大鼠相关脑区p-CREB 和c-Fos在吗啡点燃条件性位置偏爱条件下的变化

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (1): 10-13.

大鼠相关脑区p-CREB 和c-Fos在吗啡点燃条件性位置偏爱条件下的变化

赵永娜, 方正梅, 邵晓霞, 李晓红, 李顺英

昆明医学院药学院药理毒理学教研室, 昆明 650031, 云南

收稿日期:2008-08-07 修回日期:2008-12-31 出版日期:2009-01-26 发布日期:2020-10-27
作者简介:赵永娜, 女, 副教授, 主要研究方向:神经药理学。Tel:0871-5338904 　E-mail: mszhaoyn@yahoo.com.cn
基金资助:
云南省教育厅科学技术基金项目(03Y480C); 云南省应用基础研究计划面上项目(2006COO37Q)

Changes of p-CREB and c-Fos expression in brain regions of morphine-reactivated conditioned place preference in rats

ZHAO Yong-na, FANG Zheng-mei, SHAO Xiao-xia, LI Xiao-hong, LI Shun-ying

Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Kunming Medical College, Kunming 650031, Yunnan, China

Received:2008-08-07 Revised:2008-12-31 Online:2009-01-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 研究磷酸化cAMP 反应元件结合蛋白(phospho-cAMP response element binding protein,p-CREB) 和c-Fos 在吗啡点燃条件性位置偏爱激活大鼠海马、杏仁核表达的变化。方法: 以剂量递增连续皮下(s.c.) 注射吗啡6 d 建立吗啡诱导大鼠条件位置性偏爱(conditioned place preference,CPP) 模型, 第7 天用生理盐水替代吗啡训练大鼠10 d, 使CPP 消退, 单次s.c.吗啡(4 mg/kg) 激发已消退的CPP 。采用免疫组化技术测定吗啡激发CPP 重现时大鼠海马、杏仁核p-CREB 和c-Fos 的变化。结果: 吗啡可使大鼠产生CPP 效应, 吗啡4 mg/kg 可使已消失的CPP 效应激活;吗啡诱发CPP 激活时大鼠海马、杏仁核p-CREB 和c-Fos 的表达增加。结论: 海马、杏仁核p-CREB 和c-Fos蛋白的表达参与了吗啡点燃CPP 重现。

关键词: 吗啡, 条件性位置偏爱, 海马, 杏仁核, cAMP 反应元件结合蛋白, c-Fos

Abstract: AIM: To investigate the changes of phospho-cAMP response element binding protein (p-CREB) and c-Fos in two brain regions of morphine-reactivated conditioned place preference (CPP) in rats. METHODS: Morphine was administered by subcutaneous injection at gradually increasing dose for 6 days to establish morphine CPP.From the 7th day, the rats were administered saline instead of morphine for 10 days to induce CPP extinction.The rats were given a single priming injection of morphine to reactivate the morphine CPP.The p-CREB and c-Fos were assayed with immunohistochemistry method in the phase of recurrence of CPP rekindled by morphine.RESULTS: Increasing dose of morphine conditioning for 6 days resulted in acquisition of CPP and morphine (4 mg/kg) reactivated CPP following 10 days drug free period. Compared with saline control, morphine-reactivated CPP elevated the expression of p-CREB and c-Fos in hippocampus and amygdala in rats (P< 0.05).CONCLUSION: p-CREB and c-Fos expression in hippocampus and amygdala may be involved in the mechanisms of recurrence of CPP.

Key words: morphine, conditioned place preference, hippocampus, amygdala, cAMP response element binding protein, c-Fos

中图分类号:

R965.2

赵永娜, 方正梅, 邵晓霞, 李晓红, 李顺英. 大鼠相关脑区p-CREB 和c-Fos在吗啡点燃条件性位置偏爱条件下的变化[J]. 中国临床药理学与治疗学, 2009, 14(1): 10-13.

ZHAO Yong-na, FANG Zheng-mei, SHAO Xiao-xia, LI Xiao-hong, LI Shun-ying. Changes of p-CREB and c-Fos expression in brain regions of morphine-reactivated conditioned place preference in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 10-13.

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