雷帕霉素抑制增生内膜中基质细胞衍生因子-1的表达

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (6): 644-647.

雷帕霉素抑制增生内膜中基质细胞衍生因子-1的表达

雷建军, 夏艺萍, 胡艳, 王佐, 龙光, 危当恒

南华大学心血管疾病研究所, 动脉硬化学湖南省重点实验室, 衡阳 421001, 湖南

收稿日期:2008-05-27 修回日期:2008-06-30 发布日期:2020-10-14
通讯作者: 危当恒,男,博士,副教授,研究方向:动脉粥样硬化发病机制及其防治。 E-mail:weizhonghua99@126.com
作者简介:雷建军,男,硕士,助理实验师,研究方向:血管内膜增生的发病机制及其防治。Tel:0734-6685532 E-mail:leijianjunhu@163.com
基金资助:
湖南省自然科学基金项目(06JJ50051, 06JJ30016);湖南省教育厅科学研究项目(06C693)

Inhibitory effect of rapamycin on stromal cell-derived factor-1 expression in intimal hyperplasia

LEI Jian-jun, XIA Yi-ping, HU Yan, WANG Zuo, LONG Guang, WEI Dang-heng

Insititue of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan Province, University of South China, Hengyang 421001, Hunan, China

Received:2008-05-27 Revised:2008-06-30 Published:2020-10-14

摘要/Abstract

摘要： 目的: 进一步探讨雷帕霉素抗血管内再狭窄的分子机制。方法: 球囊拉伤大鼠颈总动脉建立血管内膜增生的动物模型,口服雷帕霉素(25 mg/kg),4周后处死动物。HE染色观测血管病变,免疫组化检测增生内膜中基质细胞衍生因子-1(SDF-1)的表达,小室迁移系统观察SDF-1对平滑肌细胞迁移的影响。结果: 球囊拉伤明显诱导血管内膜增生,增生内膜中有明显的SDF-1的表达,雷帕霉素能显著下调增生内膜中SDF-1的表达,而SDF-1浓度依赖性地诱导平滑肌细胞的迁移。结论: 雷帕霉素可能通过下调SDF-1的表达从而抑制血管再狭窄。

关键词: 雷帕霉素, 内膜增生, 基质细胞衍生因子-1(SDF-1), 球囊拉伤

Abstract: AIM: To investigate the anti-restenosis molecular mechanism of rapamycin.METHODS: The animal models of rat carotid artery intimal hyperplasia was established with Balloon injury.Rats were randomized divided into two groups: baseline group (n=10) and treatment group (n=10 rapamycin, 25 mg/kg). HE staining was used to observe vascular lesions.Immunochistochemistry staining was used to detect the expression of SDF-1 in lesions.The migration of smooth muscle cells induced by stromal cell-derived factor 1 (SDF-1) was observed with transwell.RESULTS: Intimal hyperplasia was obviously induced by balloon injury.SDF-1 highly expressed in lesions and rapamycin significantly reduced the expression of SDF-1 in intimal hyperplasia.SDF-1 induced the migration of smooth muscle cells with concentration-dependent manner.CONCLUSION: Rapamycin may inhibit vascular restenosis by inhibiting the migration of smooth muscle cells induced by SDF-1.

Key words: rapamycin, intimal hyperplasia, stromal cell-derived factor-1(SDF-1), balloon injury

中图分类号:

R965.1

雷建军, 夏艺萍, 胡艳, 王佐, 龙光, 危当恒. 雷帕霉素抑制增生内膜中基质细胞衍生因子-1的表达[J]. 中国临床药理学与治疗学, 2008, 13(6): 644-647.

LEI Jian-jun, XIA Yi-ping, HU Yan, WANG Zuo, LONG Guang, WEI Dang-heng. Inhibitory effect of rapamycin on stromal cell-derived factor-1 expression in intimal hyperplasia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(6): 644-647.

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