羟基脲对大鼠Sertoli-germ 细胞共培养的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (5): 512-516.

羟基脲对大鼠Sertoli-germ 细胞共培养的影响

周莉¹, 吴纯启², 杨保华², 荆淑芳², 廖明阳²

¹中国医学科学院实验动物研究所新药安全评价研究中心, 北京100050;
²军事医学科学院毒物药物研究所国家(北京) 药物安全评价研究中心, 北京100850

收稿日期:2008-05-22 修回日期:2008-06-10 发布日期:2020-11-09
通讯作者: 廖明阳, 男, 研究员, 博士生导师, 从事药物毒理学和新药评价研究。Tel:010-66931628 E-mail:liaomingy @hotmail. com
作者简介:周莉, 女, 博士, 助研, 从事生殖毒理研究。Tel:010-63031045 E-mail:btzhl96 @sina.com
基金资助:
国家高新技术研究发展计划(863 计划, 2002AA2Z342D)

Effect of hydroxyurea on rat Sertoli- germ cell Co-culture

ZHOU li¹, WU Chun-qi², YANG Bao-hua², JING Shu-fang², LIAO Ming-yang²

¹Chinese Academy of Medical Sciences, Institute of Laboratory Sciences, Center for Safety Evaluation and Research of Drug, Beijing 100050, China;
²National (Beijing) Center for Drug Safety Evaluation and Research, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China

Received:2008-05-22 Revised:2008-06-10 Published:2020-11-09

摘要/Abstract

摘要： 目的:观察羟基脲(Hydroxyurea, HU) 对雄性大鼠Sertoli-germ 细胞共培养的细胞毒性作用。方法:在Sertoli-germ 细胞共培养模型中分别加入不同浓度HU(0、 10^-3、 10^-2、 10^-1 mol/L) 后, 孵育96 h; 以及加入10^-2 mol/L HU 后分别孵育24、 48、 72、 96 h 。结果:在各给药组, 96 h 时HU 导致germ细胞从Sertoli 细胞层的脱落增加, 呈现量效关系。在10^-3 mol/L 以上浓度组, 细胞活性逐渐下降, 细胞脱落增加。同样, 随着给药时间的增加, germ细胞脱落率和乳酸脱氢酶(LDH) 的漏出逐渐增加, 细胞活性逐渐下降。结论:HU 对雄性大鼠睾丸Sertoli-germ 细胞共培养模型的germ 细胞具有明显毒性作用。

关键词: 羟基脲, Sertoli-germ 细胞, 共同培养技术, 细胞毒性

Abstract: AIM:to evaluate the cytotoxic effects of Hydroxyurea (HU) in vitro employing rat testicular Sertoli-germ cell coculture. METHODS:Cytotoxic changes induced by HU (0, 10^-3, 10^-2, 10^-1mol/L)in mixed cultures of Sertoli and germ cells were seen at 96 h after treatment, respectively. And 10^-2 mol/L HU were added to the culture, at 24, 48, 72 and 96 h after treatment, respectively. RESULTS:HU led to an increase in germ cell detachment from the Sertoli cell monolayer in a dose dependent manner. Detached cells were observed and the viability of detached cells was decreased in 10^-3 mol/L and above treated groups. Substantial leakage of the cytosolic enzyme lactate dehydrogenase (LDH) was observed in the medium after 96 h HU treatment at concentrations of 10^-2mol/L and above treated groups. The same, HU exposure led to an increase in germ cell detachment, the leakage of LDH, a decrease in cell viability from the Sertoli cell monolayer in a time dependent manner compared with the control culture. CONCLUSION:It is shown that HU exhibited an obvious toxicity to the germ cells of male rats in Sertoli cell germ cell co-culture model.

Key words: hydroxyurea, Sertoli-germ cell, coculture, cytotoxity

中图分类号:

R114

周莉, 吴纯启, 杨保华, 荆淑芳, 廖明阳. 羟基脲对大鼠Sertoli-germ 细胞共培养的影响[J]. 中国临床药理学与治疗学, 2008, 13(5): 512-516.

ZHOU li, WU Chun-qi, YANG Bao-hua, JING Shu-fang, LIAO Ming-yang. Effect of hydroxyurea on rat Sertoli- germ cell Co-culture[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(5): 512-516.

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