OPG-HSP70 融合蛋白对CIA 大鼠的保护性作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (12): 1347-1350.

OPG-HSP70 融合蛋白对CIA 大鼠的保护性作用

刘姗, 余丹, 曾津津, 李慎涛, 王炜, 刘振龙, 赵文明

首都医科大学基础医学院免疫学系, 北京 100069

收稿日期:2009-10-30 修回日期:2009-12-11 发布日期:2020-10-20
作者简介:刘姗, 女, 硕士, 研究方向:自身免疫病。Tel:13911069465 E-mail:liushan200207@163.com
基金资助:
国家自然科学基金资助项目( 30873185); 北京市教委科技发展计划项目资助( KM200810025013)

Protective effects of OPG-HSP70 fusion protein on CIA rats

LIU Shan, YU Dan, ZENG Jin-jin, LI Shen-tao, WANG Wei, LIU Zhen-long, ZHAO Wen-ming

Department of Immunology, Capital Medical University, Beijing 100069, China

Received:2009-10-30 Revised:2009-12-11 Published:2020-10-20

摘要/Abstract

摘要： 目的：研究人骨保护素( OPG) -分枝杆菌热休克蛋白70(HSP70) 融合蛋白对类风湿关节炎动物模型———胶原诱导的关节炎( CIA) 大鼠的保护性作用。方法：将OPG-HSP70 重组基因菌株诱导表达, 收集蛋白并鉴定, 大量提取, 纯化, 复性;实验大鼠分为OPG-HSP70 组、OPG 组、HSP70 组、模型组和正常对照组, CIA 大鼠模型制备后分别给予相应处理, 观察对大鼠关节病变的影响。结果：与模型组相比, OPG-HSP70 组关节炎指数明显降低( P <0.05);关节病理切片显示关节滑膜无明显增生;骨密度值增高( P <0.05) 。结论：OPGHSP70融合蛋白具有减轻CIA 大鼠关节病变的作用。

关键词: 骨保护素, 热休克蛋白, 融合蛋白, 胶原诱导的关节炎

Abstract: AIM: To study the protective effects of human osteoprotegerin ( OPG) -Mycobacterium tuberculosis heat shock protein 70 ( HSP70) fusion protein on animal models of the rheumatoid arthritis by the observation of the symptoms of collagen-induced arthritis (CIA) rats.METHODS: The OPG-HSP70 recombinant strain stored in the laboratory were induced by isopropyl B-D-thiogalactopyranoside ( IPTG) and the acquired fusion protein were collected and identified, purificated and dialysis refolded.The experimental rats were divided into OPG-HSP70 group, OPG group, HSP70 group, model group and normal group, and were treated with corresponding protein after preparation.The symptoms and signs of collagen-induced arthritis rats were observed carefully.RESULTS: Compared with the positive control group, the OPG-HSP70 fusion protein group demonstrated that the arthritis index was reduced significantly ( P <0.05);the clear structures of the intra-articular synovials and the bone mineral density were increased ( P <0.05) from articular pathology studies.CONCLUSION: OPG-HSP70 fusion protein shows efficacy in alleviating the symptoms of CIA rats.

Key words: osteoprotegerin, heat shock protein, fusion protein, collagen-induced arthritis

中图分类号:

R318

刘姗, 余丹, 曾津津, 李慎涛, 王炜, 刘振龙, 赵文明. OPG-HSP70 融合蛋白对CIA 大鼠的保护性作用[J]. 中国临床药理学与治疗学, 2009, 14(12): 1347-1350.

LIU Shan, YU Dan, ZENG Jin-jin, LI Shen-tao, WANG Wei, LIU Zhen-long, ZHAO Wen-ming. Protective effects of OPG-HSP70 fusion protein on CIA rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(12): 1347-1350.

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