表皮生长因子对萎缩性胃炎大鼠胃黏膜上皮增生的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (2): 136-139.

表皮生长因子对萎缩性胃炎大鼠胃黏膜上皮增生的影响

袁庆丰¹, 姒健敏², 周雯², 张啸¹, 张筱凤¹, 吕文¹

1杭州市第一人民医院消化科, 杭州310006, 浙江;
²浙江大学邵逸夫临床医学研究所胃肠研究所, 杭州310016, 浙江

收稿日期:2008-09-25 修回日期:2008-12-31 出版日期:2009-02-26 发布日期:2020-10-30
作者简介:袁庆丰, 男, 医学博士, 副主任医师, 研究方向:消化道药物治疗。Tel:(0571)87065701 　E-mail:yuanqf26@yahoocom.cn。姒健敏, 男, 教授, 博士生导师, 研究方向:胃肠疾病药理机制研究。Tel:(0571)87217002 　E-mail:Sijm@163.net

Protective effect of epidermal growth factor on the atrophic gastric mucosa in rats

YUAN Qing-feng¹, SI Jian-min², ZHOU Wen², ZHANG Xiao¹, ZHANG Xiao-feng¹, LV Wen¹

¹First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang, China;
²Gastroenterologic Laboratory of Sir Run Run Shaw Clinical Institute Affiliated to Medical School of Zhejiang University, Hangzhou 310016, Zhejiang, China

Received:2008-09-25 Revised:2008-12-31 Online:2009-02-26 Published:2020-10-30

摘要/Abstract

摘要： 目的:探讨表皮生长因子(epidermal growth factor, EGF)对大鼠萎缩性胃炎(chronic atrophic gastritis, CAG)动物模型的治疗作用。方法:大鼠CAG 模型建立后, 分别给予EGF 、硫糖铝及生理盐水处理, 12 周后取出全胃, 观察各组大鼠胃黏膜病理变化、测定腺体增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)表达、测量胃黏膜腺体厚度(L1)和黏膜肌层(L2)之比及胃窦固有腺体总个数。结果:EGF 组、硫糖铝组大鼠胃黏膜腺体排列规则, 未发现有恶性增殖现象。EGF 组胃窦L1L2 值为8.6 ±1.2, 较硫糖铝组、模型组(6.6 ±1.6, 5.0 ±0.9)明显增加(P <0.05, P <0.01)。EGF 组、硫糖铝组的胃窦腺体个数值分别为(52.0±5.8)和(34.8 ±4.3), 较模型组(27.1 ±2.2)明显增加(P <0.01);EGF 组、硫糖铝组PCNA 表达阳性的高度分别为(84.5 ±2.9)μm 和(76.8 ±1.7)μm, 较模型组[ (63.3 ±9.2)μm] 明显增加(P <0.01, P <0.05), 但EGF 组的效果要优于硫糖铝组(P <0.01)。结论:EGF 对于大鼠CAG 动物模型具有恢复治疗作用, 且在促进胃腺体增殖作用方面的效果优于硫糖铝。

关键词: 表皮生长因子, 胃炎, 萎缩性, 胃黏膜, 增殖细胞核抗原

Abstract: AIM: To explore the protective effect of epidermal growth factor(EGF)on the atrophic gastric mucosa in rats.METHODS: The rats of established models of atrophic gastric mucosa were divided into 3 groups.There were treated by EGF subcutaneous injection, sucralfate by mouth and saline subcutaneous injection respectively.12 weeks later rats were executed with aether and the stomachs were removed.The gastric streak-like samples for histological examination were cut and attained with hematoxylin and eosin(HE) and with immunohistochemistry for proliferation cell nuclear antigen(PCNA)to observe a series of indexes as follows :Ratios of the thickness of gastric mucosal glands and muscularis mucosa(L1 L2);The number of gastric glands in 1 mm lengths of mucosal layer in longitudinal sections;The length of the proliferation zone represented with the length from top to bottom of PCNA positive cells.RESULTS: In the therapy groups, the gastric glands were rearranged in order and without sings of malignant proliferation.The means of L1 L2 in three groups were 8.6 ±1.2, 6.6 ±1.6 and 5.0 ±0.9 respectively.EGF significantly increased the ratios of L1 L2 than other groups(P <0.05, P <0.01).The number of gastric glands in 1 mm length of mucosal layer were 52.0 ±5.8, 34.8 ±4.3, 27.1 ±2.2 respectively.There were more number of gastric glands in EGF group and sucralfate group than that in model group (P <0.01).The means of the length from top to bottom of PCNA-positive cells were (84.5 ±2.9) μm, (76.8 ±1.7)μm and (63.3 ±9.2)μm respectively.There were more length in EGF group and sucralfate group than that in model group(P <0.05, P <0.01).CONCLUSION: EGF plays an important role in improving the gastric mucosal lesions of the rats with CAG and its effect is better than sucralfate.

Key words: epidermal growth factor, gastritis, atrophic, gastric mucosa, proliferating cell nuclear antigen

中图分类号:

R977

袁庆丰, 姒健敏, 周雯, 张啸, 张筱凤, 吕文. 表皮生长因子对萎缩性胃炎大鼠胃黏膜上皮增生的影响[J]. 中国临床药理学与治疗学, 2009, 14(2): 136-139.

YUAN Qing-feng, SI Jian-min, ZHOU Wen, ZHANG Xiao, ZHANG Xiao-feng, LV Wen. Protective effect of epidermal growth factor on the atrophic gastric mucosa in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(2): 136-139.

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