HPLC-MS/MS 法与MEIA 法在监测器官移植患者他克莫司全血浓度中的比较

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (1): 80-83.

HPLC-MS/MS 法与MEIA 法在监测器官移植患者他克莫司全血浓度中的比较

李鹏飞, 刘丽宏, 马萍, 丁春雷, 童卫杭

中国人民解放军第二炮兵总医院药剂科, 北京 100088

收稿日期:2008-07-21 修回日期:2008-11-13 出版日期:2009-01-26 发布日期:2020-10-27
通讯作者: 刘丽宏,副教授, 硕士生导师, 从事医院药学工作。Tel:010-66343250 E-mail: hongllh@yahoo.com.cn
作者简介:李鹏飞, 药学硕士, 研究方向:体内药物分析。Tel:010-66343245 　E-mail:lee-pf@163.com

Comparison on two methods of HPLC-MS/MS and MEIA in monitoring the tacrolimus concentrations for organ transplantation patients

LI Peng-fei, LIU Li-hong, MA Ping, DING Chun-lei, TONG Wei-hang

Department of Pharmacy, the Second Artillery General Hospital, Beijing 100088, China

Received:2008-07-21 Revised:2008-11-13 Online:2009-01-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 比较HPLC-MS/MS 法与MEIA 法在监测器官移植患者他克莫司全血浓度中的应用。方法: 建立和确证可行的HPLC-MS/MS 法, 并与MEIA法分别测定他克莫司相同样本的浓度, 对测试结果进行统计分析, 评价两种方法。结果: HPLCMSMS 法测定他克莫司平均浓度为(4.86±0.46)ng/mL,MEIA 法测定浓度平均为(5.52±0.43)ng/mL, 两种方法测定结果相关系数r 平均为0.8771, 两种方法相关性较强。LC-MS/MS 法测定浓度值 MEIA 法测定浓度值平均值为(90.3±5.3)%。结论: HPLC-MS/MS 法测定浓度为他克莫司原型药物浓度, 能更准确地反映原型药物在体内的准确浓度, 更适用于日常治疗药物监测(TDM)工作。

关键词: 高效液相色谱-质谱联用法, 微粒子捕捉酶免疫发光技术, 器官移植, 他克莫司

Abstract: AIM: To compare two methods of HPLC-MS/MS and MEIA to monitor the tacrolimus concentrations for organ transplantation patient. METHODS: After developing and validating the HPLC-MS/MS method to determine the concentrations of tacrolimus, the tacrolimus samples were quantitated by two methods of HPLC-MS/MS and MEIA, respectively.Evaluate two methods by statistical analysis of quantitative results.RESULTS: The mean concentration were(4.86±0.46)ng/mL by HPLC-MS/MS and (5.52±0.43)ng/mL by MEIA.The coefficient correlation of two methods was 0.8771, and the two methods have good correlations in statistics.The mean concentration ratio was (90.3±5.3)%.CONCLUSION: The HPLC-MS/MS method is a more precise method for to determining the effective concentration of tacrolimus, which is suitable for daily TDM.

Key words: HPLC-MS/MS, microparticle enzyme immunoassay, organ transplantation, tacrolimus

中图分类号:

R969

李鹏飞, 刘丽宏, 马萍, 丁春雷, 童卫杭. HPLC-MS/MS 法与MEIA 法在监测器官移植患者他克莫司全血浓度中的比较[J]. 中国临床药理学与治疗学, 2009, 14(1): 80-83.

LI Peng-fei, LIU Li-hong, MA Ping, DING Chun-lei, TONG Wei-hang. Comparison on two methods of HPLC-MS/MS and MEIA in monitoring the tacrolimus concentrations for organ transplantation patients[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 80-83.

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