异补骨脂素防护人晶状体上皮细胞的凋亡

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (12): 1371-1374.

异补骨脂素防护人晶状体上皮细胞的凋亡

祁明信¹, 黄秀榕², 郭娜², 张可丽²

¹福建中医学院附属第二人民医院眼科,福州 350003, 福建;
²福建中医学院病理生理研究中心, 福州 350003, 福建

收稿日期:2009-08-31 修回日期:2009-12-12 发布日期:2020-10-20
通讯作者: 黄秀榕, 女, 教授, 博士生导师, 长期进行白内障的临床和基础研究。Tel:83570887 E-mail:qihuang@netease.com
作者简介:祁明信, 男, 教授, 主任医师, 博士生导师, 长期进行白内障的基础和临床研究。Tel:13178118651 E-mail:qihuang@netease.com

Isopsoralen inhibiting the apoptosis of human lens epithelial cells

QI Ming-xin¹, HUANG Xiu-rong², GUO Na², ZHANG Ke-li²

¹Department of Ophthalmology, Second Affiliated People's Hospital, Fuzhou 350003, Fujian, China;
²Research Center of Pathophysiology, Fujian College of Traditional Chinese Medicine, Fuzhou 350003, Fujian, China

Received:2009-08-31 Revised:2009-12-12 Published:2020-10-20

摘要/Abstract

摘要： 目的：探讨异补骨脂素( ISR) 对氧化损伤的人晶状体上皮细胞( HLEC-B₃ ) 凋亡有无抑制作用。方法：本研究在终浓度300 μmol L H₂O₂ 培养液中复制HLEC-B₃ 凋亡模型, 并以雌二醇( E₂ ) 作为阳性对照, 在透射电子显微镜下观察HLEC-B₃超微结构的改变, 采用FCM 法检测HLEC-B₃ 凋亡率及线粒体膜电位的变化。结果：超微结构研究显示, H₂O₂ 组绝大多数HLEC-B₃ 发生凋亡, 并呈凋亡各期改变的全过程;E₂ 、ISR 组仅少数HLECB₃发生凋亡, 并多为早期或中期的轻微改变。FCM 结果显示:H₂O₂ 组HLEC 凋亡率显著高于空白对照组;E₂ 和ISR 组凋亡率均低于H₂O₂ 组( P<0.01) 。H₂O₂ 组线粒体跨膜电位比对照组显著下降;E₂ 组和ISR 组的线粒体跨膜电位均比H₂O₂组升高( P <0.01) 。结论：ISR 可减轻实验性氧化损伤的HLEC-B₃ 凋亡程度, 为寻求防治老年性白内障的药物提供实验依据。

关键词: 抗氧化, 异补骨脂素, 白内障, 凋亡

Abstract: AIM: To investigate if isopsoralen inhibits the apoptosis of human lens epithelial cells (HLEC) with experimental oxidative injury ( HLECB₃).METHODS: Lens epithelial cells were incubated with 300 μmol L H₂0₂ to make the HLEC-B₃ model. The estradiol ( E₂ ) was as a positive control, the HLEC apoptosis and apoptosis rate were measured by FCM, the ultrastructure changes and apoptosis bodies of HLEC were observed under transmission electron microscope.RESULTS: The ultrastructure observation indicated that the apoptosis cells occurred in most HLEC of H₂0₂ group and the changes were severe and presenting different stages.While the changes in E₂ and ISR groups were slight and most of them were in early and middle stages.The apoptosis rate in H₂0₂ group was significantly higher than that in the control group, E₂ and ISR groups.The ΔΧM in H₂O₂ group was reduced obviously compared with control group, E₂ and ISR groups.CONCLUSION: ISR may decrease the apoptosis of HLEC, providing scientific evidence for pursue effective medicine to prevent and treat senile cataract.

Key words: antioxidants, Isopsoralen, Cataract, Apoptosis

中图分类号:

R965.2

祁明信, 黄秀榕, 郭娜, 张可丽. 异补骨脂素防护人晶状体上皮细胞的凋亡[J]. 中国临床药理学与治疗学, 2009, 14(12): 1371-1374.

QI Ming-xin, HUANG Xiu-rong, GUO Na, ZHANG Ke-li. Isopsoralen inhibiting the apoptosis of human lens epithelial cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(12): 1371-1374.

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