沙利度胺联合甲氨蝶呤对关节炎大鼠炎症及细胞因子的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (2): 167-170.

沙利度胺联合甲氨蝶呤对关节炎大鼠炎症及细胞因子的影响

周玮^1,2, 张育¹, 沈维干¹, 薛永骥¹, 高娜¹, 高波¹, 张学增¹

¹扬州大学临床医学院, ²扬州大学第二临床医学院　扬州市第一人民医院, 扬州225001, 江苏

收稿日期:2008-10-31 修回日期:2009-01-16 出版日期:2009-02-26 发布日期:2020-10-30
通讯作者: 张育, 男, 硕士, 教授, 主任医师, 硕士生导师, 研究方向:风湿病的基础与临床研究。Tel:0514-87978805 　E-mail:yzzy10182001@yahoo.com.cn
作者简介:周玮, 女, 硕士, 住院医师, 研究方向:风湿血液病研究。
基金资助:
江苏省科技厅资助课题(BS2005037)

Effect of thalidomide combined with methotrexate on the inflammation and cytokines of rats with collagen type II induced arthritis

ZHOU Wei^1,2, ZHANG Yu¹, SHEN Wei-gan¹, XUE Yong-ji¹, GAO Na¹, GAO Bo¹, ZHANG Xue-zhen¹

¹Clinic Medical College of Yangzhou University, ²the Second Clinic Medical College of Yangzhou University, Yangzhou No.1 People's Hospital, Yangzhou 225001, Jiangsu, China

Received:2008-10-31 Revised:2009-01-16 Online:2009-02-26 Published:2020-10-30

摘要/Abstract

摘要： 目的:研究沙利度胺联合甲氨蝶呤对大鼠II 型胶原诱导型关节炎症及细胞因子的影响。方法:建立类风湿性关节炎大鼠模型, 自造模次日治疗组分别给予沙利度胺(150 mg·kg^-1·d^-1) 联合甲氨蝶呤(0.2 mg·kg^-1 ·d^-1) 和甲氨蝶呤(0.2 mg·kg^-1·d^-1) 单用, 疗程均为6 周。各组大鼠每周进行一次关节炎指数(AI) 评分, 于造模第6 周取血清进行Western Blot 检测大鼠体内IL-1β 、TNF-α的表达并计算相对含量。结果:沙利度胺联合甲氨蝶呤、单用甲氨蝶呤均能降低模型大鼠AI 评分, 沙利度胺联合甲氨蝶呤作用强于单独使用甲氨蝶呤, 使AI 评分明显下降。沙利度胺联合甲氨蝶呤、甲氨蝶呤均可抑制IL-1β 、TNF-α的表达, 治疗后两种因子表达均与模型组有统计学差异(P <0.05), 但仍不能达到正常水平(P <0.05)。结论:沙利度胺联合甲氨蝶呤可能通过抑制IL-1β 、TNF-α的表达而达到抗关节炎症的作用,沙利度胺与甲氨蝶呤有协同作用。

关键词: 沙利度胺, 甲氨蝶呤, 大鼠, 关节炎, II 型胶原

Abstract: AIM: To investigate the effect of thalidomide combined with methotrexate on the inflammation and cytokines of rats with collagen type II induced arthritis.METHODS: The model of rat was induced by injection of collagen type II combined with complete Freund's adjuvant (CFA).Next day after the injection, the therapeutic administration of thalidomide(150 mg·kg^-1 ·d^-1) combined with methotrexate (0.2 mg·kg^-1 ·d^-1) and methotrexate(0.2 mg·kg^-1·d^-1) were differently performed to the rats.The courses of the treatment were all six weeks.The mean arthritis index (AI) was scored every week.The relative concentrations of IL-1β, TNF-αin sera were evaluated by Western Blotting.RESULTS: The therapeutic administration of thalidomide combined with methotrexate and methotrexate could decrease the mean arthritis index on CIA rat, the effect of thalidomide combined with methotrexate was more obvious than that of methotrexate. The administration of thalidomide combined with methotrexate and methotrexate significantly decreased the production of IL-1β and TNF-α, the change of posttreatmentis was obviously in all groups (P <0.05). CONCLUSION: Thalidomide combined with methotrexate have an inhibitory effect on the expression of IL- 1β and TNF-αin model rat, which is related to its anti- inflammation.There are coordinate effect in thalidomide and methotrexate.

Key words: thalidomide, methotrexate, rat, arthritis, collagen type II

中图分类号:

R965.2

周玮, 张育, 沈维干, 薛永骥, 高娜, 高波, 张学增. 沙利度胺联合甲氨蝶呤对关节炎大鼠炎症及细胞因子的影响[J]. 中国临床药理学与治疗学, 2009, 14(2): 167-170.

ZHOU Wei, ZHANG Yu, SHEN Wei-gan, XUE Yong-ji, GAO Na, GAO Bo, ZHANG Xue-zhen. Effect of thalidomide combined with methotrexate on the inflammation and cytokines of rats with collagen type II induced arthritis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(2): 167-170.

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