baicalin, adiponectin, NF-κB, TNF-α, IL-1β

中图分类号:

R965.2

马珺, 吴晓冬. 黄芩苷对动脉粥样硬化家兔的保护作用及其机制[J]. 中国临床药理学与治疗学, 2008, 13(2): 188-194.

MA Jun, WU Xiao-dong. Effects and possible mechanisms of baicalin on the atherosclerosis rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(2): 188-194.

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