参附对大鼠肾缺血再灌注损伤NF-κB、细胞黏附分子-1、TNF-α表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (9): 1005-1009.

参附对大鼠肾缺血再灌注损伤NF-κB、细胞黏附分子-1、TNF-α表达的影响

孙艳玲¹, 吴五洲², 刘先义³, 秦成名¹

¹郧阳医学院附属太和医院麻醉科,²脊柱外科, 十堰442000, 湖北;
³武汉大学人民医院麻醉科, 武汉430060, 湖北

收稿日期:2008-06-11 修回日期:2008-09-14 出版日期:2008-09-26 发布日期:2020-10-13
通讯作者: 刘先义, 男, 主任医师、教授, 硕士生导师, 研究方向:器官保护。Tel:13507117487 E-mail:whxyl2005@126.com
作者简介:孙艳玲, 女, 医学硕士、主治医师, 讲师, 主要从事麻醉与器官保护工作。Tel:13872809376 E-mail:sysyl2005@126.com

Effects of shenfu injection on expression of NF-κB, TNF-αand ICAM-1 after renal ischemia/reperfusion in rats

SUN Yan-ling¹, WU Wu-zhou², LIU Xian-yi³, QING Cheng-ming¹

¹Department of Anesthesiology,²Department of Orthopedics, Taihe Hospital, Yunyang Medical College, Shiyan 442000, Hubei, China;
³Department of Anesthesiology, people's Hospital of Wuhan University, Wuhan 430060, Hubei, China

Received:2008-06-11 Revised:2008-09-14 Online:2008-09-26 Published:2020-10-13

摘要/Abstract

摘要： 目的:观察参附注射液(shenfu injection, SF)对大鼠肾缺血再灌注损伤细胞黏附分子-1(ICAM-1)、NF-κB、TNF-α表达的影响。方法:采用切除右肾, 无创动脉夹夹闭左肾动脉45 min, 再灌注2 h制作在体肾缺血再灌注损伤模型。36 只SD 大鼠随机分为缺血再灌注组、假手术对照组、参附预处理组(10 mL/kg), 每组12 只。免疫组化法检测肾组织中NF-κB、ICAM-1 蛋白含量, 酶联免疫吸附实验(ELISA) 检测血清、肾组织中TNF-α的表达。结果:缺血再灌注组NF-κB、ICAM-1 表达和TNF-α含量明显高于假手术对照组(P<0.01) 。与缺血再灌注组相比, 参附预处理组NF-κB、ICAM-1 表达和TNF-α含量明显降低(P<0.01) 。结论:参附通过抑制NF-κB 的表达, 从而下调其下游的TNF-α、ICAM-1 的表达, 发挥其肾脏保护作用。

关键词: 缺血再灌注, 参附注射液, 核因子-κB, 细胞间黏附分子-1, 肿瘤坏死因子-α

Abstract: AIM: To explore the effect of shenfu injection on renal ischemia/reperfusion injury in rats and to observe the expression of nuclear factor kappa B (NF-κB), tumour necrosis factor-α (TNF-α) and intercellular adhension molecule-1 (ICAM-1).METHODS: 36 SD rats were divided into three groups randomly.There were 12 rats in each group.Renal ischemia/reperfusion injury was induced by left renal pedicle occlusion for 45 minutes, followed by reperfusion with contralateral nephrectomy in rats.In shenfu pretreatment group, the rats were pretreated 1 hour before ischemia with shenfu injection.In ischemia/reperfusion group and control group, the animals were both pretreated with 0.9% natrill chloride, but in control group, the left renal pedicle was isolated, however, occlusion of the pedicle was not performed.The blood and kidney tissue were harvested after 2 hours of reperfusion.The serum creatinine (Cr) and blood urea nitrogen (BUN) values were measured with an automatic analyzer.Immunohistochemistry were used to evaluated the expression levels of NF-κB and ICAM-1, the concentration of tumor necrosis factor-α (TNF-α) in the kidney and plasma was determined by enzyme-linked immunoadsordent assay (ELISA).RESULTS: Compared with control group, BUN and Cr were gradually increased in ischemia/reperfusion group (P<0.01).The average OD values of NF-κB and ICAM-1 protein were significantly higher in ischemia/reperfusion group than those in shenfu pretreatment group (P<0.01) and control group (P<0.01).The levels of TNF-α in the kidney tissue and plasma were markedly increased after reperfusion.By contrast, the levels of TNF-α in shenfu pretreatment group were significiantly lower than those in ischemia/reperfusion group (P<0.05).CONCLUSION: The results show that SF has a protective effect against renal ischemia/reperfusion injury, which may through inhibiting the activity of NF-κB and the expression of ICAM-1 and TNF-α.

Key words: ischemia/reperfusioninjury, shenfuinjection, nuclearfactorkappaB, intercellularadhensionmolecule-1, tumornecrosisfactor-α

中图分类号:

R966

孙艳玲, 吴五洲, 刘先义, 秦成名. 参附对大鼠肾缺血再灌注损伤NF-κB、细胞黏附分子-1、TNF-α表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(9): 1005-1009.

SUN Yan-ling, WU Wu-zhou, LIU Xian-yi, QING Cheng-ming. Effects of shenfu injection on expression of NF-κB, TNF-αand ICAM-1 after renal ischemia/reperfusion in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(9): 1005-1009.

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