CpG 基序的寡脱氧核苷酸对人轮状病毒乳鼠腹泻模型的保护作用

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (9): 1010-1016.

CpG 基序的寡脱氧核苷酸对人轮状病毒乳鼠腹泻模型的保护作用

徐志伟, 李孟荣, 陈益平, 石海矾, 狄军波

温州医学院附属育英儿童医院感染科, 温州325027, 浙江

收稿日期:2008-06-30 修回日期:2008-07-27 出版日期:2008-09-26 发布日期:2020-10-13
作者简介:徐志伟, 男, 副主任医师, 研究方向:小儿感染性疾病。Tel:0577-88816173 E-mail:xuzw1971@sina.com

Protection of CpG oligodeoxynucleotide on human rotavirus-induced diarrhea in suckling mouse model

XU Zhi-wei, LI Meng-rong, CHEN Yi-ping, SHI Hai-fan, DI Jun-bo

Department of Infection, Yuying Children's Hospital Affiliated to WenzhouMedical College, Wenzhou 325027, Zhejiang, China

Received:2008-06-30 Revised:2008-07-27 Online:2008-09-26 Published:2020-10-13

摘要/Abstract

摘要： 目的:探讨含CpG 基序的寡脱氧核苷酸(CpG oligodeoxynucleotide,CpG ODN)干预对人轮状病毒(human rotavirus,HRV)感染乳鼠的保护作用机制。方法:64 只ICR 乳鼠随母鼠按窝随机均分为4 组:正常对照组、HRV 感染组、CpG ODN 预处理组、CpG ODN 治疗组。观察各组乳鼠的治疗反应和排毒情况;免疫组化法检测小肠组织Toll 样受体9(Toll-like receptor 9, TLR9)、IFN-γ的表达。结果:HRV 感染组乳鼠的腹泻天数、排毒时间及重型腹泻发病率均高于CpG ODN 预处理组和CpG ODN 治疗组(P<0.05);CpG ODN 预处理组、CpG ODN 治疗组TLR9、IFN-γ表达水平明显高于正常对照组和HRV 感染组(P<0.01), 并且两者存在正相关(P<0.05)。结论:CpG ODN 预处理和治疗都能提高HRV 感染乳鼠小肠组织TLR9、IFN-γ的表达, 对HRV 感染乳鼠有保护作用。

关键词: CpG 基序的寡脱氧核苷酸, 轮状病毒, Toll样受体9, IFN-γ

Abstract: AIM: To explore the protectionmechanism of CpG oligodeoxynucleotide (CpG ODN) on Human rotavirus diarrhea in suckling mice.METHODS: 64 ICR suckling mice were randomly divided into control group (A), HRV infection group (B), CpG ODN pretreatment group (C) and CpG ODN treatment group (D).The treatment response was observed and RV antigen in animal stools was detected.The expressions of Toll-like receptor 9 (TLR9) and interferon-gamma (IFN-γ) in the intestinal tissue were measured with immunohistochemistry.RESULTS: The duration of diarrhea, RV excretion time and the incidence of severe diarrhea of group B were higher than those in group C and group D (P<0.05).The expressions of TLR9, IFN-γ of group C and group D were higher than those of group A and group B (P<0.01), there were a positive correlation in the expression of TLR9 and IFN-γ of group C and group D (P<0.05).CONCLUSION: CpG ODN pretreatment and treatment can improve the expression of TLR9 and IFN-γin intestinal epithelium, CpG ODN has protection to HRV infected suckling mouse.

Key words: CpG oligodeoxynucleotide, rotavirus, TLR9, IFN-γ

中图分类号:

R965.2

徐志伟, 李孟荣, 陈益平, 石海矾, 狄军波. CpG 基序的寡脱氧核苷酸对人轮状病毒乳鼠腹泻模型的保护作用[J]. 中国临床药理学与治疗学, 2008, 13(9): 1010-1016.

XU Zhi-wei, LI Meng-rong, CHEN Yi-ping, SHI Hai-fan, DI Jun-bo. Protection of CpG oligodeoxynucleotide on human rotavirus-induced diarrhea in suckling mouse model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(9): 1010-1016.

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