基于连接酶反应检测硫唑嘌呤基因多态性及应用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (11): 1281-1285.

基于连接酶反应检测硫唑嘌呤基因多态性及应用

梁欣^1,2, 张亚同², 程刚¹, 胡欣², 孙迎琪³, 杨晨¹, 李可欣², 孙春华²

¹沈阳药科大学药学院, 沈阳 100016, 辽宁;
²卫生部北京医院药学部, 北京 100730;
³北京大学药学院, 北京100191

收稿日期:2009-08-28 修回日期:2009-09-30 发布日期:2020-10-26
作者简介:梁欣, 女, 在读硕士, 研究方向:药物遗传、个体化治疗。Tel:15101035190 　E-mail:lx03106607@163.com；胡欣, 男, 博士, 主任药师, 硕士生导师, 研究方向:药物分析、药物遗传。Tel:010-85133636 　E-mai l:huxinbjyy@126.com
基金资助:
国家食品药品监督管理局药品评价中心国家科技支撑计划课题“安全用药关键技术与应用” 课题编号(2006BI14B04)

Establishment and application of ligase-based method detecting polymorphism of TPMT

LIANG Xin^1,2, ZHANG Ya-tong², CHENG Gang¹, HU Xin², SUN Ying-qi³, YANG Chen¹, LI Kexin², SUN Chun-hua²

¹Shenyang Pharmaceutical University, Shenyang 100016, Liaoning, China;
²Beijing Hospital, Ministry of Health,Beijing 100730, China;
³School of Pharmaceutical Science, Pecking University, Beijing 100191, Chnia

Received:2009-08-28 Revised:2009-09-30 Published:2020-10-26

摘要/Abstract

摘要： 目的：建立并探讨基于连接酶反应检测硫嘌呤甲基转移酶(thiopurine S-methyltransferase,TPMT) 基因分型分布的新方法。方法：建立多重PCR/连接酶检测方法检测TPMT 基因多态性, 并对50 份健康志愿者样本进行检测。结果：本方法可靠、迅速, 自动化程度高。研究未发现导致TPMT 酶活性降低的基因型^＊2,^＊3A,^＊3B 和^＊4,TPMT^＊3C 仅发现2 例杂合子。结论：本文建立的基因分型技术可为TPMT 基因型检测提供可靠的方法。

关键词: 硫嘌呤甲基转移酶, 连接酶检测反应, 硫唑嘌呤, 遗传多态性

Abstract: AIM: To establish one ligase-based method to detect the gene polymorphism of TPMT. METHODS: The multiplex PCR ligase detection reaction(LDR) was established, and 50 blood samples of healthy postulants were investigated with this method. RESULTS: This method was reliable, rapid, high-degree automatic.The genotype of ^＊2, ^＊3A, ^＊3B and ^＊4, which depressed the activity of TPMT were not found, only 2 samples of TPMT ^＊3C heterozygote were detected.CONCLUSION: This method can be applied for TPMT polymorphisms detection.

Key words: thiopurine S-methyltransferase, ligase detection reaction, azathiopurine, genetic polymorphism

中图分类号:

R965.2

梁欣, 张亚同, 程刚, 胡欣, 孙迎琪, 杨晨, 李可欣, 孙春华. 基于连接酶反应检测硫唑嘌呤基因多态性及应用[J]. 中国临床药理学与治疗学, 2009, 14(11): 1281-1285.

LIANG Xin, ZHANG Ya-tong, CHENG Gang, HU Xin, SUN Ying-qi, YANG Chen, LI Kexin, SUN Chun-hua. Establishment and application of ligase-based method detecting polymorphism of TPMT[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1281-1285.

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